Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

GNAZ

Uniprot No.

P19086

Research Area

Signal Transduction

Alternative Names

G(x) alpha chain; GNAZ; GNAZ_HUMAN; Guanine nucleotide-binding protein G(z) subunit alpha; Gz-alpha

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-355aa

Target Protein Sequence

GCRQSSEEKEAARRSRRIDRHLRSESQRQRREIKLLLLGTSNSGKSTIVKQMKIIHSGGFNLEACKEYKPLIIYNAIDSLTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADPGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIFCVELSGYDLKLYEDNQTSRMAESLRLFDSICNNNWFINTSLILFLNKKDLLAEKIRRIPLTICFPEYKGQNTYEEAAVYIQRQFEDLNRNKETKEIYSHFTCATDTSNIQFVFDAVTDVIIQNNLKYIGLC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

56.8kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 2-355 constitute the expression domain of recombinant Human GNAZ. The calculated molecular weight for this GNAZ protein is 56.8 kDa. The GNAZ protein was expressed in e.coli. The GNAZ gene fragment has been modified by fusing the N-terminal 6xHis-SUMO tag, providing convenience in detecting and purifying the recombinant GNAZ protein during the following stages.

The human guanine nucleotide-binding protein G(z) subunit alpha (GNAZ) is a member of the G protein subunit alpha z family. GNAZ is involved in transmitting signals from G protein-coupled receptors (GPCRs) to intracellular effector proteins. It plays a role in regulating adenylate cyclase activity, modulating the levels of intracellular cyclic AMP (cAMP). The dynamic regulation of cAMP is crucial for various cellular processes, including cell signaling, metabolism, and neurotransmission. GNAZ's involvement in GPCR signaling highlights its significance in mediating responses to extracellular stimuli and its potential impact on various physiological and pathological conditions.

Target Background

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Gene References into Functions

GNAZ mutations are associated with melanoma. PMID: 20424519
cGMP-phosphodiesterase 6 can be activated by another cancer-retina antigen, transducin, through Wnt5a-Frizzled-2 cascade, which leads to a lowering of cGMP and an increase in intracellular calcium mobilization. PMID: 19946729
findings suggest that Galphaz signals may attenuate Rho-induced stimulation of SRF-mediated transcription PMID: 15326221

Subcellular Location

Membrane; Lipid-anchor.

Protein Families

G-alpha family, G(i/o/t/z) subfamily

Database Links

HGNC: 4395

OMIM: 139160

KEGG: hsa:2781

STRING: 9606.ENSP00000248996

UniGene: Hs.584760

Code	CSB-EP009601HU
Abbreviation	Recombinant Human GNAZ protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Guanine nucleotide-binding protein G (z) subunit alpha (GNAZ)

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