Recombinant Human Forkhead box protein P3(FOXP3),partial

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Code CSB-EP861191HU1
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Description

The production of this Recombinant Human FOXP3 protein required the insertion of a DNA fragment (FOXP3, 1-260aa) into an expression vector and the transferral of this vector into E.coli cells (the step of transformation). The cells were then cultured and induced to express the FOXP3 protein. This recombinant protein was fused with N-terminal 6xHis tag. Its purity is 90%+ determined by SDS-PAGE.

FOXP3 (also called IPEX) is a gene encoding a protein called forkhead box protein P3 (FOXP3) (also known as scurfin) in human. The protein encoded by this gene can be cleaved into two chains, including forkhead box protein P3, C-terminally processed and forkhead box protein P3 41 kDa form. The FOXP3 protein can bind to specific regions of DNA and function as a master regulator of the regulatory pathway in regulating the immune system response. The FOXP3 protein is found primarily in the thymus (an immune system gland), where these regulatory T cells are produced.

Purity Greater than 90% as determined by SDS-PAGE.
Target Names FOXP3
Uniprot No. Q9BZS1
Research Area Transcription
Alternative Names AIID; DIETER; Forkhead box P3; Forkhead box protein P3; FOXP3; FOXP3_HUMAN; FOXP3delta7; Immune dysregulation polyendocrinopathy enteropathy X linked; Immunodeficiency polyendocrinopathy enteropathy X linked; IPEX; JM2; MGC141961; MGC141963; OTTHUMP00000025832; OTTHUMP00000025833; OTTHUMP00000226737; PIDX; Scurfin; XPID
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-260aa
Target Protein Sequence MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 31.7kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2. Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7. Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1. Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development. Inhibits the transcriptional activator activity of RORA. Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.
Gene References into Functions
  1. Results are in line with the hypothesis that in the early phase of ALS, neuroprotective helper T cells infiltrate in the affected areas in the lumbar spinal cord. This was reflected in higher peripheral percentage of CD4(+) helper T cells and higher expression of FOXP3 and IL-2Ralpha. PMID: 29574662
  2. RUNX3, a CD8(+) lineage-specific transcription factor, binds at the FOXP3-promoter to induce its transcription. PMID: 28487507
  3. Multivariate analysis of OS only found CD8(+)/Foxp3(+) ratio to be independent prognostic factor (P = 0.022) om spinal chordoma. PMID: 29051990
  4. Treg cells from asthmatic patients expressed more FOXP3 as well as GATA3; the expression level of GATA3 negatively correlated with FEV1%pred. Increased expressions of USP21 and PIM2 in Treg cells from asthmatic patients were found. PMID: 30013989
  5. Authors have demonstrated that excessive amounts of STAT5 may bind more TET2 to the FOXP3-TSDR and upregulate FOXP3 expression via DNA demethylation. Study improved the mechanism of FOXP3-TSDR hypomethylation in tumor-infiltrating CD4(+) T cells of CRC patients. PMID: 30013992
  6. Early-pregnancy decidual mRNA expression of the regulatory T-cell marker, FOXP3, was sixfold lower (p < 0.01) in pregnancies with a male fetus compared to pregnancies with a female fetus. PMID: 30003112
  7. In conclusion we suggest that genetic variants in FOXP3 gene may contribute to the pathogenesis of preeclampsia. PMID: 29206055
  8. Data show that the forkhead Box Protein P3 (FOXP3) response element at the -310 bp region, but not the -2182 bp region, is mainly required for ubiquitin conjugating enzyme 9 (UBC9) activation by FOXP3. PMID: 30011797
  9. The concurrent overexpression of FoxM1 and FoxP3 was evident in gastric cancer and inversely correlated with patient survival. PMID: 29804142
  10. Results suggest that -924A/G and +459T/C polymorphisms of the FOXP3 gene might be associated with unexplained recurrent spontaneous abortions (URSA) and -20G/A polymorphism is likely to be rare in Indian population and might not be associated with URSA. PMID: 29932060
