Recombinant Human DNA-directed primase/polymerase protein (PRIMPOL)

1. RPA serves to stimulate the primase activity of PrimPol. PMID: 28534480
2. These new findings supports the existence of a functional PrimPol/RPA association that allows repriming at the exposed ssDNA regions formed in the leading strand upon replicase stalling. PMID: 28396594
3. PrimPol shows the ability to synthesize DNA opposite ultraviolet (UV) lesions; however, unexpectedly, the active-site cleft of the enzyme is constrained, which precludes the bypass of UV-induced DNA lesions by conventional translesion synthesis. PMID: 27819052
4. Growing evidence suggests that the main biological function of human PrimPol during replication of chromosomal DNA is a repriming of stalled replication downstream of DNA damage or naturally occurring obstacles. However, the mechanisms that regulate the repriming by PrimPol in cells are yet to be understood. PrimPol in cells are yet to be understood. [review] PMID: 28754021
5. Active PrimPol can be purified from E. coli and human suspension cell line in high quantities and the activity of the purified enzyme is similar in both expression systems. PMID: 28902865
6. The ability of human PrimPol to discriminate against ribonucleotides (rNTPs) and to incorporate the triphosphates of four nucleoside analog drugs in the presence of Mn2+or Mg2+ was studied. PMID: 27989484
7. Data propose that it is highly likely that PrimPol plays the same roles in mitochondria as in cell nucleus by repriming DNA replication to allow replication to be completed in an efficient and timely manner. Also, a range of PrimPol mutations have been found in cancer cells and other conditions suggesting possible connections to human diseases. [review] PMID: 28408491
8. The molecular mechanism of polymerization and nucleoside reverse transcriptase inhibitor incorporation by human PrimPol have been described. PMID: 26552983
9. findings establish that PolDIP2 can regulate the translesion synthesis polymerase and primer extension activities of PrimPol PMID: 26984527
10. Data suggest that, during genetic transcription, Prim-Pol-alpha-cat binds the DNA/RNA junction at 5prime-terminus of RNA primer (or initiating NTP, nucleoside-triphosphate). PMID: 26710848
11. Implicate PrimPol in promoting restart of DNA synthesis downstream of, but closely coupled to, G4 replication impediments. PMID: 26626482
12. Rad51 recombinase prevents Mre11 nuclease-dependent degradation and excessive PrimPol-mediated elongation of nascent DNA after UV irradiation PMID: 26627254
13. PrimPol tolerates DNA lesions, involving template and primer dislocations that can be operating during both mitochondrial and nuclear DNA replication. PrimPol could also operate as a translesion synthesis partner during DNA-directed RNA synthesis. PMID: 25746449
14. PRIMPOL depletion results in increased spontaneous DNA damage and defects in the restart of stalled replication forks. PMID: 24126761
15. The authors propose a mechanism whereby single-stranded DNA binding proteins greatly restrict the contribution of PrimPol to DNA replication at stalled forks, thus reducing the mutagenic potential of PrimPol during genome replication. PMID: 25550423
16. The data establishes that a point mutation identified in PrimPol from patients with high myopia results in a major disruption of the catalytic and replication activities associated with human PrimPol thus establishing a link between replication stress and high myopia. PMID: 25262353
17. Data suggest that PrimPol exhibits fidelity that is 1.7-fold more accurate with magnesium as cofactor compared to manganese; activity of PrimPol is increased 400-1000-fold by manganese compared to magnesium based on steady-state kinetic parameters. PMID: 25255211
18. Although PrimPol's primase activity is required to restore wild-type replication fork rates in irradiated PrimPol-/- cells, the polymerase activity is sufficient to maintain regular replisome progression in unperturbed cells. PMID: 24682820
19. Identification of a novel missense variant of the CCDC111 gene in a high myopia family. PMID: 23579484
20. PrimPol is an important player in replication fork progression in eukaryotic cells. PMID: 24267451
21. Primpol is proposed to facilitate replication fork progression by acting as a translesion DNA polymerase or as a specific DNA primase reinitiating downstream of lesions that block synthesis during DNA replication. PMID: 24207056

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HGNC: 26575

OMIM: 615420

KEGG: hsa:201973

STRING: 9606.ENSP00000313816

UniGene: Hs.481307