Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

DFFB

Uniprot No.

O76075

Research Area

Cancer

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-338aa

Target Protein Sequence

MLQKPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQGYVSDIRRFLSAFHEPQVGLIQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTVGAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNLDHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTHKLNCDPSRIYKPQTRLKRKQPVRKRQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

45.1 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

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■ Q&A

Could you please tell me if the blot that you show for full length DFFB is a Western blot or a coomassie gel?
Is this just Ni-NTA purified protein or do you also use other methods? Is the DFFB expressed alone in ecoli or is it coexpessed with DFFA.

It is a coomassie gel.
The target protein was purified by Ni-NTA, and DFFB was expressed in Escherichia coli alone.

Could you please tell if the blot that you show for full lenght DFFB is a Western blot or a coomassie gel? Could you tell me exactly the amino acid sequence oft he tagged DFFB (where/which tag did you use). Is this just Ni-NTA purified protein or do you also use other methods. Is the DFFB expressed alone in ecoli or is it coexpessed with DFFA?

The blot showing full lenght DFFB is a coomassie gel. It is a Ni-NTA purified protein and the DFFB was expressed independently in Escherichia coli.

Target Background

Function

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Gene References into Functions

we show that executioner caspase activation of the apoptotic nuclease CAD/DFF40 is essential for TRAIL-induced mutations in surviving cells. As exposure to chemotherapy drugs also activates apoptotic caspases and presumably CAD, we hypothesized that these pathways may also contribute to the mutagenesis induced by conventional chemotherapy drugs, perhaps augmenting the mutations that arise from direct DNA damage PMID: 28981092
Dff40 expression is upregulated in atherosclerotic plaque. PMID: 28007744
the low expression levels of DFF40/CAD and the absence of DNA laddering as common molecular traits in glioblastoma PMID: 26755073
Data suggest DFF40 expression in breast cancer cell line is involved in drug sensitivity/resistance to doxorubicin; apoptotic cell death due to doxorubicin (a topoisomerase II inhibitor) is enhanced by DFF40 overexpression in breast cancer cell line. PMID: 26529233
Combinatorial use of some sulfonamides such as acetazolamide along with increased expression of DFF40 can potently kill tumor cells via apoptosis. PMID: 25086620
DFF40/CAD-independent mechanism driving conformational nuclear changes during caspase-dependent cell death PMID: 24838313
the highest order of chromatin compaction observed in the later steps of caspase-dependent apoptosis relies on DFF40/CAD-mediated DNA damage by generating 3'-OH ends in single-strand rather than double-strand DNA nicks/breaks PMID: 23430749
These results suggest a cooperative activity between CAD and DNAS1L3 to accomplish internucleosomal DNA fragmentation . PMID: 23229555
Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40 PMID: 22609799
During apoptotic rearrangement of interchromatin granule clusters, the nuclear matrix (NuMa rearrangement) and chromatin are closely associated. This process occurs in defined stages and depends on the activity of protein phosphatases, caspases and CAD. PMID: 22023725
the cytosolic levels of DFF40/CAD are determinants in achieving a complete apoptotic phenotype, including oligonucleosomal DNA degradation. PMID: 22253444
Data show that among the 13 SNPs in the 3 genes, only 3 were found to be polymorphic: R196K and K277R in the DFFB gene, and S12L in the EndoG gene, and all 6 SNPs in the FEN-1 gene were entirely monoallelic. PMID: 22011247
These data suggest that DFF 40 mediated apoptosis plays a significant role in mediating sepsis induced cellular dysfunction. PMID: 21820410
Mutations and aberrantly spliced transcripts for the CAD gene are frequently associated with hepatocellular carcinoma. PMID: 12610505
Hsp70 binds free CAD in TCR-stimulated T cells to stabilize and augment its activity. PMID: 12738667
CAD involves unrestricted accessibility of chromosomal DNA at the initial phase of apoptosis, followed by its nuclear immobilization that may prevent the release of the active nuclease into the extracellular environment. PMID: 15569712
PARP-1 poly(ADP-ribosyl)ation is a terminal event in the apoptotic response that occurs in response to DNA fragmentation and directly influences DFF40 activity PMID: 15703174
Interactions identified here between human placenta histone H1 carboxyl-terminal domain and DFF40/CAD target and activate linker DNA cleavage during the terminal stages of apoptosis. PMID: 15910001
Our findings of high frequency of Alu-mediated hCAD deletion in human hepatoma underscore the implication of hCAD in hepatocarcinogenesis PMID: 16007181
AIF is responsible for stage I nuclear morphology and HMW DNA degradation is a caspase-activated DNase and AIF-independent process PMID: 16049016
levels of CAD were significantly higher in the nuclear fraction of temporal lobe epilepsy samples PMID: 16121124
DFFB haploinsufficiency from 1p allelic loss is a contributing factor in oligodendroglioma development PMID: 16156899
in apoptotic cells, endogenous and exogenous CAD forms limited oligomers, representing the active nuclease PMID: 16204257
the N-terminal region was found to be responsible for the requirement of salt for fibril formation PMID: 16428311
CAD is downregulated at the mRNA and protein level during the erythroid differentiation in TF-1 cells. PMID: 16529748
poly-glutamic acid and heparin inhibit DFF40/CAD, the latter one being highly effective at nanomolar concentrations. The inhibitory poly-anions bind to the nuclease and impair its ability to bind double-stranded DNA. PMID: 16699957
These results suggest that CAD is the endogenous endonuclease that mediates internucleosomal DNA degradation in rotenone-induced apoptosis. PMID: 17239993
Data suggest that erythroblast chromatin degradation may involve caspase activated DNase and apoptosis inducing factor, enzymes distinct from those active in apoptotic cells. PMID: 17492772
changes induced in DNA conformation upon HMG-box binding makes the substrate more accessible to cleavage by DFF40/CAD nuclease and thus may contribute to preferential linker DNA cleavage during apoptosis PMID: 18239742
We have found that neither single-stranded DNA, single-stranded RNA, double-stranded RNA nor RNA-DNA heteroduplexes are cleaved by the DFF40/CAD nuclease. PMID: 18283539

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Subcellular Location

Cytoplasm. Nucleus.

Database Links

HGNC: 2773

OMIM: 601883

KEGG: hsa:1677

STRING: 9606.ENSP00000367454

UniGene: Hs.133089

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Code	CSB-EP006738HU
Abbreviation	Recombinant Human DFFB protein
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human DNA fragmentation factor subunit beta (DFFB)

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