PRKN is a gene that encodes the protein parkin. Parkin, also commonly known as E3 ubiquitin - protein ligase parkin, is a crucial enzyme in the ubiquitin - proteasome system. It functions by tagging target proteins with ubiquitin, marking them for degradation. It is involved in the PI3K - Akt and MAPK signaling pathways.
Mutations in PRKN are strongly linked to autosomal recessive early - onset Parkinson's disease. Regarding drug development, researchers are exploring small molecules that can enhance parkin's activity. These efforts aim to correct the abnormal protein degradation caused by parkin deficiency, offering potential new treatments for related neurodegenerative disorders.
Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) (CSB-BP017451HU)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
PRKN Recombinant Monoclonal Antibody (CSB-RA199844A0HU)
Validated Data
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30µg), U-251MG whole cell lysate(30µg), Jurkat whole cell lysate(30µg), K562 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), HEK293T whole cell lysate(30µg), SW620 whole cell lysate(30µg)
All lanes: Parkin antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 30 kDa
Exposure time: 2min
Immunofluorescence staining of A-549 cell with CSB-RA199844A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing 786-O cells stained with CSB-RA199844A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
PRKN Antibody (CSB-PA017451LA01HU)
Validated Data
Western Blot
Positive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate
All lanes: PRKN antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 49, 24, 31, 43, 36, 44, 47 kDa
Observed band size: 52 kDa
IHC image of CSB-PA017451LA01HU diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA017451LA01HU diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of PC-3 cells using CSB-PA017451LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
The following PRKN reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
PRKN Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA017451LA01HU | PRKN Antibody | Human | ELISA, WB, IHC, IF |
CSB-PA076233 | PARK2 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA003708 | PARK2 Antibody | Human | WB, IHC, ELISA |
CSB-PA020235 | Phospho-PARK2 (S131) Antibody | Human | WB, IHC, ELISA |
CSB-PA020236 | PARK2 Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-RA199844A0HU | PRKN Recombinant Monoclonal Antibody | Human | ELISA, WB, IF, FC |
PRKN Antibodies for Rattus norvegicus (Rat)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA864348ZA01RA | Prkn Antibody | Rattus norvegicus | ELISA, WB (ensure identification of antigen) |
PRKN Antibodies for Mus musculus (Mouse)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA893911ZA01MO | Prkn Antibody | Mus musculus | ELISA, WB (ensure identification of antigen) |
PRKN Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP017451HU CSB-MP017451HU CSB-EP017451HU-B |
Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) | Yeast Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP017451HU | Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) | E.coli |
CSB-BP017451HU | Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) | Baculovirus |
CSB-BP017451HUb1 | Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) | Baculovirus |
PRKN Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP864348RA CSB-EP864348RA CSB-MP864348RA CSB-EP864348RA-B |
Recombinant Rat E3 ubiquitin-protein ligase parkin (Park2) | Yeast E.coli Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP864348RAb1 | Recombinant Rat E3 ubiquitin-protein ligase parkin (Park2) | E.coli |
CSB-BP864348RA | Recombinant Rat E3 ubiquitin-protein ligase parkin (Prkn) | Baculovirus |
PRKN Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP893911MO CSB-BP893911MO CSB-MP893911MO CSB-EP893911MO-B |
Recombinant Mouse E3 ubiquitin-protein ligase parkin (Prkn) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
PRKN Proteins for Mus musculus(Mouse)
Code | Product Name | Source |
---|---|---|
CSB-EP893911MO | Recombinant Mouse E3 ubiquitin-protein ligase parkin (Prkn) | E.coli |