Recombinant Human Coiled-coil domain-containing protein 114 (CCDC114)

Code CSB-YP856980HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP856980HU
MSDS
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Source E.coli
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Code CSB-EP856980HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856980HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP856980HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CCDC114
Uniprot No.
Alternative Names
CCDC114; Coiled-coil domain-containing protein 114
Species
Homo sapiens (Human)
Expression Region
1-670
Target Protein Sequence
MEGERRAYSK EVHQRINKQL EEIRRLEEVR GDLQVQISAA QNQVKRLRDS QRLENMDRLL KGRAQVQAEI EELQEQTRAL DKQIQEWETR IFTHSKNVRS PGFILDQKVK IRRRIRILEN QLDRVTCHFD NQLVRNAALR EELDLLRIDR NRYLNVDRKL KKEIHHLHHL VSTLILSSTS AYAVREEAKA KMGLLRERAE KEEAQSEMEA QVLQRQILHL EQLHHFLKLK NNDRQPDPDV LEKREKQAGE VAEGVWKTSQ ERLVLCYEDA LNKLSQLMGE SDPDLLVQKY LEIEERNFAE FNFINEQNLE LEHVQEEIKE MQEALVSARA SKDDQHLLQE QQQKVLQQRM DKVHSEAERL EARFQDVRGQ LEKLKADIQL LFTKAHCDSS MIDDLLGVKT SMGDRDMGLF LSLIEKRLVE LLTVQAFLHA QSFTSLADAA LLVLGQSLED LPKKMAPLQP PDTLEDPPGF EASDDYPMSR EELLSQVEKL VELQEQAEAQ RQKDLAAAAA KLDGTLSVDL ASTQRAGSST VLVPTRHPHA IPGSILSHKT SRDRGSLGHV TFGGLSSSTG HLPSHITHGD PNTGHVTFGS TSASSGGHVT FRPVSASSYL GSTGYVGSSR GGENTEGGVE SGGTASDSSG GLGSSRDHVS STGPASSTGP GSSTSKDSRG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the outer dynein arm-docking complex (ODA-DC) that mediates outer dynein arms (ODA) binding onto the doublet microtubule.
Gene References into Functions
  1. These results revealed that mutations in CCDC114 are a cause of ciliary dysmotility and PCD and further demonstrate the utility of exome sequencing to identify genetic causes in heterogeneous recessive disorders. PMID: 23261302
  2. Splice-site mutations in the axonemal outer dynein arm docking complex gene CCDC114 cause primary ciliary dyskinesia PMID: 23261303
Involvement in disease
Ciliary dyskinesia, primary, 20 (CILD20)
Subcellular Location
Cell projection, cilium. Cytoplasm, cytoskeleton, cilium axoneme.
Protein Families
ODA1/DCC2 family
Database Links

HGNC: 26560

OMIM: 615038

KEGG: hsa:93233

STRING: 9606.ENSP00000318429

UniGene: Hs.112645

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