Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Target Names

SPAG9

Uniprot No.

O60271

Research Area

Developmental Biology

Alternative Names

(JIP-4)(JNK-interacting protein 4)(Cancer/testis antigen 89)(CT89)(Human lung cancer oncogene 6 protein)(HLC-6)(JNK-associated leucine-zipper protein)(JLP)(Mitogen-activated protein kinase 8-interacting protein 4)(Proliferation-inducing protein 6)(Protein highly expressed in testis)(PHET)(Sperm surface protein)(Sperm-associated antigen 9)(Sperm-specific protein)(Sunday driver 1)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

408-534aa

Target Protein Sequence

REVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEM
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

20.0 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 408-534 constitute the expression domain of recombinant Human SPAG9. The expected molecular weight for the SPAG9 protein is calculated to be 20.0 kDa. The SPAG9 protein was expressed in e.coli. The SPAG9 coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant SPAG9 protein in following stages of expression and purification.

C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9) is known to interact with c-Jun N-terminal kinase (JNK), a key component of the mitogen-activated protein kinase (MAPK) signaling pathway. This interaction suggests a role for SPAG9 in mediating cellular responses to extracellular signals, including those related to cell growth, differentiation, and apoptosis. Additionally, SPAG9 has been implicated in the regulation of microtubule dynamics, contributing to cell migration and invasion. Its overexpression has been observed in certain cancers, suggesting a potential role in tumorigenesis.

Target Background

Function

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex. Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking.

Gene References into Functions

AKAP4 and SPAG9 genes may find use as diagnostic biomarkers for CRC. PMID: 29480665
Aberrant expression of JNK-associated leucine-zipper protein, JLP, promotes accelerated growth of ovarian cancer PMID: 27655714
SPAG9 mRNA and protein overexpression in lung cancer tissue, and the presence of SPAG9 IgG antibody in peripheral blood of lung cancer patients indicates that it has potential as a biomarker for lung cancer diagnosis. PMID: 26934841
SPAG9-elevated expression contributes to malignant behavior and poor prognosis of breast cancer and may support a potential indicator in treatment selection. PMID: 26631164
Study suggest that suppression of miR-141 may cause an aberrant overexpression of SPAG9 promoting growth and metastasis of hepatocellular carcinoma cells. PMID: 26790956
expression of SPAG9 in ECC cells with TGF-beta1 treatment and spheroids formation was increased PMID: 27352556
PLK1 binding to JIP4 was found in G2 phase and mitosis, and PLK1 binding was self-primed by PLK1 phosphorylation of JIP4. PMID: 26291670
Based on in vitro assays, we found miR-200a-3p significantly inhibit cancer cell proliferation by inducing apoptosis. PMID: 26797273
FOXK1 protein levels and activity are regulated by associating with JLP and PLK1 PMID: 26468278
SPAG9 overexpression in gastric cancer correlates with poor prognosis and contributes to gastric cancer cell proliferation PMID: 26293216
SPAG9 was upregulated in nonmelanoma skin cancer when compared with normal skin. PMID: 25033008
SPAG9 expression is significantly increased in prostate cancer and it may be involved in the process of prostate cancer cell motility, migration and angiogenesis. PMID: 25310386
knockdown of Sec8 enhances the binding of JIP4 to MAPK kinase 4, thereby decreasing the phosphorylation of MAPK kinase 4, JNK, and p38. PMID: 25244576
SPAG9 upregulates PODXL expression in human astrocytoma cells at the PODXL gene promoter/transcriptional level through a JNKdependent mechanism. PMID: 24788963
SPAG9 is overexpressed in human HCC and serves as a prognostic marker. SPAG9 contributes to cancer cell growth through regulation of cyclin proteins. PMID: 24801907
SPAG9 is overexpressed in human prostate cancers and contributes to prostate cancer cell growth, possibly through cyclin protein regulation. PMID: 24740566
down regulation of SPAG9 reduces growth and invasive potential of triple-negative breast cancer cells, suggesting that SPAG9 may be a potential target for therapeutic use. PMID: 24330581
data in clinical specimens indicated that SPAG9 is potential biomarker and therapeutic target for bladder TCC. PMID: 24349057
High SPAG9 expression is associated with endometrial cancer. PMID: 24460345
SPAG9 depletion could inhibit the activity of p-JNK. PMID: 23711689
SPAG9 serves as an important oncoprotein in human astrocytoma by regulating cell proliferation and invasion. PMID: 23696027
SPAG9 is positively expressed in endometrial cancer, and with a high humoral immune response in patients. It may serve as a new type of endometrial cancer markers for early detection, diagnosis and treatment. PMID: 22146769
These findings collectively suggest that SPAG9 may have a role in tumor development and early spread. PMID: 21356354
N-cadherin expression had an inhibitory effect on JLP-mediated p38 MAPK signal activation by decreasing the interaction between JLP and p38 MAPK PMID: 21177868
SPAG9 mRNA& protein are expressed in CML patients (88%) and in K562 & KCL-22 cells. 90% CML chronic-phase patients showed humoral response against SPAG9. PMID: 20138665
SPAG9 protein is found in the equatorial plate and flagella of human spermatozoa. PMID: 19947555
Testicular antigen PHET is an auutoantigen recognized by sera from systemic sclerosis (SSc) patients with extensive fibrotic changes; the autoantibody response to PHET is induced by ectopic overexpression of PHET in dermal fibroblasts of SSc patients. PMID: 14662895
first report of sperm-associated JNK-binding protein (SPAG9) that may have a role in spermatozoa-egg interaction PMID: 15693750
SPAG9 is a new member of c-Jun NH2 -terminal kinase (JNK) interacting protein exclusively expressed in testis PMID: 16077255
The present investigation will eventually extend the application of SPAG9 siRNA in in vivo targeting experiments that aim to define the SPAG9 functional genomics in tumor and reproductive biology. PMID: 16356479
Recombinant human protein is adsorbed on alum is highly immunogenic in macaca model and therefore represents a suitable sperm-based vaccine immunogen for fertility trials in macaque. PMID: 16959808
SPAG9 may have a role in tumor development and metastasis and thus could serve as a novel target for early detection and treatment of renal cell carcinoma. PMID: 18922895
Kinesin adapter JLP links PIKfyve to microtubule-based endosome-to-trans-Golgi network traffic of furin. PMID: 19056739
structural analysis of ARF6 in a complex with JIP4 PMID: 19644450
Findings support a potential role for SPAG9 as diagnostic biomarker as well as a possible therapeutic target in thyroid cancer treatment. PMID: 19820019

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Subcellular Location

Cytoplasm. Cytoplasm, perinuclear region. Lysosome membrane.; [Isoform 5]: Cytoplasmic vesicle, secretory vesicle, acrosome.

Protein Families

JIP scaffold family

Tissue Specificity

Expressed only in testis on the round spermatids of stage I, II and II. Absent in spermatogonia and spermatocyte.; [Isoform 4]: Expressed in testis and in acute myeloid leukemia (AML) patients.; [Isoform 3]: Expressed in testis.

Database Links

HGNC: 14524

OMIM: 605430

KEGG: hsa:9043

STRING: 9606.ENSP00000262013

UniGene: Hs.463439

Code	CSB-EP022470HU
Abbreviation	Recombinant Human SPAG9 protein, partial
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9), partial

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