Name: Recombinant Human Dihydropyrimidinase (DPYS)
Brand: CUSABIO
SKU: 007169HU

Product Details

Purity

>85% (SDS-PAGE)

Target Names

DPYS

Uniprot No.

Q14117

Alternative Names

DHP; DHPase; Dihydropyrimidinase; Dihydropyrimidine amidohydrolase; Dpys; DPYS_HUMAN; Hydantoinase

Species

Homo sapiens (Human)

Expression Region

1-519

Target Protein Sequence

MAAPSRLLIR GGRVVNDDFS EVADVLVEDG VVRALGHDLL PPGGAPAGLR VLDAAGKLVL PGGIDTHTHM QFPFMGSRSI DDFHQGTKAA LSGGTTMIID FAIPQKGGSL IEAFETWRSW ADPKVCCDYS LHVAVTWWSD QVKEEMKILV QDKGVNSFKM FMAYKDLYMV TDLELYEAFS RCKEIGAIAQ VHAENGDLIA EGAKKMLALG ITGPEGHELC RPEAVEAEAT LRAITIASAV NCPLYIVHVM SKSAAKVIAD ARRDGKVVYG EPIAASLGTD GTHYWNKEWH HAAHHVMGPP LRPDPSTPDF LMNLLANDDL TTTGTDNCTF NTCQKALGKD DFTKIPNGVN GVEDRMSVIW EKGVHSGKMD ENRFVAVTST NAAKIFNLYP RKGRIAVGSD ADIVIWDPKG TRTISAKTHH QAVNFNIFEG MVCHGVPLVT ISRGKVVYEA GVFSVTAGDG KFIPRKPFAE YIYKRIKQRD RTCTPTPVER APYKGEVATL KSRVTKEDAT AGTRKQAHP

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

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Customer Reviews and Q&A

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■ Q&A

Do you have data on the biological activity, specificity, stability and purity for the following product:
https://www.cusabio.com/Recombinant-Protein/Recombinant-Human-DihydropyrimidinaseDPYS-Escherichia-Coli-11918250.html
Do you know, if there is any difference in biological activity for this recombinant protein between Expression in e.coli or mammalian System?

Thanks for your inquiry.
Recombinant Human Dihydropyrimidinase(DPYS)
Express Region:1-519aa; Full length protein.
Tag information:EP,BP,MP:N-terminal 10xHis-tagged and C-terminal Myc-tagged；YP:N-terminal 6xHis-tagged
As to the activity, we haven't detect it, so we can't gurantee. The protein we produce is the full length protein, and it's purify under the mild condition. The protein has post-translational modifications and the eukaryotic expression system is more likely to be active. MP is closer to the natural state.
For the purity, our QC standard is 85% through SDS-PAGE.

Regarding the purity of 85%, I would like to know, which kind of impurities the remaining 15% are? Are these residues of the solvent or buffer or salts or other proteins?
Would a further purification by ion exchange and size exclusion chromatography be possible?
Do you have any information on the biological activity?

Thanks for your inquiry. The other 15% are the other proteins.
We could try the further purification. The standard can be 90%. During the further purification, it's possible the further purification failed, or the big lose of the protein. We can't guarantee. If we success, the cost will be $83, and the lead time will be 3-5 working days longer.
If we failed, we will also provide the protein with 85% purity.
For the activity, we haven't test it.

Target Background

Function

Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.

Gene References into Functions

4 newly identified DHP deficient patients presented with strongly elevated levels of 5,6-dihydrouracil and 5,6-dihydrothymine in urine and a highly variable clinical presentation, ranging from asymptomatic to infantile spasm and reduced white matter and brain atrophy. Analysis of the DHP gene (DPYS) showed the presence of 8 variants including 4 novel/rare missense variants and one novel deletion. PMID: 29054612
Two unrelated pediatric DPYS deficiency cases are being described as compound heterozygotes for a novel intronic mutation c.1443+5G>A in intron 8 and a previously described missense mutation c.1001A>G (p.Q334R) in exon 6. PMID: 26771602
Stepwise Cox regression modelling suggested that the methylation of genes HSPB1, CCND2 and DPYS contributed objective prognostic information to Gleason score and PSA with respect to prostate cancer-related death. PMID: 25193387
The p.S379R and p.L7V mutations were likely to cause structural destabilization and protein misfolding. Four mutations were identified in multiple unrelated DHP patients, indicating that DHP deficiency may be more common than anticipated. PMID: 20362666
Results indicate that missense and nonsense variants in DPYS are infrequent, however, the development of serious primarily gastrointestinal toxicity could be influenced by non-coding DPYS sequence variants c.-1T>C and IVS1-58T>C. PMID: 19649633
clinical, biochemical & genetic findings of two newly identified patients with a complete DHP deficiency; both patients were compound heterozygous for the missense mutation 1078T>C (W360R) in exon 6 and a novel missense mutation 1235G>T (R412M) in exon 7 PMID: 17383919
data presented in this study offers evidence for the possible genetic regulation of the DPYS gene and its possible influence on uracil catabolic pathway PMID: 18075467

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Involvement in disease

Dihydropyrimidinase deficiency (DPYSD)

Protein Families

Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family

Tissue Specificity

Liver and kidney.

Database Links

HGNC: 3013

OMIM: 222748

KEGG: hsa:1807

STRING: 9606.ENSP00000276651

UniGene: Hs.443161

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Code	CSB-YP007169HU
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Code	CSB-EP007169HU-B
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Code	CSB-MP007169HU
MSDS	MSDS Datasheet
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Source	Mammalian cell
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Recombinant Human Dihydropyrimidinase (DPYS)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

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