Your Good Partner in Biology Research
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Your Good Partner in Biology Research
| Code | CSB-PA017451LA01HU |
| See More Details |  |
| Size |
US$299Purchase it in Cusabio online store (only available for customers from the US) |
| Uniprot No. | O60260 |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol |
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase parkin protein (1-465AA) |
| Raised in | Rabbit |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:500 |
| Relevance | Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy. Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by promoting the ubiquitination of mitochondrial proteins such as TOMM20, RHOT1/MIRO1 and USP30 (PubMed:24896179). Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in the regulation of neuron death. Limits the production of reactive oxygen species (ROS). Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress. Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene. |
| Form | Liquid |
| Conjugate | Non-conjugated |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method | >95%, Protein G purified |
| Isotype | IgG |
| Clonality | Polyclonal |
| Alias | E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.27) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2), PRKN, PARK2 |
| Immunogen Species | Human |
| Research Area | Neuroscience |
| Target Names | PRKN |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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| Function | Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2 |
| Involvement in disease | Parkinson disease (PARK); Parkinson disease 2 (PARK2) |
| Subcellular Location | Cytoplasm, cytosol, Nucleus, Endoplasmic reticulum, Mitochondrion |
| Protein Families | RBR family, Parkin subfamily |
| Tissue Specificity | Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons fro |
| Database Links |
HGNC: 8607 OMIM: 168600 KEGG: hsa:5071 STRING: 9606.ENSP00000355865 UniGene: Hs.132954 |
| Pathway |
Protein processing in endoplasmic reticulum
Ubiquitin mediated proteolysis |
PRKN Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA017451KA01HU | PARK2 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA076233 | PARK2 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA003708 | PARK2 Antibody | Human | WB,IHC,ELISA |
| CSB-PA020235 | Phospho-PARK2 (S131) Antibody | Human | WB,IHC,ELISA |
| CSB-PA020236 | PARK2 Antibody | Human,Mouse,Rat | WB,IHC,IF,ELISA |
PRKN Antibodies for Human
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA017451LB01HU | PRKN Antibody, HRP conjugated | Human | ELISA |
| CSB-PA017451LC01HU | PRKN Antibody, FITC conjugated | Human | ELISA |
| CSB-PA017451LD01HU | PRKN Antibody, Biotin conjugated | Human | ELISA |
PRKN Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-BP017451HUb1 | Recombinant Human E3 ubiquitin-protein ligase parkin(PRKN) | Baculovirus |
| CSB-YP017451HU CSB-MP017451HU |
Recombinant Human E3 ubiquitin-protein ligase parkin(PRKN) | Yeast Mammalian cell |
| CSB-EP017451HU | Recombinant Human E3 ubiquitin-protein ligase parkin(PRKN) | E.coli |
| CSB-BP017451HU | Recombinant Human E3 ubiquitin-protein ligase parkin(PRKN) | Baculovirus |
2-hydroxyisobutyryl-HIST1H2BC (K12) Antibody
Applications: ELISA, WB, ICC, IF
Species Reactivity: Human
