Recombinant Human Guanine nucleotide-binding protein G (z) subunit alpha (GNAZ)

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Code CSB-EP009601HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
GNAZ
Uniprot No.
Research Area
Signal Transduction
Alternative Names
G(x) alpha chain; GNAZ; GNAZ_HUMAN; Guanine nucleotide-binding protein G(z) subunit alpha; Gz-alpha
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
2-355aa
Target Protein Sequence
GCRQSSEEKEAARRSRRIDRHLRSESQRQRREIKLLLLGTSNSGKSTIVKQMKIIHSGGFNLEACKEYKPLIIYNAIDSLTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADPGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIFCVELSGYDLKLYEDNQTSRMAESLRLFDSICNNNWFINTSLILFLNKKDLLAEKIRRIPLTICFPEYKGQNTYEEAAVYIQRQFEDLNRNKETKEIYSHFTCATDTSNIQFVFDAVTDVIIQNNLKYIGLC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
56.8kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Amino acids 2-355 constitute the expression domain of recombinant Human GNAZ. The calculated molecular weight for this GNAZ protein is 56.8 kDa. The GNAZ protein was expressed in e.coli. The GNAZ gene fragment has been modified by fusing the N-terminal 6xHis-SUMO tag, providing convenience in detecting and purifying the recombinant GNAZ protein during the following stages.

The human guanine nucleotide-binding protein G(z) subunit alpha (GNAZ) is a member of the G protein subunit alpha z family. GNAZ is involved in transmitting signals from G protein-coupled receptors (GPCRs) to intracellular effector proteins. It plays a role in regulating adenylate cyclase activity, modulating the levels of intracellular cyclic AMP (cAMP). The dynamic regulation of cAMP is crucial for various cellular processes, including cell signaling, metabolism, and neurotransmission. GNAZ's involvement in GPCR signaling highlights its significance in mediating responses to extracellular stimuli and its potential impact on various physiological and pathological conditions.

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Target Background

Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Gene References into Functions
  1. GNAZ mutations are associated with melanoma. PMID: 20424519
  2. cGMP-phosphodiesterase 6 can be activated by another cancer-retina antigen, transducin, through Wnt5a-Frizzled-2 cascade, which leads to a lowering of cGMP and an increase in intracellular calcium mobilization. PMID: 19946729
  3. findings suggest that Galphaz signals may attenuate Rho-induced stimulation of SRF-mediated transcription PMID: 15326221
Subcellular Location
Membrane; Lipid-anchor.
Protein Families
G-alpha family, G(i/o/t/z) subfamily
Database Links

HGNC: 4395

OMIM: 139160

KEGG: hsa:2781

STRING: 9606.ENSP00000248996

UniGene: Hs.584760

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