Recombinant Human DNA repair protein XRCC4(XRCC4)

Code CSB-EP614413HU
Size US$2466
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names XRCC4
Uniprot No. Q13426
Research Area others
Alternative Names DNA double strand break repair and V(D)J recombination protein XRCC4; DNA repair protein XRCC4; SSMED; X ray repair complementing defective repair in Chinese hamster cells 4; X ray repair cross complementing 4; X ray repair cross complementing protein 4; X-ray repair cross-complementing protein 4; XRCC 4; XRCC4; XRCC4_HUMAN
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-336aa
Target Protein Sequence MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 65.3kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Gene References into Functions
  1. These data imply that the level of XRCC4 expression could be used to predict the effects of apoptosis-inducing drugs in cancer treatment. PMID: 29233683
  2. Three-locus model of gene-gene interactions OGG1 (rs1052133) * ADPRT (rs1136410) * XRCC4 (rs6869366) was associated with high genotoxic risk in coal miners. PMID: 28992182
  3. Variant rs3734091 was found to be significantly associated with breast cancer while rs6869366 variant did not show any association. These SNPs may influence the susceptibility of individuals to breast cancer in this Indian population. PMID: 29452234
  4. Results showed that eNOS and XRCC4 VNTR variants might play a potential role in schizophrenia + nicotine dependence and/or nicotine dependence pathophysiology. PMID: 29050484
  5. Phospho-blocking and -mimicking mutations impact both the stability and DNA bridging capacity of XRCC4/XLF complexes, but without affecting their ability to stimulate LIG4 activity. Implicit in this finding is that phosphorylation may regulate DNA bridging by XRCC4/XLF filaments. PMID: 28500754
  6. This study demonstrated both ligase IV and XRCC4 may act in concert to modulate the development of glioma. PMID: 27508978
  7. Data suggest that genetic variants of XRCC4 and ERCC1 may independently or jointly affect survival in chemotherapy-treated gastric cancer (GCa) patients by modulating the gene expression in the tumors. PMID: 28796378
  8. In a recombinant PNKP-XRCC4-LigIV complex, stable binding of PNKP requires XRCC4 phosphorylation. Only one PNKP protomer binds per XRCC4 dimer. Both the PNKP FHA and catalytic domains contact the XRCC4 coiled-coil and LigIV BRCT repeats. Multipoint contacts between PNKP and XRCC4-LigIV regulate PNKP recruitment and activity within NHEJ. PMID: 28453785
  9. Data suggest that stimulation of Artemis nuclease/DCLRE1C activity by XRCC4-DNA ligase IV hetero-complex and efficiency of blunt-end ligation are determined by structural configurations at the DNA ends. (XRCC4 = X-ray repair cross complementing 4) PMID: 28696258
  10. the various XRCC4 mutations that lead to primordial dwarfism and their impact on non-homologous end joining and V(D)J recombination are discussed (Review) PMID: 27169690
  11. XRCC4 expression might have an influence on results of radiotherapy for patients with esophageal squamous cell carcinoma. PMID: 27338590
  12. uterine cervical cancer patients with high Ku86 and XRCC4 expression had a significantly lower 5-year metastasis-free rate than others. PMID: 26867665
  13. Frequencies of XRCC4-1394 T/G+G/G genotypes were higher in patients (%34) than the controls (%18.7). The statistical analysis revealed that the individuals who had XRCC4-1394 T/G+G/G genotype had an increased risk for autism spectrum disorder (OR = 2.23, 95% CI = 1.10-4.55) PMID: 27064873
  14. Genetic Variations in XRCC4 (rs1805377) is not Associated with Hepatocellular Carcinoma in Thai Patients with Hepatitis B Virus Infection. PMID: 26925648
  15. These results suggested potential usefulness of the phosphorylation status of XRCC4 Ser320 as an indicator of DNA-PK functionality in living cells. PMID: 26666690
  16. Association between insertion/deletion polymorphism in intron 3 of XRCC4 and susceptibility to type I bipolar disorder PMID: 26484731
  17. For XRCC4 (rs1805377) polymorphism, no difference was found in distribution between the ESCC and control groups. PMID: 26166223
  18. using dual- and quadruple-trap optical tweezers combined with fluorescence microscopy, we show how human XRCC4, XLF and XRCC4-XLF complexes interact with DNA in real time PMID: 27437582
  19. The ends are then closely aligned, which requires XLF, a non-catalytic function of XRCC4-LIG4, and DNA-PK activity PMID: 26990988
  20. Polymorphisms of XRCC4 are associated with susceptibility to Colorectal Cancer. PMID: 25662981
  21. FBXW7 facilitates nonhomologous end-joining via K63-linked polyubiquitylation of XRCC4 in tumor cells. PMID: 26774286
  22. The XRCC4 -1394T/G polymorphism is associated with susceptibility to endometriosis in an Iranian population. PMID: 26088159
