Recombinant Human Anoctamin-10 (ANO10), partial

Code CSB-YP873667HU
MSDS
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Source Yeast
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Code CSB-EP873667HU
MSDS
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Source E.coli
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Code CSB-EP873667HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP873667HU
MSDS
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Source Baculovirus
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Code CSB-MP873667HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ANO10
Uniprot No.
Alternative Names
ANO10; TMEM16KAnoctamin-10; Transmembrane protein 16K
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Does not exhibit calcium-activated chloride channel (CaCC) activity. Can inhibit the activity of ANO1.
Gene References into Functions
  1. This study describe 2 Romani families from Serbia presenting with seemingly dominant (multiple affected individuals in successive generations) cerebellar ataxia, both harboring the same homozygous (recessive) mutation in ANO10, identified by whole-exome sequencing. PMID: 27787937
  2. mutations in ANO10 cause cellular defects and genetic disorders through deranged local Ca(2+) signaling. PMID: 27838374
  3. results suggest that executive and attentional disorders are impaired in ANO10 mutation PMID: 27045840
  4. ANO10 has a central role in innate immune defense against Borrelia infection. PMID: 25730773
  5. The detection of mutations in ANO10 indicate that ANO10 defects cause secondary low coenzyme Q10. PMID: 25182700
  6. An ANO10 mutation is responsible for autosomal recessive cerebellar ataxia that is mainly characterized by cerebellar atrophy and lack of peripheral neuropathy. PMID: 25089919
  7. Whole-exome and targeted sequencing have defined the genetic basis of dizziness including new genes causing ataxia: GBA2, TGM6, ANO10 and SYT14 PMID: 24275721
  8. This study report Gypsy family with autosomal recessive ataxia caused by the same truncating ANO10 defect. PMID: 22008874
  9. New DNA sequencing technologies are enabling us to investigate the whole or large targeted proportions of the genome in a rapid, affordable, and comprehensive way. Exome and targeted sequencing ANO10 genes causing ataxia. PMID: 22527681

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Involvement in disease
Spinocerebellar ataxia, autosomal recessive, 10 (SCAR10)
Subcellular Location
Cell membrane; Multi-pass membrane protein. Note=Shows predominantly an intracellular localization with a weak expression in the cell membrane.
Protein Families
Anoctamin family
Tissue Specificity
Highly expressed in the brain. Intermediate levels in the retina and heart and low levels in the placenta, liver, lung, duodenum, kidney, testis and spleen. In brain areas, highest expression in the frontal and occipital cortices and in the cerebellum. Lo
Database Links

HGNC: 25519

OMIM: 613726

KEGG: hsa:55129

STRING: 9606.ENSP00000292246

UniGene: Hs.656657

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