  11. mutations can cause early-onset insulin-requiring diabetes with or without other features of IPEX syndrome PMID: 29193502
  12. The rs3761549, rs5902434, and rs2232365 are associated with an increased risk of acute graft-versus-host disease and decreased risk of post allogenic hematopoietic stem cell transplantation. PMID: 30027704
  13. Genetic variations at SNP rs3761548 in the FOXP3 gene represent a risk factor for peptic ulcer. PMID: 29938865
  14. Aberrant expression and methylation patterns of FoxP3 were detected in human T cells. PMID: 29901207
  15. blood mononuclear cells in stable CAD patients express a ratio of FOXP3 isoforms that is characteristic for activated CD4+ T cells PMID: 29100058
  16. this study suggested that macrophages in keloid tissues presented high activation status and were polarized toward the M2 subtype; moreover, these macrophages could promote Treg differentiation by upregulating Foxp3 expression. PMID: 29253537
  17. FOXP3 polymorphisms may be important markers to determine susceptibility to IIP or CTP-ILD in Chinese population. PMID: 28508461
  18. IPEX syndrome is associated with mutations in the forkhead box P3 (FOXP3) gene which causes impaired immune suppressive function. PMID: 28960390
  19. NOTCH1 is a central mediator of TGFbetamediated FOXP3 expression and NOTCH1 inhibition produces a significant reduction of melanoma cell proliferation and viability. PMID: 29620159
  20. The FOXP3 gene is one of the most important genes in the regulation of the immune cells. PMID: 29526847
  21. susceptibility to RSA was subject to the synthetic regulation of chromosomal aberrations and genetic mutations within CLTA-4 and Foxp3, suggesting that the conduction of karyotype analysis and genetic detection for RSA patients could effectively guide effective RSA counseling and sound child rearing. PMID: 29476189
  22. The ratio of cytotoxic (CD8(+) ) to regulatory (FoxP3(+) ) T-cells, in the intra-tumoral compartment, but not in the peri-tumoral compartment, can predict survival after resection of CRCLM. PMID: 29878369
  23. Combined results from many studies suggest that rs3761548 polymorphism is associated with cancer risk. [review] PMID: 30142808
  24. It was concluded that the abnormal expression of endometrial E2A existed in mid-secretory endometrium of women with recurrent miscarriage, and there was a positive correlation between E2A and FOXP3, and E2A and CTLA-4, suggesting the possible regulatory role of E2A in endometrium receptivity. PMID: 29270752
  25. this study provides evidence of the association of rs2294020 with systemic sclerosis evolution in female patients, modulating the time of progression from the diagnosis of early systemic sclerosis to the diagnosis of definite systemic sclerosis while no effect on systemic sclerosis susceptibility per se was found PMID: 29030005
  26. Lymphocytic enteritis associated to celiac disease shows an increase of FOXP3 expression and lymphocytes T-gammadelta that is not detected in other etiologies of enteritis. PMID: 28281276
  27. Downregulated expression of FOXP3 by siRNA transfection significantly inhibited cell proliferation and enhanced chemosensitivity to cisplatin in A549 lung adenocarcinoma cells. PMID: 28935177
  28. SNPs have a highly significant association with the risk of Graves' disease development in a Kashmiri population. PMID: 29890310
  29. Foxp3 is associated with lymphangiogenesis of cervical cancer. PMID: 28923073
  30. expressions in primary lesions as well as lymphogenic metastases appear to predict high-risk head and neck squamous cell carcinoma patients PMID: 28741409
  31. Polymorphic marker -3279 C>A of the FOXP3 gene was not associated with the risk of Pulmonary Sarcoidosis patients of Russian ethnicity. PMID: 29411762
  32. Data show that forkhead box P3 protein (FOXP3) protein not only binds with DNA sequences containing one FKH consensus sequence, but also binds with DNA sequences with two direct repeats of consensus sequences separated by three-nucleotides (DRE3). PMID: 28910978
