Recombinant Human Ficolin-3(FCN3)

In Stock
Code CSB-EP008552HUc7
Size US$2466
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names FCN3
Uniprot No. O75636
Research Area Immunology
Alternative Names FCN3; FCNH; HAKA1; Ficolin-3; Collagen/fibrinogen domain-containing lectin 3 p35; Collagen/fibrinogen domain-containing protein 3; Hakata antigen
Species Homo sapiens (Human)
Source E.coli
Expression Region 24-299aa
Target Protein Sequence QEHPSCPGPRELEASKVVLLPSCPGAPGSPGEKGAPGPQGPPGPPGKMGPKGEPGDPVNLLRCQEGPRNCRELLSQGATLSGWYHLCLPEGRALPVFCDMDTEGGGWLVFQRRQDGSVDFFRSWSSYRAGFGNQESEFWLGNENLHQLTLQGNWELRVELEDFNGNRTFAHYATFRLLGEVDHYQLALGKFSEGTAGDSLSLHSGRPFTTYDADHDSSNSNCAVIVHGAWWYASCYRSNLNGRYAVSEAAAHKYGIDWASGRGVGHPYRRVRMMLR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 34.4 kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota.
Gene References into Functions
  1. increased SFRP4 and ficolin-3 levels are significantly associated with gestational diabetes mellitus development and might be important risk factors for this pregnancy complication. PMID: 29491225
  2. Study found the ratio of ficolin-3/adiponectin at 16-18 weeks of gestation was changed in pregnant women who subsequently developed gestational diabetes mellitus GDM, and might provide effective early predicting and screening for GDM. PMID: 28445618
  3. Ficolin-3 was overexpressed in the serum of most hepatocellular carcinoma patients after Radiofrequency ablation . Ficolin-3 might be a biomarker for Radiofrequency ablation treatment efficacy and a potential target for hepatocellular carcinoma immunotherapy. PMID: 29386009
  4. Serum H-ficolin levels inversely correlated with ground-glass opacity score on chest computed tomography in systemic sclerosis-interstitial lung disease (SSc-ILD) patients. H-ficolin-related innate immunity may be involved in SSc-ILD development. PMID: 28432700
  5. Ficolin-3 plasma levels are associated with the presence and progression of abdominal aortic aneurysm (AAA), suggesting its potential role as a biomarker of AAA PMID: 28039962
  6. results suggest that anti-ficolin-3 antibodies could be useful for the diagnosis of active nephritis in SLE patients PMID: 27631981
  7. this study shows that H-ficolin may aid clearance of influenza A virus by promoting monocyte uptake of the virus, while reducing viral replication and virus-induced TNF-a responses in these cells PMID: 27856789
  8. These results suggest that polymorphisms in the FCN3 gene cooperate to increase ficolin-3 concentration and that it might contribute to leprosy susceptibility by favoring Mycobacterium leprae infection. PMID: 28241035
  9. subjects that were heterozygote carriers of both FCN2 + 6424 and FCN3 + 1637delC were sufficient mannan-binding lectin producers PMID: 26795763
  10. in patients with systemic lupus erythematosus, there was no significant association between ficolin-1 and ficolin-3 with lupus nephritis PMID: 27981461
  11. Monitoring serum H-ficolin levels was shown to be of no benefit in terms of predicting severe infection. PMID: 26377840
  12. Serum levels of ficolin-2 and ficolin-3 were significantly lower in the cardiac syndrome X patients compared to controls. PMID: 27312152
  13. LPS induces a tissue-specific recruitment of ficolin-3 and ficolin-1 in the lung and systemic compartment, respectively, suggesting an important role of distinct lectin complement pathway initiators in the local pulmonary and systemic host defence. PMID: 26868430
  14. Lower serum ficolin-3 levels were correlated with injury severity following traumatic brain injury. PMID: 26627059
  15. this study provide novel insight in the binding and complement activating capacity of the lectin pathway initiation molecules ficolin-2 and ficolin-3 towards relevant Gram-negative pathogens of pathophysiological relevance. PMID: 26074063
  16. Data indicate differences in the plasma concentrations of collectin liver 1 and collectin kidney 1, M-ficolin and H-ficol in systemic lupus erythematosus (SLE) patients compared to a group of healthy controls. PMID: 26154564
  17. H-ficolin participates in A. fumigatus defence through the activation of the lectin complement pathway, enhanced fungus-host interactions and modulated immune responses. PMID: 26133042
  18. data suggest that high levels of the complement activating molecule H-ficolin are associated with an increased risk of future progression to microalbuminuria in patients with newly diagnosed type 1 diabetes. PMID: 25064124
  19. High ficolin-3 level at the time of transplantation was an independent significant risk factor for shorter graft survival. PMID: 25222012
  20. There is lack of association of serum mannose-binding lectin or ficolins with complement activation in patients with antiphospholipid antibodies. PMID: 25083730
  21. This study aims to investigate whether an association exists between the ficolins that are part of the lectin complement pathway and systemic lupus erythematosus. PMID: 25069872
