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This GAPDH monoclonal antibody was raised by fusion of B lymphocytes with immortal cell cultures to produce hybridomas (A Recombinant Human GAPDH protein was used in the immunization process). Hybridomas would produce many copies of GAPDH monoclonal antibody. The specificity of this GAPDH monoclonal antibody makes it extremely efficient for binding of antigen within a mixture of GAPDH. In addition, this antibody has been validated in ELISA, WB, IHC, IP, IF.GAPDH (G3PD) is the abbreviation of glyceraldehyde-3-phosphate dehydrogenase, which is an enzyme in glycolysis and consists of 4 subunits of 30-40 kDa. The molecular weight is 146 kDa. The enzyme gene is a house-keeping gene, which is expressed at a high level in almost all tissues. The protein expression level in the same cell or tissue is generally constant and is not induced by the partial recognition sites contained. The influence of the substance remains constant, so it is widely used as a standardized internal reference for the extraction of total RNA, poly(A)+ RNA, Western blot and other experimental operations.
GAPDH is generally believed to mainly exert its glycolytic activity in the cytoplasmic matrix, but the latest research has shown that it also has a location on cells and biofilms. For example, when cells are stimulated, the content of GAPDH in the nucleus is significantly increased. When stimulated by neurotoxins, GAPDH first accumulates in the Golgi apparatus, and then enters the nucleus from the Golgi apparatus. The content of GAPDH in the nucleus of Xinhecheng GAPDH also increased significantly during oxidative stress. In addition, GAPDH is often seen on the biomembrane during membrane fusion and vesicle transport.
|Full Product Name||Mouse anti-Human GAPDH Monoclonal Antibody antibody|
GAPDH; G3PD; GAPD; MGC88685
|Species Reactivity||Human, Mouse, Rabbit|
|Immunogen||Recombinant Human GAPDH protein (2-335AA)|
|Purification Method||>95%, Protein G purified|
|Concentration||It differs from different batches. Please contact us to confirm it.|
|Buffer||Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
|Tested Applications||ELISA, WB, IHC, IP, IF|
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunofluorescence (IF) Protocol
|Troubleshooting and FAQs||Antibody FAQs|
|Storage||Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.|
|Lead Time||Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.|
Applications : Western Blot (WB)
Sample type: hek293 cell
Sample dilution: 1:5000
Review: 使用购买的二抗稀释液1:5000稀释此产品，4℃孵育过夜，效果较好，甚至比以前用过的1：1000的GAPDH抗体条带更加整齐明亮 The product was diluted 1:5000 with the purchased secondary antibody dilution and incubated overnight at 4°C. The results were better and even brighter than the previously used 1:1000 GAPDH antibody bands.
Applications : Immunoblot analyses
Sample type: cells
Review: Immunoblot analyses of protein levels of Hadha, Lclat1, and Lpcat3 across cardiac development. Images presented were representative of 2 experiments. Relative levels of individual proteins were normalized to that of GAPDH, and illustrated as bar plots on the lower panel.
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
|Gene References into Functions||
|Subcellular Location||Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton.|
|Protein Families||Glyceraldehyde-3-phosphate dehydrogenase family|