Recombinant Human DNA excision repair protein ERCC-6-like (ERCC6L), partial

Code CSB-YP650698HU
MSDS
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Source Yeast
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Code CSB-EP650698HU
MSDS
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Source E.coli
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Code CSB-EP650698HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP650698HU
MSDS
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Source Baculovirus
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Code CSB-MP650698HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ERCC6L
Uniprot No.
Alternative Names
ATP dependent helicase ERCC6 like; ATP-dependent helicase ERCC6-like; DNA excision repair protein ERCC 6 like; DNA excision repair protein ERCC-6-like; ERC6L_HUMAN; ERCC 6L; Ercc6l; Excision repair cross complementing rodent repair deficiency complementation group 6 like; Excision repair protein ERCC6 like; FLJ20105; MGC131695; PICH; Plk1 interacting checkpoint helicase; PLK1-interacting checkpoint helicase; SNF2/RAD54 family protein; Tumor antigen BJ HCC 15; Tumor antigen BJ-HCC-15
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DNA helicase that acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. Functions as ATP-dependent DNA translocase. Can promote Holliday junction branch migration (in vitro).
Gene References into Functions
  1. These findings suggested that ERCC6L, which is highly expressed in breast cancer, acts as an oncogene, is involved in breast cancer development and may serve as a novel molecular target for the treatment of breast cancer. PMID: 30066865
  2. ERCC6L may stimulates cancer cell proliferation by promoting cell cycle through a way of RAB31-MAPK-CDK2, and it could be a potential biomarker for cancer prognosis and target for cancer treatment. PMID: 28178669
  3. Data indicate BEND3 as a new interaction partner for PICH in mitosis, and have defined the residues within a TPR-BEN domain interface that mediate this interaction. PMID: 28977671
  4. Characterization of the NTPR and BD1 interacting domains of the human PICH-BEND3 complex has been reported. PMID: 27487930
  5. the mitotic roles of PICH and explore further the role of PICH in the timely segregation of the ribosomal DNA locus, are reported. PMID: 27565185
  6. a novel SUMO-dependent regulation of PICH's function on mitotic centromeres, is reported. PMID: 27230136
  7. PICH and Topo II cooperate to prevent chromosome missegregation events in mitosis. PMID: 26643143
  8. PICH is a SUMO-interacting protein and a mitotic SUMO substrate. PMID: 25564610
  9. PICH recognizes and stabilizes DNA under tension during anaphase, thereby facilitating the resolution of entangled sister chromatids. PMID: 23973328
  10. PICH protein was required for the correct recruitment to the centromere of active topoisomerase IIalpha, an enzyme specialized in the catenation/decatenation process. PMID: 22563370
  11. PICH binds to BLM and enables BLM localization to anaphase centromeric threads. PICH and BLM unravel centromeric chromatin and keep anaphase DNA threads mostly free of nucleosomes. PMID: 21743438
  12. the PICH-Plk1 complex plays a critical role in maintaining prometaphase chromosome architecture PMID: 20130082
  13. the spindle checkpoint failure formerly attributed to the depletion of PICH most likely reflects an off-target effect that causes the lowering of Mad2 transcript and protein. PMID: 19904549
  14. These data identify PICH as a novel essential component of checkpoint signaling. PMID: 17218258
  15. The sensitivity to depletion of topo IIalpha might be linked to structural alterations within the centromere domain, indicated by shortening of the distance across metaphase sister centromeres and persistence of PICH-coated connections. PMID: 17956945
  16. PICH phosphorylation and its ATPase activity are required for mitotic chromosome compaction through the targeting of Plk1 to chromosome arms. PMID: 18418076
  17. Functional characterization of the mouse Ercc6l ortholog. PMID: 15917148

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Subcellular Location
Chromosome, centromere. Chromosome, centromere, kinetochore. Chromosome.
Protein Families
SNF2/RAD54 helicase family
Database Links

HGNC: 20794

OMIM: 300687

KEGG: hsa:54821

STRING: 9606.ENSP00000334675

UniGene: Hs.47558

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