Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Neuroscience
Alternative Names
38 kDa BFA-dependent ADP-ribosylation substrate; aging associated gene 9 protein; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; Epididymis secretory sperm binding protein Li 162eP; G3P_HUMAN; G3PD; G3PDH; GAPD; GAPDH; Glyceraldehyde 3 phosphate dehydrogenase; glyceraldehyde 3-PDH; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS; p38 component; OCT1 coactivator in S phase; 38-KD component; peptidyl cysteine S nitrosylase GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; wu:fb33a10
Species
Homo sapiens (Human)
Expression Region
2-335aa
Target Protein Sequence
GKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is expressed in E. coli using recombinant DNA technology. The expression region of the recombinant human includes the entire sequence of the mature human GAPDH protein (2-335aa), ensuring that the recombinant version retains all the functional domains and essential amino acid residues necessary for its biological activity. It carries an N-terminal 6xHis-SUMO tag, facilitating purification and enhancing protein expression and stability. Its purity is greater than 90% determined by SDS-PAGE. This recombinant human GAPDH protein can be utilized in neuroscience-related studies to investigate its enzymatic activity, protein-protein interactions, and cellular functions.
