Recombinant Human E3 ubiquitin-protein ligase parkin(PRKN)

Very nice to receive your inquiry.
This protein is the inclusion bodies from E. coli, but we can guarantee that the protein will be provided in soluble form after refolding even if it's expressed in inclusion bodies form.
In theory, the final protein provided is properly folded. If you need the protein expressed in supernatant , we can try to optimize the condition, and conduct supernatant expression, but we still can't 100% guarantee.
Why do you focus on this question ? Do you want to know the activity of this protein or for some other reasons ? If it's just considered from the activity aspect, you can consider the eukaryotic expressed protein (the activity is more likely but not 100 % guaranteed to be active), we have already successfully expressed the BP and MP proteins before, you can consider to purchase eukaryotic expressed protein.
Sorry, we haven't removed the tag of these proteins before, neither for the EP protein.
Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order. We can try enzyme digestion, but we can't guarantee 100% successfully.
The overall success rate of enzyme digestion data analysis is 75%-86%.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.