GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) primarily participates in the sixth step of the glycolytic pathway, where it catalyzes the oxidation of sugar molecules (such as glucose) within the cytoplasm to form 1,3-bisphosphoglycerate. This reaction simultaneously generates NADH (reduced form of nicotinamide adenine dinucleotide) and ATP (adenosine triphosphate). These energy and metabolic products are utilized in various cellular processes such as cell growth, metabolism, and movement. In addition to its role in glycolysis, GAPDH is involved in several non-metabolic processes. It participates in RNA transport and ribosome biogenesis within the cytoplasm and is also involved in processes like DNA repair and replication within the cell nucleus. These additional functions make GAPDH a multifunctional protein. Furthermore, GAPDH is implicated in certain pathological processes in specific diseases, underscoring the significance of studying its functions and regulatory mechanisms for a deeper understanding of cellular metabolism and disease mechanisms.
● GAPDH Recombinant Monoclonal Antibody CSB-RA009232MA1HU
Validated Data
Positive WB detected in: HepG2 whole cell lysate,U87 whole cell lysate,JK whole cell lysate
All lanes: GAPDH antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
IHC image of CSB-RA009232MA1HU diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cell with CSB-RA009232MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with CSB-RA009232MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
● GAPDH Monoclonal Antibody CSB-MA000071M1m
Validated Data
Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate,
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 10s
IHC image of CSB-MA000071M1m diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB
Immunofluorescence staining of Hela cells with(CSB-MA000071M1m)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L)
The following GAPDH reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
GAPDH Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA009232GA01HU | GAPDH Antibody |
Human,Mouse,Rat,Pig | ELISA,WB,IHC,IF |
CSB-PA00025B0Rb | GAPDH Antibody, HRP conjugated |
Human | ELISA |
CSB-PA00025A0Rb | GAPDH Antibody |
Human | ELISA, WB, IHC, IF |
CSB-PA00025D0Rb | GAPDH Antibody, Biotin conjugated |
Human | ELISA |
CSB-MA000071M0m | GAPDH Monoclonal Antibody |
Human, Rat, Rabbit | ELISA, WB, IHC, IP, IF, FC |
CSB-PA115482 | GAPDH Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-MA000195 | GAPDH Monoclonal Antibody |
H,R,M,Mk,Dg,C,Hm,Rb,Pg,Sh | ELISA,WB |
CSB-PA000351 | GAPDH Antibody |
Human,Mouse,Rat,Rabbit,Chicken,Monkey,Sheep,X | WB,IHC |
CSB-PA000364 | GAPDH Antibody |
Zebrafish | WB |
CSB-PA004666 | GAPDH Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA080240 | GAPDH Antibody |
Human,Mouse,Rat | WB, IHC |
CSB-PA779821 | GAPDH Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA00029A0Rb | GAPDH Antibody |
Human, Mouse | ELISA, WB, IHC, IF |
CSB-PA00029B0Rb | GAPDH Antibody, HRP conjugated |
Human | ELISA |
CSB-PA00029C0Rb | GAPDH Antibody, FITC conjugated |
Human | |
CSB-PA00029D0Rb | GAPDH Antibody, Biotin conjugated |
Human | ELISA |
CSB-RA009232A0HU | GAPDH Recombinant Monoclonal Antibody |
Human, Mouse | ELISA, WB |
CSB-RA009232MA1HU | GAPDH Recombinant Monoclonal Antibody |
Human | ELISA, WB, IHC, IF, FC |
GAPDH Antibodies for Chicken
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA009232HC01CH | GAPDH Antibody, FITC conjugated |
Chicken | |
CSB-PA009232HA01CH | GAPDH Antibody |
Chicken | ELISA |
CSB-PA009232HD01CH | GAPDH Antibody, Biotin conjugated |
Chicken | ELISA |
GAPDH Antibodies for H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh, Insect, Yeast
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-MA000184 | GAPDH Monoclonal Antibody |
H,R,M,Mk,Dg,C,Hm,Rb,Pg,Sh,Insect,Yeast | ELISA,WB,IHC |
GAPDH Antibodies for Cricetulus griseus
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA009232HA01DXU | GAPDH Antibody |
Cricetulus griseus | ELISA, WB |
GAPDH Antibodies for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA009232HC01DXU | GAPDH Antibody, FITC conjugated |
Cricetulus griseus | |
CSB-PA009232HD01DXU | GAPDH Antibody, Biotin conjugated |
Cricetulus griseus | ELISA |
CSB-PA009232HB01DXU | GAPDH Antibody, HRP conjugated |
Cricetulus griseus | ELISA |
GAPDH Antibodies for Danio rerio (Zebrafish) (Brachydanio rerio)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA009232XA01DIL | gapdh Antibody |
Danio rerio (Zebrafish) (Brachydanio rerio) | ELISA, WB (ensure identification of antigen) |
GAPDH Antibodies for Human
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-MA000071M1m | GAPDH Monoclonal Antibody |
Human, Mouse, Rabbit | ELISA, WB, IHC, IP, IF |
CSB-MA000071M2m | GAPDH Monoclonal Antibody |
Human, Rat, Rabbit, Mouse | ELISA, WB, IHC, IP, IF |
GAPDH Proteins for Cavia porcellus (Guinea pig)
Code | Product Name | Source |
---|---|---|
CSB-YP009232GU CSB-EP009232GU CSB-BP009232GU CSB-MP009232GU CSB-EP009232GU-B |
