Code | CSB-MA000071M2m |
Size | US$200 |
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Full Product Name | Mouse anti-Human GAPDH Monoclonal antibody | ||||||||||
Uniprot No. | P04406 | ||||||||||
Target Names | GAPDH | ||||||||||
Alternative Names |
GAPDH; G3PD; GAPD; MGC88685
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Raised in | Mouse | ||||||||||
Species Reactivity | Human, Rat, Rabbit, Mouse | ||||||||||
Immunogen | Recombinant Human GAPDH protein (2-335AA) | ||||||||||
Immunogen Species | Human | ||||||||||
Conjugate | Non-conjugated | ||||||||||
Clonality | Monoclonal | ||||||||||
Isotype | IgG1 | ||||||||||
Clone No. | 10H4D6 | ||||||||||
Purification Method | >95%, Protein G purified | ||||||||||
Concentration | It differs from different batches. Please contact us to confirm it. | ||||||||||
Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
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Form | Liquid | ||||||||||
Tested Applications | ELISA, WB, IHC, IP, IF | ||||||||||
Recommended Dilution |
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Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunoprecipitation (IP) Protocol Immunofluorescence (IF) Protocol |
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Troubleshooting and FAQs | Antibody FAQs | ||||||||||
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||||||
Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
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Function |
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
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Gene References into Functions |
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Subcellular Location | Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. |
Protein Families | Glyceraldehyde-3-phosphate dehydrogenase family |
Database Links |
HGNC: 4141 OMIM: 138400 KEGG: hsa:2597 STRING: 9606.ENSP00000229239 UniGene: Hs.544577 |