  33. Study identifies CCR8+ regulatory T cells (Treg cells) as drivers of immunosuppression and provides compelling evidence of a self-feeding mechanism by which, at an autoimmune site, CCL1 produced by FOXp3+ Treg cells upregulates the expression of its own receptor, CCR8, on these cells, and potentiates their in vivo proliferation and suppressive activities as driver Treg cells. PMID: 28533380
  34. our findings suggest that FOXP3 suppresses tumor progression in hepatocellular carcinoma (HCC) via TGF-b/Smad2/3 signaling pathway, highlighting the role of FOXP3 as a prognostic factor and novel target for an optimal therapy against this fatal malignancy PMID: 28903735
  35. Studied lymphocyte phenotype in resected lymph nodes of patients with lung cancer by analyzing levels of Foxp3 and CD8 by immunohistochemical staining. PMID: 28831395
  36. We conclude that Foxp3 promoter polymorphisms are associated with susceptibility to endometrial cancer in Chinese Han women. PMID: 29718856
  37. Studied the association between Foxp3 gene polymorphisms and the susceptibility to differentiated thyroid cancers in a Chinese Han population. PMID: 27892628
  38. DLBCL cases with high FOXP3 have longer survival (P=0.03). T cells in the background of DLBCL may play a role in modulation of tumor progression. Their presence is associated with favorable prognostic parameters in DLBCL PMID: 26862953
  39. no significant association between rs3761548, rs2232365 polymorphisms of the FOXP3 gene, and an increased susceptibility to allergic rhinitis [meta-analysis] PMID: 28741671
  40. Zinc is capable of ameliorating the allogeneic immune reaction by enhancement of antigen-specific iTreg cells due to modulation of essential molecular targets by upregulation of Foxp3 and KLF-10 and downregulation of IRF-1. PMID: 27260002
  41. The Foxp3+ regulatory T cells (Tregs) play an indispensable role in controlling tolerance and immunity against self- and foreign antigens. PMID: 28696819
  42. study highlights the role of tissue differentiation on pathological response to neoadjuvant chemotherapy in gastric cancer and shows no impact between FOXP3, HER2 and MET expression in terms of tumor regression grading PMID: 29696715
  43. Flicr, a long noncoding RNA, modulates Foxp3 expression and autoimmunity. PMID: 28396406
  44. FOXP3 genotype association with breast cancer susceptibility and outcome depends on the breast cancer subtype. PMID: 28713192
  45. Results showed that physical activity in urban children was associated with lower FOXP3 promoter methylation, under conditions of high black carbon (BC) exposure. Reduced FOXP3 promoter methylation was associated with higher lung function. PMID: 28630656
  46. Findings indicate a transcriptional axis of FOXP3-BRCA1-miR-155 in breast cancer cells and show that plasma miR-155 may serve as a non-invasive biomarker for detection of early stage breast cancer. PMID: 28562349
  47. FOXP3 can act as a co-activator to facilitate the Wnt-b-catenin signaling pathway, inducing epithelial-mesenchymal transition and tumor growth and metastasis in non-small cell lung cancer. PMID: 28716029
  48. no significant association of FoxP3 promoter rs3761548 or (GT) n repeat length with presumed immunological graft failure. The genotype frequencies of Vav1 intron polymorphisms did not significantly differ between patients with graft failure and matched controls. PMID: 28470865
  49. Patients with the rs3761548 CC genotype showed better graft survival than those with the AC or AA genotype. Patients with the rs3761548 CC genotype also showed a lower rate of recurrence of the original glomerular disease than those with the AC or AA genotype. The frequency of acute rejection (AR) in patients with the rs2280883 TT genotype was lower than that in patients with the rs2280883 CT or CC genotype. PMID: 28643491
  50. we here revealed possible involvement of FOXP3 in regulating cCSC self-renewal via tuning COX2 expression, and thus providing a new target for the eradication of colon cancer stem cells. PMID: 28591725

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Involvement in disease Immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX)
Subcellular Location Nucleus. Cytoplasm.
Database Links

HGNC: 6106

OMIM: 300292

KEGG: hsa:50943

STRING: 9606.ENSP00000365380

UniGene: Hs.247700

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