Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

CRY2

Uniprot No.

Q49AN0

Research Area

Cardiovascular

Alternative Names

cry2; CRY2_HUMAN; cryptochrome 2 (photolyase like); Cryptochrome 2; Cryptochrome-2; FLJ10332; growth inhibiting protein 37; HCRY2; KIAA0658; PHLL2; Photolyase like

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-593aa

Target Protein Sequence

MAATVATAAAVAPAPAPGTDSASSVHWFRKGLRLHDNPALLAAVRGARCVRCVYILDPWFAASSSVGINRWRFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAGVEVVTENSHTLYDLDRIIELNGQKPPLTYKRFQAIISRMELPKKPVGLVTSQQMESCRAEIQENHDETYGVPSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFGCLSCRLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWAEGKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSVNAGSWMWLSCSAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKAFPSRYIYEPWNAPESIQKAAKCIIGVDYPRPIVNHAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPSSSQAGSMSSAGPRPLPSGPASPKRKLEAAEEPPGEELSKRARVAELPTPELPSKDA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

74.4 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-593 constitute the expression domain of recombinant Human CRY2. The calculated molecular weight for this CRY2 protein is 74.4 kDa. This CRY2 protein is produced using e.coli expression system. Fusion of the N-terminal 10xHis tag and C-terminal Myc tag into the CRY2 encoding gene fragment was conducted, allowing for easier detection and purification of the CRY2 protein in subsequent stages.

Human cryptochrome-2 (CRY2) is a core component of the circadian clock, regulating the sleep-wake cycle and biological rhythms. CRY2, alongside CRY1, interacts with the CLOCK-BMAL1 complex, influencing transcriptional repression during the circadian cycle. In chronobiology, CRY2 is pivotal for maintaining circadian rhythms and synchronizing internal processes with environmental cues. Research on CRY2 extends to metabolic disorders, as it affects energy metabolism and glucose homeostasis. Additionally, CRY2 is involved in DNA repair and cancer biology, influencing tumor suppression mechanisms. Studying CRY2 provides insights into circadian regulation, metabolism, and cancer pathways, offering potential applications in treating circadian-related disorders, metabolic diseases, and cancer therapeutics.

Target Background

Function

Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. CRY1 and CRY2 have redundant functions but also differential and selective contributions at least in defining the pace of the SCN circadian clock and its circadian transcriptional outputs. Less potent transcriptional repressor in cerebellum and liver than CRY1, though less effective in lengthening the period of the SCN oscillator. Seems to play a critical role in tuning SCN circadian period by opposing the action of CRY1. With CRY1, dispensable for circadian rhythm generation but necessary for the development of intercellular networks for rhythm synchrony. May mediate circadian regulation of cAMP signaling and gluconeogenesis by blocking glucagon-mediated increases in intracellular cAMP concentrations and in CREB1 phosphorylation. Besides its role in the maintenance of the circadian clock, is also involved in the regulation of other processes. Plays a key role in glucose and lipid metabolism modulation, in part, through the transcriptional regulation of genes involved in these pathways, such as LEP or ACSL4. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by binding to glucocorticoid response elements (GREs). Represses the CLOCK-ARNTL/BMAL1 induced transcription of BHLHE40/DEC1. Represses the CLOCK-ARNTL/BMAL1 induced transcription of NAMPT. Represses PPARD and its target genes in the skeletal muscle and limits exercise capacity. Represses the transcriptional activity of NR1I2.

Gene References into Functions

CRY2 levels (ng/mL) were markedly higher in both Metabolic syndrome (MetS) groups (non-diabetic and pre-diabetic/diabetic) (all with p-value < 0.001). A reciprocal melatonin-CRY2 relationship was observed in the MetS (non-diabetic) group (p-value = 0.003). PMID: 29813030
CRY2 may be an anti-oncogene in osteosarcoma. PMID: 29879092
data indicate an oncogenic role of miR-181d in CRC by promoting glycolysis, and miR-181d/CRY2/FBXL3/c-myc feedback loop might be a therapeutic target for patients with CRC. PMID: 28749470
In the longitudinal analysis, CRY2 SNP rs61884508 was protective from worsening of problematicity of seasonal variations of mood disorder. In the cross-sectional analysis, CRY2 SNP rs72902437 showed evidence of association with problematicity of seasonal variations, as did SNP rs1554338 (in the MAPK8IP1 and downstream of CRY2). PMID: 27267441
The earlier reported association of CRY2 variants with dysthymia was confirmed and extended to major depressive disorder. PMID: 27721187
These results demonstrate that CRY2 stability controlled by FBXL3 plays a key role in the regulation of human sleep wake behavior. PMID: 27529127
The FOXM1 is a negative regulator of CRY2 in breast cancer via enhancing methylation in CRY2 promoter and its high expression is an independent predictor of favorable MR-free survival in ER+ breast cancer patients. PMID: 28579430
CRY2 and FBXL3 cooperatively degrade c-MYC preventing the development of cancer. PMID: 27840026
For the first time, we show that Cry 2 rs2292910 and MTNR1B rs3781638 are associated with osteoporosis in a Chinese geriatric cohort. PMID: 26564225
data may point to CRY2 as a novel switch in hepatic fuel metabolism promoting triglyceride storage and, concomitantly, limiting glucose production PMID: 26726810
Data indicate that cryptochrome 2 (CRY2) knockdown leads to chemosensitivity of colorectal cancer cell lines. PMID: 25855785
CRY2 and REV-ERB ALPHA as the clock genes upregulated in obesity during the 24 h period and that REV-ERB ALPHA is an important gene associated with MS. PMID: 25365257
These findings suggest that the core circadian gene CRY2 is associated with breast cancer progression and prognosis, and that knockdown of CRY2 causes the epigenetic dysregulation of genes involved in cancer-relevant pathways PMID: 25740058
CRY2 genetic variants associate with the depressive episodes in a range of mood disorder; CRY2 is hypothesized to a key to the resetting of clocks throughout and play a leading role in the antidepressant effect of total sleep deprivation PMID: 24099037
Four CRY2 genetic variants (rs10838524, rs7121611, rs7945565, rs1401419) associated significantly with dysthymia (false discovery rate q<0.05). PMID: 23951166
Our data indicate that variants in the circadian-related genes CRY2 and MTNR1B may affect long-term changes in energy expenditure, and dietary fat intake may modify the genetic effects. PMID: 24335056
These data document for the first time that the expression of BMAL1, PER3, PPARD and CRY2 genes is altered in gestational diabetes compared to normal pregnant women. deranged expression of clock genes may play a pathogenic role in GDM. PMID: 23323702
Reduced cry2 expression is associated with glioma. PMID: 23317246
Genetic variants of CRY2 associate with seasonal affective disorder or winter depression. PMID: 22538398
the associations of GLIS3-rs7034200 and CRY2-rs11605924 with fasting glucose, beta cell function, and type 2 diabetes PMID: 21747906
Human CRY2 has the molecular capability to function as a light-sensitive magnetosensor. PMID: 21694704
Lean individuals exhibited significant (P < 0.05) temporal changes of core clock (PER1, PER2, PER3, CRY2, BMAL1, and DBP) and metabolic (REVERBalpha, RIP140, and PGC1alpha) genes. PMID: 21411511
CRY2 gene variation was associated with bipolar disorder. PMID: 20856823
Data suggest that CRY2 locus is associated with vulnerability for depression. PMID: 20195522
findings suggest a role for CRY2 in breast tumorigenesis PMID: 20233903
Data suggest a limited, but potentially important role for CRY2 in the regulation of DNA damage repair and the maintenance of genomic stability in breast cancer. PMID: 20334671
CRY2 binds to DNA and in particular to single-stranded DNA with moderately high affinity and to DNA containing (6-4) photoproducts with higher affinity, but it has no DNA repair activity. PMID: 12627958
Transcripts of hCry2 underwent circadian oscillation. The expression profile of hCry2 was similar to that of hPer3. PMID: 14750904
The carboxyl-terminal domain of human cryptochrome 2 is intrinsically unstructured in solution, consistent with its presumed role as a signal transduction domain. PMID: 15751956
A clear circadian rhythm was shown for hPer1, hPer2, and hCry2 expression in CD34(+) cells and for hPer1 in the whole bone marrow, with maxima from early morning to midday. PMID: 17440215
The expression level of CRY2 was decreased in hepatrocellular carcinoma. PMID: 18444243
Both genetic association and functional analyses suggest that the circadian gene CRY2 may play an important role in NHL development. PMID: 19318546

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Subcellular Location

Cytoplasm. Nucleus. Note=Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.

Protein Families

DNA photolyase class-1 family

Tissue Specificity

Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skele

Database Links

HGNC: 2385

OMIM: 603732

KEGG: hsa:1408

STRING: 9606.ENSP00000406751

UniGene: Hs.532491

Code	CSB-EP673229HU
Abbreviation	Recombinant Human CRY2 protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Cryptochrome-2 (CRY2)

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