  23. Our results suggest that the XRCC4 rs2075685 polymorphism could influence the susceptibility to pancreatic cancer in a Chinese population. PMID: 26345895
  24. The TMPRSS2-ERG Gene Fusion Blocks XRCC4-Mediated Nonhomologous End-Joining Repair and Radiosensitizes Prostate Cancer Cells to PARP Inhibition PMID: 26026052
  25. Point mutations in XRCC4 is associated with microcephaly, short stature and genomic instability. PMID: 25839420
  26. Genetic susceptibility to schizophrenia was found in patients with genetic polymorphisms in intron 3 of the XRCC4 gene. PMID: 26112447
  27. our results suggest that XRCC4 rs2075685 polymorphism plays an important role in the risk of pancreatic cancer in a Chinese population PMID: 26045837
  28. Nonsense mutations in human XRCC4 have recently been associated with primordial dwarfism and, in our cases, with adult-onset neurological impairment, suggesting an important role for DNA repair in the brain. PMID: 25872942
  29. Polymorphisms of insertion/deletion at the intron 3 of the XRCC4 is associated with gastric cancer. PMID: 25527410
  30. Variants of the DNA damage repair gene XRCC4 increase the risk of esophageal cancer PMID: 25612937
  31. no association was found between variants of XRCC4 rs2075685 and XRCC4 rs1805377 and development of glioma PMID: 25973104
  32. study identified a novel pathogenic variant in XRCC4; finding expands the spectrum of DNA damage repair syndromes to include XRCC4 deficiency causing severe postnatal growth failure, microcephaly, gonadal failure, metabolic syndrome, and possibly tumor predisposition PMID: 25742519
  33. Our data showed that Cd altered the phosphorylation of DNA-PKcs, and reduced the expression of both XRCC4 and Ligase IV in irradiated cells. PMID: 26201248
  34. our findings document the role of XRCC4 in non-BRCA1/2 breast cancer PMID: 25360583
  35. the intron 3 VNTR polymorphism in the XRCC4 gene may be associated with the etiopathogenesis of RA as a marker of immune aging. PMID: 25494482
  36. The present results collectively indicated that Lys271, but not Lys210, of XRCC4 is required for the nuclear localization of XRCC4 and LIG4 and that the nuclear localizing ability is essential for DSB repair function of XRCC4. PMID: 25934149
  37. The XRCC4 promoter -652G>T polymorphism is functional and may influence genetic susceptibility to prostate cancer. PMID: 25096509
  38. We present comprehensive genetic and cellular evidence that mutations in XRCC4 cause microcephalic primordial dwarfism. PMID: 25728776
  39. XRCC4 C-terminal point mutants, R325F and N326L, are functionally deficient in terms of cell survival after irradiation. PMID: 25597996
  40. There was longer survival among patients with non-invasive tumours associated with DNA repair X-ray repair cross-complementing protein 4 (XRCC4) heterozygous genotype compared with wild-type. PMID: 24666523
  41. Phosphorylation of XRCC4 in mitosis was not responsible for its failure to localize to mitotic chromosomes and phosphorylation did not affect the interaction of XRCC4 with DNA ligase IV PMID: 25165869
  42. Taken together, our results indicate that XRCC4 is required not only for the promotion of NHEJ during interphase but also for its M-phase-specific suppression of DSB repair. PMID: 25166505
  43. XRCC4 codon 247 polymorphism may be associated with DIA risk and prognosis among the Guangxi population. PMID: 24378850
  44. Results show that in cells deficient for Lig4, chromosomal translocations junctions had significantly longer deletions and more microhomologies. PMID: 25201414
  45. Characterization of the protein interaction domains that modulate the XRCC4/Ligase IV interaction. PMID: 23794378
  46. Single nucleotide polymorphisms in XRCC4 were associated with breast cancer risk. PMID: 24062231
  47. These results indicate for the first time that DNA ligase IV (LIG4) rs1805388 and X-ray Repair Cross Complementing-4 (XRCC4) rs1805377, alone or in combination, are associated with a risk of gliomas. PMID: 23663450
  48. The induced DNA damage by Methyl Methanesulfonate increases in Chronic Obstructive Pulmonary Disease patients with variant genotypes in OGG1 (Ser326Cys) showing impairment of DNA repair. PMID: 24053728
  49. XRCC4P may be a genetic modifier for the risk and outcome of hepatocellular carcinoma induced by aflatoxin B1 exposure. PMID: 23788213
  50. The chromatin binding of XRCC4 was dependent on the presence of LIG4. PMID: 23994631

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Involvement in disease Short stature, microcephaly, and endocrine dysfunction (SSMED)
Subcellular Location Nucleus
Protein Families XRCC4 family
Tissue Specificity Widely expressed.
Database Links

HGNC: 12831

OMIM: 194363

KEGG: hsa:7518

STRING: 9606.ENSP00000342011

UniGene: Hs.567359

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