Recombinant Guinea pig Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP009232BO CSB-EP009232BO CSB-BP009232BO CSB-MP009232BO CSB-EP009232BO-B |
Recombinant Bovine Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP009232MO CSB-EP009232MO CSB-BP009232MO CSB-MP009232MO CSB-EP009232MO-B |
Recombinant Mouse Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Oryctolagus cuniculus (Rabbit)
Code | Product Name | Source |
---|---|---|
CSB-YP009232RB CSB-EP009232RB CSB-BP009232RB CSB-MP009232RB CSB-EP009232RB-B |
Recombinant Rabbit Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP009232CH CSB-BP009232CH CSB-MP009232CH |
Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast Baculovirus Mammalian cell |
CSB-EP009232CH | Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
E.coli |
CSB-EP009232CH-B | Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial,Biotinylated |
E.coli |
GAPDH Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP009232PI CSB-EP009232PI CSB-BP009232PI CSB-MP009232PI CSB-EP009232PI-B |
Recombinant Pig Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP009232HU CSB-BP009232HU CSB-MP009232HU CSB-EP009232HU-B |
Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP009232HU | Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
E.coli |
CSB-RP000254h | Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial |
E.coli |
GAPDH Proteins for Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Code | Product Name | Source |
---|---|---|
CSB-YP009232OEI CSB-EP009232OEI CSB-BP009232OEI CSB-MP009232OEI CSB-EP009232OEI-B |
Recombinant Oncorhynchus mykiss Glyceraldehyde-3-phosphate dehydrogenase (gapdh) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Columba livia (Rock dove)
Code | Product Name | Source |
---|---|---|
CSB-YP009232DVK CSB-EP009232DVK CSB-BP009232DVK CSB-MP009232DVK CSB-EP009232DVK-B |
Recombinant Columba livia Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Meleagris gallopavo (Wild turkey)
Code | Product Name | Source |
---|---|---|
CSB-YP009232MQV CSB-EP009232MQV CSB-BP009232MQV CSB-MP009232MQV CSB-EP009232MQV-B |
Recombinant Meleagris gallopavo Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Mustela putorius furo (European domestic ferret) (Mustela furo)
Code | Product Name | Source |
---|---|---|
CSB-YP009232MOH CSB-EP009232MOH CSB-BP009232MOH CSB-MP009232MOH CSB-EP009232MOH-B |
Recombinant Mustela putorius furo Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Code | Product Name | Source |
---|---|---|
CSB-YP009232DXU CSB-BP009232DXU CSB-MP009232DXU CSB-EP009232DXU-B |
Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP009232DXU | Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial |
E.coli |
GAPDH Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP009232XBE CSB-EP009232XBE CSB-BP009232XBE CSB-MP009232XBE CSB-EP009232XBE-B |
Recombinant Xenopus laevis Glyceraldehyde-3-phosphate dehydrogenase (gapdh) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Mesocricetus auratus (Golden hamster)
Code | Product Name | Source |
---|---|---|
CSB-YP009232MRG CSB-EP009232MRG CSB-BP009232MRG CSB-MP009232MRG CSB-EP009232MRG-B |
Recombinant Mesocricetus auratus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP009232RA CSB-EP009232RA CSB-BP009232RA CSB-MP009232RA CSB-EP009232RA-B |
Recombinant Rat Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis)
Code | Product Name | Source |
---|---|---|
CSB-YP009232PEL CSB-EP009232PEL CSB-BP009232PEL CSB-MP009232PEL CSB-EP009232PEL-B |
Recombinant Pelodiscus sinensis Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Code | Product Name | Source |
---|---|---|
CSB-YP009232PYX CSB-EP009232PYX CSB-BP009232PYX CSB-MP009232PYX CSB-EP009232PYX-B |
Recombinant Pongo abelii Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)
Code | Product Name | Source |
---|---|---|
CSB-YP009232DIL CSB-EP009232DIL CSB-BP009232DIL CSB-MP009232DIL CSB-EP009232DIL-B |
Recombinant Danio rerio Glyceraldehyde-3-phosphate dehydrogenase (gapdh) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Felis catus (Cat) (Felis silvestris catus)
Code | Product Name | Source |
---|---|---|
CSB-YP009232CA CSB-EP009232CA CSB-BP009232CA CSB-MP009232CA CSB-EP009232CA-B |
Recombinant Cat Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Ovis aries (Sheep)
Code | Product Name | Source |
---|---|---|
CSB-YP009232SH CSB-EP009232SH CSB-BP009232SH CSB-MP009232SH CSB-EP009232SH-B |
Recombinant Sheep Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
Code | Product Name | Source |
---|---|---|
CSB-YP009232DO CSB-EP009232DO CSB-BP009232DO CSB-MP009232DO CSB-EP009232DO-B |
Recombinant Dog Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Glossina morsitans morsitans (Savannah tsetse fly)
Code | Product Name | Source |
---|---|---|
CSB-YP009232GGM CSB-EP009232GGM CSB-BP009232GGM CSB-MP009232GGM CSB-EP009232GGM-B |
Recombinant Glossina morsitans morsitans Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E13911h | Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH/G3PDH) ELISA kit |
serum, plasma, tissue homogenates, cell lysates | 7.81 pg/mL |