Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

GPD1

Uniprot No.

P21695

Research Area

Signal Transduction

Alternative Names

AI747587; cytoplasmic; EC 1.1.1.8; FLJ26652; G3PD; Gdc-1; Gdc1; Gdp1; Glycerol 3 phosphate dehydrogenase 1; Glycerol 3 phosphate dehydrogenase cytosolic; Glycerol 3 phosphate dehydrogenase soluble; Glycerol-3-phosphate dehydrogenase [NAD+]; Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; Glycerol-3-phosphate dehydrogenase 1 (soluble); Glycerol-3-phosphate dehydrogenase; Glycerol-3-phosphate dehydrogenase, soluble ; Glycerphosphate dehydrogenase; GPD-C; gpd1; Gpd1 protein; Gpd3; GPDA_HUMAN; Gpdc; GPDH; GPDH-C; Gpdhc; HTGTI; KIAA4010; MGC93453; MGPD; mKIAA4010

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-349aa

Target Protein Sequence

MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

53.6kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Human GPD1 recombinant protein was produced in E.coli, where the gene sequence encoding Human GPD1 (1-349aa) was expressed with the N-terminal 6xHis-SUMO tag. The purity of this GPD1 protein was greater than 90% by SDS-PAGE.
GPD1 is an enzyme that catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate inside cells. This reaction is a critical step in the glycerolipid metabolism pathway. The enzyme plays a significant role in energy metabolism and fatty acid synthesis. The GPD1 protein is involved in regulating the synthesis of triglycerides, which is one way the body stores energy. It is also associated with glucose metabolism and insulin response, closely linked to the regulation of blood sugar levels. GPD1 protein is widely expressed in various tissues, including the liver, muscles, and adipose tissue. This reflects its different biological functions in different tissues. Some studies suggest that GPD1 is associated with metabolic diseases such as obesity and diabetes. Abnormal activity or expression levels of GPD1 may be related to the onset and development of these diseases.

Target Background

Function

Has glycerol-3-phosphate dehydrogenase activity.

Gene References into Functions

To recognize this entity by considering GPD1 mutations in appropriate clinical settings. PMID: 27368975
Although these data indicate that a lack of GPD1 inhibits gluconeogenesis from glycerol, chronic GPD1 deficiency may induce an adaptation that enhances gluconeogenesis from glycogenic amino acids PMID: 27733253
The R269A mutation of GPDH1 results in a 110-fold increase in K(m) (2.8 kcal/mol effect) and a 41,000-fold decrease in k(cat) (6.3 kcal/mol effect), which corresponds to a 9.1 kcal/mol destabilization of the transition state. PMID: 25884759
massive hepatomegaly, fatty liver and severe hypertriglyceridemia carrying a compound heterozygous mutation in GPD1 PMID: 24549054
Mutation analysis revealed a homozygous splicing mutation, c.361-1G>C, which resulted in an aberrantly spliced mRNA in the ten affected individuals. PMID: 22226083
The expression levels of the GPD1 gene did not differ between patients with OSA and their matched controls. The results were not affected by the clinical and biochemical measurements, the sleep parameters or the severity of nocturnal hypoxemia. PMID: 20404019
Identification and function of mutations in FAD-binding domain of mitochonrial glycerophosphate dehydrogenase in caucasians with type 2 diabetes mellitus. PMID: 11822825
GPD1 has a role in increased triacylglycerol production in adipose tissue of obese humans PMID: 14674682
The apoenzyme structure of GPD1 has been determined; an electrophilic catalytic mechanism by the epsilon-NH3+ group of Lys204 is proposed on the basis of structural analyses. PMID: 16460752
GpD1 is a weight-loss-responsive gene in skeletal muscle. Its observed transcriptional modulation may decrease triglyceride synthesis, with weight loss. PMID: 16849634
The activities of glycerol phosphate dehydrogenase were decreased by 73% in pancreatic islets of patients with type 2 diabetes. PMID: 19296078
the discovery overexpression of GPD1 and RRBP1 proteins and lack of expression for HNRNPH1 and SERPINB6 proteins which are new candidate biomarkers of colon cancer. PMID: 19425502

Hide All

Involvement in disease

Hypertriglyceridemia, transient infantile (HTGTI)

Subcellular Location

Cytoplasm.

Protein Families

NAD-dependent glycerol-3-phosphate dehydrogenase family

Tissue Specificity

Expressed in liver (at protein level).

Database Links

HGNC: 4455

OMIM: 138420

KEGG: hsa:2819

STRING: 9606.ENSP00000301149

UniGene: Hs.524418

Code	CSB-EP009709HU
Abbreviation	Recombinant Human GPD1 protein
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Glycerol-3-phosphate dehydrogenase [NAD (+)], Cytoplasmic domain (GPD1)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage