Recombinant Human DDB1- and CUL4-associated factor 11 (DCAF11)

Code CSB-YP851558HU
MSDS
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Source Yeast
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Code CSB-EP851558HU
MSDS
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Source E.coli
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Code CSB-EP851558HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP851558HU
MSDS
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Source Baculovirus
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Code CSB-MP851558HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DCAF11
Uniprot No.
Alternative Names
DCAF11; WDR23; GL014; PRO2389DDB1- and CUL4-associated factor 11; WD repeat-containing protein 23
Species
Homo sapiens (Human)
Expression Region
1-546
Target Protein Sequence
MGSRNSSSAG SGSGDPSEGL PRRGAGLRRS EEEEEEDEDV DLAQVLAYLL RRGQVRLVQG GGAANLQFIQ ALLDSEEEND RAWDGRLGDR YNPPVDATPD TRELEFNEIK TQVELATGQL GLRRAAQKHS FPRMLHQRER GLCHRGSFSL GEQSRVISHF LPNDLGFTDS YSQKAFCGIY SKDGQIFMSA CQDQTIRLYD CRYGRFRKFK SIKARDVGWS VLDVAFTPDG NHFLYSSWSD YIHICNIYGE GDTHTALDLR PDERRFAVFS IAVSSDGREV LGGANDGCLY VFDREQNRRT LQIESHEDDV NAVAFADISS QILFSGGDDA ICKVWDRRTM REDDPKPVGA LAGHQDGITF IDSKGDARYL ISNSKDQTIK LWDIRRFSSR EGMEASRQAA TQQNWDYRWQ QVPKKAWRKL KLPGDSSLMT YRGHGVLHTL IRCRFSPIHS TGQQFIYSGC STGKVVVYDL LSGHIVKKLT NHKACVRDVS WHPFEEKIVS SSWDGNLRLW QYRQAEYFQD DMPESEECAS APAPVPQSST PFSSPQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Gene References into Functions
  1. Here, the authors demonstrated that CUL4B forms an E3 ligase with RBX1 (RING-box 1), DDB1 (DNA damage binding protein 1), and DCAF11 (DDB1 and CUL4 associated factor 11) that promotes the ubiquitination of p21(Cip1) and regulates cell cycle progression in human osteosarcoma cells. PMID: 28446751
  2. CRL4(WDR23) is required for efficient histone mRNA 3' end processing to produce mature histone mRNAs for translation. CRL4(WDR23) binds and ubiquitylates SLBP in vitro and in vivo, and this modification activates SLBP function in histone mRNA 3' end processing without affecting its protein levels. PMID: 27203182
  3. CRL4-DCAF11 mediates the degradation of SLBP at the end of S phase and this degradation is essential for the viability of cells. PMID: 27254819
  4. ur results identify WDR23 as an alternative regulator of NRF2 proteostasis and uncover a cellular pathway that regulates NRF2 activity and capacity for cytoprotection independently of KEAP1. PMID: 28453520
  5. Includes the functional characterization of a similar protein in C. elegans and comparison to the human protein. PMID: 19273594

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Database Links

HGNC: 20258

OMIM: 613317

KEGG: hsa:80344

STRING: 9606.ENSP00000415556

UniGene: Hs.525251

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