Recombinant Human Chromodomain-helicase-DNA-binding protein 8(CHD8) ,partial

Code CSB-YP872548HU
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Source Yeast
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Code CSB-EP872548HU
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Source E.coli
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Code CSB-EP872548HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872548HU
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Source Baculovirus
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Code CSB-MP872548HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names CHD8
Uniprot No. Q9HCK8
Alternative Names ATP dependent helicase CHD8; ATP-dependent helicase CHD8; Axis duplication inhibitor; Beta catenin binding protein like; CHD 8; CHD-8; chd8; CHD8_HUMAN; Chromodomain helicase DNA binding protein 8; Chromodomain-helicase-DNA-binding protein 8; DKFZp686N17164; Duplin; Helicase with SNF2 domain 1; HELSNF1 ; KIAA1564
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription.
Gene References into Functions
  1. The Autism spectrum disorder candidate genes SATB2, CHD8 and EHMT1 show enriched expression in neurons, especially inhibitory neurons PMID: 29317598
  2. SHANK3, CHD8, and ADNP had distinctly higher scores than all other genes in the dataset describing the genes associated with autism spectrum disorders. PMID: 27790361
  3. present observation and published data suggest that phenotype present in patients with duplication of 14q11.2 region, encompassing the SUPT16H and CHD8 genes, resemble in some extend features described in cases carrying microdeletion of that genomic region PMID: 26834018
  4. Review of 16 other CHD8 mutation cases suggests that clinical features and their severity vary considerably across individuals; however, these data support a CHD8 mutation syndrome, further highlighting the importance of genomic medicine to guide clinical assessment and treatment PMID: 26789910
  5. Our clinical case supports the hypothesis that CHD8 may play a central role in neuronal cell development and Autism spectrum disorders risk PMID: 26921529
  6. Study provides evidence that links the CHD8 chromatin remodeler to the cancer maintenance functions of BRD4 and NSD3. PMID: 26626481
  7. Taken together our data demonstrate that CHD8 is involved in late stages of progesterone receptor enhancers activation. PMID: 25894978
  8. Loss of CHD8 contributes to autism spectrum disorder by perturbing an ancient gene regulatory network during human brain development. PMID: 25752243
  9. CHD8 insufficiency results in altered gene expression of coding genes and noncoding RNAs. The changes among protein-coding genes involved genes that are enriched in neuronal development and in previously identified autism candidate genes. PMID: 25989142
  10. In the first detailed analysis in cancer, a marked loss of CHD8 expression and increased BORIS/CTCF ratio indicate frequent disruption of CTCF and its effector genes in PCa. PMID: 25499215
  11. Recurrent approximately 100 Kb microdeletion in the chromosomal region 14q11.2, involving CHD8 gene, is associated with autism and macrocephaly. PMID: 25257502
  12. CHD8 precipitates a network of gene-expression changes involved in neurodevelopmental pathways in which many autism spectrum disorder-associated genes may converge on shared mechanisms of pathogenesis PMID: 25294932
  13. indings suggest that CHD8 disruptions represent a specific pathway in the development of Autism spectrum disorder (ASD) and define a distinct ASD subtype. Common phenotypic features include increased head size; a facial phenotype marked by prominent forehead, wide-set eyes, and pointed chin; as well as increased rates of GI complaints and marked sleep dysfunction. PMID: 24998929
  14. CHD8 is required for E2F-dependent transcription activation of S-phase genes PMID: 24265227
  15. Loss of CHD8 expression is associated with aggressiveness in gastric cancer. PMID: 23835524
  16. results from de novo events and a large parallel case-control study provide strong evidence in favour of CHD8 and KATNAL2 as genuine autism risk factors PMID: 22495311
  17. CHD8 promotes the association of beta-catenin and histone H1, with formation of the trimeric complex on chromatin being required for inhibition of beta-catenin-dependent transactivation. PMID: 22083958
  18. The recruitment of AR to the TMPRSS2 promoter in response to androgen treatment requires CHD8. PMID: 20308527
  19. This study provides evidence of a novel interaction between BRCA1 and beta-catenin, and that loss of BRCA1 leads to impaired expression of the nuclear form of beta-catenin, which may contribute to the pathogenesis of breast cancer. PMID: 20215423
  20. Results show that Depletion of CHD8 enhances HOXA2 expression and a loss of the WDR5/Ash2L/RbBP5 subcomplex. PMID: 20085832
  21. Staf activates U6 transcription from a preassembled chromatin template in vitro and associates with several proteins linked to chromatin modification, among them chromodomain-helicase-DNA binding protein 8 (CHD8). PMID: 17938208
  22. CHD8 is an ATP-dependent chromatin remodeling factor that regulates beta-catenin target genes PMID: 18378692
  23. CHD8 interacts with elongating RNA polymerase II and controls expression of CCNE2 and TYMS genes. PMID: 19255092

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Involvement in disease Autism 18 (AUTS18)
Subcellular Location Nucleus
Protein Families SNF2/RAD54 helicase family, CHD8 subfamily
Database Links

HGNC: 20153

OMIM: 610528

KEGG: hsa:57680

STRING: 9606.ENSP00000382863

UniGene: Hs.530698

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