  22. Both ficolin-3 and MASP-2 levels correlated inversely with the time from the onset of the attack of hereditary angioedema until blood sampling PMID: 25042985
  23. Data show that the plasmid pETb-ficolin 3 was cloned successfully and the purity of the protein His-ficolin 3 was over 90%. PMID: 25001927
  24. Data show that the expression of ficolin-3(FCN3) in ovarian cancer (OC) was inversely correlated with serum ficolin-3 and lower in comparison with controls. PMID: 23744477
  25. low levels of ficolin-3 are associated with advanced heart failure and outcome. The decrease of ficolin-3 was associated with increased complement activation. PMID: 23596511
  26. Increased fucosylation of ficolin 3 in plasma of the rheumatoid arthritis patients PMID: 23107985
  27. Survival analyses showed that high pre-transplant serum levels of FCN3 were associated with decreased graft survival, suggesting an important role of FCN3 in the pathophysiology of kidney graft rejection PMID: 23416240
  28. Data indicate that the median concentration of MASP-2/ficolin-3 complexes was 119.7 AU/ml (range: 2.9-615.5 AU/ml). PMID: 23142462
  29. Both pre-term deliveries and low birth weight (independently of gestational age) were significantly associated with low H-ficolin concentrations but not with heterozygosity for the FCN3 1637delC frameshift mutation. PMID: 22226667
  30. Found that a common variant of FCN3/CD164L2 is associated with hypertension in Chinese population. PMID: 22471352
  31. H-ficolin has antiviral activity against influenza A virus (IAV). H-ficolin also fixes complement to a surface coated with IAV. PMID: 22851708
  32. The purpose of this study was to determine whether circulating levels of ficolin-2 and ficolin-3 are altered in normal pregnancy and pre-eclampsia. PMID: 22670778
  33. Cord blood MBL concentrations were significantly lower in intrauterine-growth-restriction (IUGR) cases than controls. No differences in H- and L-ficolin concentrations were observed between groups. PMID: 22082351
  34. in whole serum. MASP-2 was co-purified with H-ficolin, and the purified H-ficolin.MASP-2 complex could activate complement as measured by cleavage of complement factor C4 PMID: 22238349
  35. Data indicate that the deposition of both C4 and C3 showed a significant positive correlation with the serum concentration of Ficolin-3. PMID: 21085669
  36. MBL-associated serine protease-3 down-regulate Ficolin-3 mediated complement activation through the lectin pathway. PMID: 19939495
  37. Hakata antigen, a ficolin associated with MBL-associated serine proteases and small MBL-associated protein, activates the lectin complement pathway. PMID: 11907111
  38. Hakata circulates as Hakata-MASPs complex in the blood, binds Aerococcus viridans polysaccharide and inhibits A. viridans growth PMID: 12367778
  39. The X-ray structures of H-ficolin trimeric recognition domains, alone and in complex with various ligands, have been solved. PMID: 17215869
  40. variations in H-ficolin appear to be of limited importance in the pathogenesis of Crohn's disease PMID: 17303612
  41. Ficolin 3 mediates the clearance of late apoptotic cells, which suggests that this protein is involved in the maintenance of tissue homeostasis and might play a protective role against the development of autoimmunity. PMID: 17469142
  42. Ficolin-3 has a high complement activating potential and is the only collagenase proteolytic resistant molecule among the lectin complement pathway initiators. PMID: 18006063
  43. an FCN3+1637delC deletion variant disrupting the possibility for pattern recognition was detected; characterization of recombinant variant Ficolin-3 shows that homozygosity for the FCN3+1637delC deletion may lead to Ficolin-3 deficiency PMID: 18261799
  44. L-ficolin residue lysine57 is a key component of the interaction with both the mannan-binding lectin (MBL)-associated serine proteases and calreticulin, providing a strong indication that MBL and ficolins share homologous binding sites for their partners. PMID: 19109177
  45. Results suggest that the elevation of S-ficolin-3 and its association with specific manifestations in systemic lupus erythematosus may indicate a pathogenetic role of ficolin-3 in SLE. PMID: 19208603

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Involvement in disease Ficolin 3 deficiency (FCN3D)
Subcellular Location Secreted
Protein Families Ficolin lectin family
Tissue Specificity Liver and lung. In liver it is produced by bile duct epithelial cells and hepatocytes. In lung it is produced by both ciliated bronchial epithelial cells and type II alveolar epithelial cells.
Database Links

HGNC: 3625

OMIM: 604973

KEGG: hsa:8547

STRING: 9606.ENSP00000270879

UniGene: Hs.333383

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