PARK2 Antibody

Datasheet
Code CSB-PA076233
Product Type Polyclonal Antibody
Size US$297
Uniprot No. O60260
Image
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using Parkin antibody.
  • Western blot analysis of extracts from Jukat cells, using Parkin antibody.
  • Western blot analysis of extracts from Jurkat cells using Parkin antibody. The lane on the left is treated with synthesized peptide.
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Synthesized peptide derived from Human Parkin antibody.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous levels of total Parkin protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100
Relevance Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform ofSNCAIP, SEPT5, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy. Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy), potentially by the ubiquitination of mitochondrial proteins. Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in the regulation of neuron death. Limits the production of reactive oxygen species (ROS). Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress. Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene.

Fang Yang, J. Biol. Chem., Apr 2005; 280: 17154 - 17162.
Yukiko Kuroda, Hum. Mol. Genet., Mar 2006; 15: 883 - 895.
Iris H. Henn, J. Neurosci., Feb 2007; 27: 1868 - 1878.
Monica Joch, Mol. Biol. Cell, Jun 2007; 10.1091/mbc.E05-11-1027.

Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality Polyclonal
Alias PARK2; PRKN; PRKN2; Parkinson disease protein 2; Parkinson juvenile disease protein 2
Immunogen Species Homo sapiens (Human)
Target Names PRKN
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2
Involvement in disease Parkinson disease (PARK); Parkinson disease 2 (PARK2)
Subcellular Location Cytoplasm, cytosol, Nucleus, Endoplasmic reticulum, Mitochondrion
Protein Families RBR family, Parkin subfamily
Tissue Specificity Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons fro
Database Links

HGNC: 8607

OMIM: 168600

KEGG: hsa:5071

STRING: 9606.ENSP00000355865

UniGene: Hs.132954

Pathway Protein processing in endoplasmic reticulum
Ubiquitin mediated proteolysis

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