Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

DPYSL5

Uniprot No.

Q9BPU6

Research Area

Neuroscience

Alternative Names

Collapsin response mediator protein 5; CRAM; CRMP 3 associated molecule; CRMP 5; CRMP3 associated molecule; Dihydropyrimidinase like 5; Dihydropyrimidinase related protein 5; Dihydropyrimidinase-related protein 5; DPYL5_HUMAN; DPYS L5; DPYSL 5; DPYSL5; DRP 5; DRP5; FLJ45383; Ulip 6; Ulip6; ULIP6 protein; UNC33-like phosphoprotein 6

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-564aa

Target Protein Sequence

MLANSASVRILIKGGKVVNDDCTHEADVYIENGIIQQVGRELMIPGGAKVIDATGKLVIPGGIDTSTHFHQTFMNATCVDDFYHGTKAALVGGTTMIIGHVLPDKETSLVDAYEKCRGLADPKVCCDYALHVGITWWAPKVKAEMETLVREKGVNSFQMFMTYKDLYMLRDSELYQVLHACKDIGAIARVHAENGELVAEGAKEALDLGITGPEGIEISRPEELEAEATHRVITIANRTHCPIYLVNVSSISAGDVIAAAKMQGKVVLAETTTAHATLTGLHYYHQDWSHAAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSGVQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADADVVVWDPEATKTISASTQVQGGDFNLYENMRCHGVPLVTISRGRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRTPYLGDVAVVVHPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPKRASARILAPPGGRSSGIW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

66.4 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human DPYSL5 was expressed with the amino acid range of 1-564. The calculated molecular weight for this DPYSL5 protein is 66.4 kDa. This DPYSL5 protein is produced using e.coli expression system. The N-terminal 10xHis tag and C-terminal Myc tag was fused into the coding gene segment of DPYSL5, making it easier to detect and purify the DPYSL5 recombinant protein in the later stages of expression and purification.

Human dihydropyrimidinase-related protein 5 (DPYSL5) functions as a key player in various cellular processes, notably in cellular signaling and differentiation. Its primary role involves the regulation of cell growth and differentiation, contributing to the intricate balance of cellular physiology. DPYSL5's involvement in signaling pathways makes it a subject of interest across diverse research areas. In cancer research, DPYSL5 may be explored as a potential biomarker or therapeutic target, unraveling its impact on cell proliferation and invasion. Additionally, its relevance in neurobiology could be investigated to understand its role in neuronal function and potential implications in neurodegenerative disorders.

Target Background

Function

May have a function in neuronal differentiation and/or axon growth.

Gene References into Functions

Study describes the relevance of DRP5 during osteosarcoma development. DRP5 was upregulated in osteosarcoma specimens and cell lines and shown to function via the downstream MMPs. Inhibition of DRP5 suppressed the growth of cancer cells in vitro and in vivo, and high expression levels of DRP5 were associated with poor prognosis in osteosarcoma patients. PMID: 28374915
Our findings suggest that CRMP5 serves as a major mediator of Notch signaling and Akt activation by controlling the degradation of the Notch receptor, with implications for defining a biomarker signature in glioblastoma PMID: 26122847
present study suggested that CRAM could be a clinical prognostic marker for patients with cervical cancer PMID: 24695578
study elucidates a novel regulatory mechanism that utilizes CRMP5-induced mitophagy to orchestrate proper dendrite outgrowth and neuronal function. PMID: 24324268
identified residues that are crucial for determining the preference for hetero-oligomer or homo-oligomer formation. In spite of being the CRMP family member most closely related to dihydropyrimidinase, CRMP-5 does not have amidohydrolase activity. PMID: 23373749
New CRMP5 isoform present in the nucleus is associated with Glioma. PMID: 23298946
CRMP-5-IgG defines a paraneoplastic ophthalmological entity of combined optic neuritis and retinitis with vitreous inflammatory cells. PMID: 12838519
CRMP-5 autoimmune myelopathy and occult neoplasia are important considerations in patients with insidiously progressive myelopathy, especially with known cancer risk. PMID: 18306241
We describe a patient with optic neuropathy and vitritis as the only clinical manifestations of paraneoplastic optic neuropathy secondary to lung cancer marked by an extremely high titer of CRMP-5 antibody. PMID: 18347453
findings point at CRMP5 as a novel marker for distinguishing between highly aggressive neuroendocrine carcinoma and the other lung cancers. PMID: 18769332
patients with CV2/CRMP5-Ab and thymoma developed myasthenic syndrome more frequently PMID: 18931014

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family

Database Links

HGNC: 20637

OMIM: 608383

KEGG: hsa:56896

STRING: 9606.ENSP00000288699

UniGene: Hs.299315

Code	CSB-EP857765HU
Abbreviation	Recombinant Human DPYSL5 protein
MSDS	MSDS Datasheet
Size	US$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Dihydropyrimidinase-related protein 5 (DPYSL5)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage