Code | CSB-RA009232MA1HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:1000-1:5000 |
IHC | 1:20-1:200 |
IF | 1:20-1:200 |
FC | 1:20-1:200 |
The production of the GAPDH recombinant monoclonal antibody involves acquiring the GAPDH antibody genes, introducing these genes into suitable host cells, and employing a cellular expression and translation system to manufacture GAPDH antibodies. This approach offers several benefits, including a substantial enhancement in the purity and stability of the synthesized GAPDH recombinant monoclonal antibodies, along with improvements in antibody affinity and specificity. After synthesis, the GAPDH recombinant monoclonal antibody undergoes purification through affinity chromatography and undergoes extensive testing, including ELISA, WB, IHC, IF, and FC assays. Importantly, this antibody specifically targets the human GAPDH protein.
GAPDH's primary role is in glycolysis, where it participates in energy production and NADH generation. It also has diverse functions in cellular regulation, redox signaling, apoptosis, and RNA metabolism.
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