Recombinant Human Dihydropyrimidinase-related protein 5 (DPYSL5)

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Code CSB-EP857765HU
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Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
DPYSL5
Uniprot No.
Research Area
Neuroscience
Alternative Names
Collapsin response mediator protein 5; CRAM; CRMP 3 associated molecule; CRMP 5; CRMP3 associated molecule; Dihydropyrimidinase like 5; Dihydropyrimidinase related protein 5; Dihydropyrimidinase-related protein 5; DPYL5_HUMAN; DPYS L5; DPYSL 5; DPYSL5; DRP 5; DRP5; FLJ45383; Ulip 6; Ulip6; ULIP6 protein; UNC33-like phosphoprotein 6
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-564aa
Target Protein Sequence
MLANSASVRILIKGGKVVNDDCTHEADVYIENGIIQQVGRELMIPGGAKVIDATGKLVIPGGIDTSTHFHQTFMNATCVDDFYHGTKAALVGGTTMIIGHVLPDKETSLVDAYEKCRGLADPKVCCDYALHVGITWWAPKVKAEMETLVREKGVNSFQMFMTYKDLYMLRDSELYQVLHACKDIGAIARVHAENGELVAEGAKEALDLGITGPEGIEISRPEELEAEATHRVITIANRTHCPIYLVNVSSISAGDVIAAAKMQGKVVLAETTTAHATLTGLHYYHQDWSHAAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSGVQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADADVVVWDPEATKTISASTQVQGGDFNLYENMRCHGVPLVTISRGRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRTPYLGDVAVVVHPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPKRASARILAPPGGRSSGIW
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
66.4 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The recombinant Human DPYSL5 was expressed with the amino acid range of 1-564. The calculated molecular weight for this DPYSL5 protein is 66.4 kDa. This DPYSL5 protein is produced using e.coli expression system. The N-terminal 10xHis tag and C-terminal Myc tag was fused into the coding gene segment of DPYSL5, making it easier to detect and purify the DPYSL5 recombinant protein in the later stages of expression and purification.

Human dihydropyrimidinase-related protein 5 (DPYSL5) functions as a key player in various cellular processes, notably in cellular signaling and differentiation. Its primary role involves the regulation of cell growth and differentiation, contributing to the intricate balance of cellular physiology. DPYSL5's involvement in signaling pathways makes it a subject of interest across diverse research areas. In cancer research, DPYSL5 may be explored as a potential biomarker or therapeutic target, unraveling its impact on cell proliferation and invasion. Additionally, its relevance in neurobiology could be investigated to understand its role in neuronal function and potential implications in neurodegenerative disorders.

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Target Background

Function
May have a function in neuronal differentiation and/or axon growth.
Gene References into Functions
  1. Study describes the relevance of DRP5 during osteosarcoma development. DRP5 was upregulated in osteosarcoma specimens and cell lines and shown to function via the downstream MMPs. Inhibition of DRP5 suppressed the growth of cancer cells in vitro and in vivo, and high expression levels of DRP5 were associated with poor prognosis in osteosarcoma patients. PMID: 28374915
  2. Our findings suggest that CRMP5 serves as a major mediator of Notch signaling and Akt activation by controlling the degradation of the Notch receptor, with implications for defining a biomarker signature in glioblastoma PMID: 26122847
  3. present study suggested that CRAM could be a clinical prognostic marker for patients with cervical cancer PMID: 24695578
  4. study elucidates a novel regulatory mechanism that utilizes CRMP5-induced mitophagy to orchestrate proper dendrite outgrowth and neuronal function. PMID: 24324268
  5. identified residues that are crucial for determining the preference for hetero-oligomer or homo-oligomer formation. In spite of being the CRMP family member most closely related to dihydropyrimidinase, CRMP-5 does not have amidohydrolase activity. PMID: 23373749
  6. New CRMP5 isoform present in the nucleus is associated with Glioma. PMID: 23298946
  7. CRMP-5-IgG defines a paraneoplastic ophthalmological entity of combined optic neuritis and retinitis with vitreous inflammatory cells. PMID: 12838519
  8. CRMP-5 autoimmune myelopathy and occult neoplasia are important considerations in patients with insidiously progressive myelopathy, especially with known cancer risk. PMID: 18306241
  9. We describe a patient with optic neuropathy and vitritis as the only clinical manifestations of paraneoplastic optic neuropathy secondary to lung cancer marked by an extremely high titer of CRMP-5 antibody. PMID: 18347453
  10. findings point at CRMP5 as a novel marker for distinguishing between highly aggressive neuroendocrine carcinoma and the other lung cancers. PMID: 18769332
  11. patients with CV2/CRMP5-Ab and thymoma developed myasthenic syndrome more frequently PMID: 18931014

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Subcellular Location
Cytoplasm.
Protein Families
Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family
Database Links

HGNC: 20637

OMIM: 608383

KEGG: hsa:56896

STRING: 9606.ENSP00000288699

UniGene: Hs.299315

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