Recombinant Human HLA class II histocompatibility antigen, DQ alpha 1 chain (HLA-DQA1), partial

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Code CSB-EP365686HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
HLA-DQA1
Uniprot No.
Research Area
Immunology
Alternative Names
CD; CELIAC1; DC 1 alpha chain; DC alpha; DC-1 alpha chain; DC-alpha; DC1; included; DQ alpha 1 chain; DQ-A1; DQ-DRW9 alpha chain; DQA1_HUMAN; FLJ27088; FLJ27328; Gluten-sensitive enteropathy (celiac disease); GSE; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen; DQ alpha 1 chain; HLA class II histocompatibility antigen; DQ(W3) alpha chain; HLA-DCA; HLA-DQA; HLA-DQA1; HLA-DQA1 major histocompatibility complex; class II; DQ alpha 1; HLADC histocompatibility type; Immune response antigens HIa; included; leucocyte antigen DQA1 ; leukocyte antigen alpha chain ; LOC100133678; LOC100507686; LOC100509457; Major histocompatibility complex; class II; DQ alpha 1; MGC149527; MHC class II antigen; MHC class II DQA1; MHC class II HLA-D alpha glycoprotein; MHC class II HLA-DQ alpha 1; MHC class II surface glycoprotein; MHC HLA-DQ alpha; OTTHUMP00000029141; OTTHUMP00000176885; OTTHUMP00000178551; OTTHUMP00000178552; OTTHUMP00000233817
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
24-213aa
Target Protein Sequence
EDIVADHVASYGVNLYQSYGPSGQYTHEFDGDEQFYVDLGRKETVWCLPVLRQFRFDPQFALTNIAVLKHNLNSLIKRSNSTAATNEVPEVTVFSKSPVTLGQPNILICLVDNIFPPVVNITWLSNGHSVTEGVSETSFLSKSDHSFFKISYLTLLPSAEESYDCKVEHWGLDKPLLKHWEPEIPAPMSE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
25.4kDa
Protein Length
Extracellular Domain
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Amino acids 24-213 form the expressed segment for recombinant Human HLA-DQA1. The calculated molecular weight for this HLA-DQA1 protein is 25.4 kDa. The HLA-DQA1 protein was expressed in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of HLA-DQA1, making it easier to detect and purify the HLA-DQA1 recombinant protein in the later stages of expression and purification.

The human HLA class II histocompatibility antigen, DQ alpha 1 chain (HLA-DQA1) is a subunit of the HLA class II complex, which is involved in presenting antigens to immune cells. HLA-DQA1 plays a crucial role in the immune system. HLA-DQA1 is expressed on the surface of antigen-presenting cells, such as dendritic cells, macrophages, and B cells. It interacts with the beta chain (HLA-DQB1) to form a heterodimer, creating a binding groove for the presentation of antigenic peptides to CD4+ T cells. This process is essential for the activation of the adaptive immune response. Research on HLA-DQA1 focuses on understanding its role in antigen presentation, immune response regulation, and its association with autoimmune diseases and susceptibility to infections.

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Target Background

Function
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Gene References into Functions
  1. The analysis showed a significant association between rs2187668-(A) allele and idiopathic membranous nephropathy susceptibility, and the intervention of this mutation might bring new therapeutic strategy for idiopathic membranous nephropathy PMID: 30383665
  2. we described differences in expression of DQA1 and DQB1 gene alleles in whole-blood cells, monocytes and B lymphocytes. Our findings support the idea that expression level of HLA class II alleles is the result of both promoter- and cell-specific factors. PMID: 29663655
  3. The C>G mutation in the HLA-DQA1 gene was associated with the occurrence of Spinal tuberculosis. PMID: 29795056
  4. Allelic frequency distributions for SNP rs2187668 within HLA-DQA1 were significantly different between the idiopathic membranous nephropathy and control groups. PMID: 28849274
  5. results revealed that a novel independent variant in the promoter of HLA-DQA1 was associated with idiopathic membranous nephropathy in Chinese Han population PMID: 28685717
  6. in the Iranian population, HLA-DQA1*0201 appears to have protective role PMID: 28919585
  7. Using genome-wide association meta-analysis authors discover two regions associated with longevity (HLA-DQA1/DRB1 and LPA). PMID: 29030599
  8. HLA-DQA1 was expressed more than 9-fold higher in high-load hepatitis C virus liver patients than in low-load HCV liver patients. PMID: 29248968
  9. HLA-DQ4 positive patients have a higher risk of Vogt-Koyanagi-Harada disease. In the results of alleles, risk factor is HLA-DQA1*0301, while protective ones may include HLA-DQA1*0103, 0401, 0501. PMID: 29443768
  10. APOBEC3A/B and HLA-DQA1 are powerful biomarkers for systemic sclerosis risk evaluation and contribute to the susceptibility to systemic sclerosis. PMID: 27036383
  11. The GG genotype of HLA-DQ rs9277346 was associated with hepatitis B virus infection in the Chinese Han population. PMID: 27291710
  12. Single-nucleotide polymorphism in HLA-DQA1 gene is associated with primary membranous nephropathy. PMID: 26673907
  13. The presence of the haplotype HLA-DPA1*03 DQA*05 was associated with allergic asthma, and the presence of HLA-DPA1*03 and the absence of HLA-DQA*05 with nonallergic asthma. PMID: 28380482
  14. This study found that, among Caucasian patients treated with BoNT/A, DQA1*01:02 are higher in Antibody-positive than in antibody-negative patients. PMID: 28385185
  15. Frequency of DQA1*05 was increased in patients with juvenile-onset systemic sclerosis versus controls. PMID: 27214100
  16. In the present study, a discriminatory potential of HLA-DQA1/B1 alleles to identify susceptibility to congenital toxoplasmosis and the most severe cases has been shown. PMID: 26856406
  17. The protective influence of DRB1*15:01-DQA1*01:02-DQB1*06:02 spans from autoantibody development through all stages of progression, and relatives with this allele only rarely develop T1 diabetes. PMID: 26822082
  18. this study shows detailed DNA methylation status and its correlation with expression of each HLA-DQA1 allele in patients with type 1 diabetes mellitus PMID: 26854762
  19. The population of a Portuguese Madeira Island is distinguished by the substantial HLA-DQA1 and HLA-DQB1 allele diversity. PMID: 28032448
  20. Patients with Latent Autoimmune Diabetes in Adults (LADA) have higher prevalence of thyroid autoimmune diseases. In patients with LADA similarly to type 1 genotype DQA1*0301 seems to CONFER susceptibility to thyroid autoimmunity, and DQB1*0201 to celiac disease. PMID: 26884287
  21. HLA-DQ rs9275319 showed a significant association with HBV infection and with natural clearance. PMID: 27236152
  22. the DQA1*01:02:01G allele frequency was significantly lower (Pc=0.042) among newborns born by women with severe preeclampsia/eclampsia compared with controls. PMID: 27121092
  23. Two missense SNPs, rs12722042 and 12722039, in the HLA-DQA1 gene yielded the highest effect sizes (odds ratio [OR] approximately 14; P <0.01) for sex-specific results. The HLA-DQA1 SNPs belong to DQA1*01 and confirmed the previously reported male-specific association with DQA1*01. This finding supports the proposed infection-related etiology in childhood ALL risk for males. PMID: 27861356
  24. A possible pathogenic link that HLA-DQ2/DQ8 positivity, in presence of exogenous still unknown stimuli, may favor an immune condition with detrimental effects during early stages of pregnancy. PMID: 26883458
  25. Genetic polymorphism of the HLADQA1 gene in Recurrent Respiratory Papillomatosis patients in combination with carriage of type 16 human papilloma virus can be used for the prognosis of the severity HLADQA1of this disease. PMID: 27500575
  26. The Sonora, Mexico HLA-DQ risk heterodimer proportion was 16.1% for HLA-DQ2 and 13.6% for HLA-DQ8, with an HLA-DQ2:HLA-DQ8 ratio of 1.2:1. The DQ8/DQ2 genotype represented a 1:14 risk for type 1 diabetes, whereas the DQ8/DQB1*0201 combination showed a 1:6 risk for celiac disease. PMID: 26088570
  27. HLA-DQA1*0102 and HLA-DQB1*0201 alleles may be involved in the production of anti-beta2-glycoprotein I antibody in recurrent miscarriage patients. PMID: 26818121
  28. DQA1*0501 had significant negative association with MuSK myasthenia gravis. PMID: 26671138
  29. We found association of three variants in the region harboring genes encoding the class II human leukocyte antigens (HLAs): both located between HLA-DQA1 and HLA-DRB1 PMID: 26829749
  30. Pharmacoresistant temporal lobe epilepsy associated with mesial hippocampal sclerosis is not determined by HLA class II alleles in the DRB1, DQB1, and DQA1 regions but the HLA DRB1*1302 allele exhibited a tendency to behave as a susceptibility factor PMID: 26362370
  31. Suggest that the DQA1*0103/CD74 dimer may result in presentation of unique antigens and susceptibility to develop arthritis. PMID: 26524976
  32. HLA-DQA1 alleles contribute to the susceptibility of unexplained recurrent spontaneous abortion among ethnic Han Chinese. PMID: 26829741
  33. No significant association with tuberculosis risk was found in any HLA-DQA1 allele. PMID: 26007157
  34. The protective effects against type 1 diabetes mellitus of HLA-DQA1*01:01, HLA-DQB1*05:03, *06:02, *06:03, and *06:04 were robustly suggested by all indicators of meta-analyses. (Meta-analysis) PMID: 26095634
  35. In Asian population, the protective gene HLA-DQB1*0303 and the susceptible genes HLA-DQB1*0401, HLA-DQA1*0103 and HLA-DQA1*0301 in Helicobacter pylori infection were established by meta-analysis. PMID: 25773770
  36. A three-way interaction between SNPs in HLA-DQ and GNLY was identified in terms of HBV clearance. PMID: 25644528
  37. lamotrigine-induced cutaneous adverse drug reactions are associated with HLA-DRB1*0405, -DQB1*0401 and -DQA1*0303 PMID: 25845749
  38. Our results showed significant association of 10 single nucleotide polymorphisms (SNPs) with T1D at p<0.01, including HLA-DQA1/rs9272346, whereas 5 more SNPs showed their association at 0.01 PMID: 25661663
  39. Data indicate interactions for 11 of 21 pairs of common HLA-DRB1-HLA-DQA1-HLA-DQB1 haplotypes. PMID: 26168013
  40. Closed-tube human leukocyte antigen DQA1 *05 genotyping assay based on switchable lanthanide luminescence probes. PMID: 25120130
  41. The HLA typing results revealed that HLA DQA1*0501, HLA DQB1*0302, and HLA DRB1*11 alleles were common in all four family members with focal epithelial hyperplasia. HLA DRB1*04 allele was detected in three of them. PMID: 24738569
  42. HLA-DQA1 missense mutation is a genetic risk factor in the development of steroid sensitive nephrotic syndrome. PMID: 25349203
  43. In this study, we found that the SNP of rs9272219 in HLA-DQA1 is a potential susceptibility locus in rheumatoid arthritis of Han Chinese population PMID: 25550865
  44. highlights a potential contribution to MS risk also from interisotypic combination between products of neighboring HLA-DR15 haplotype alleles, in this case the DQA1/DRB1 combination. PMID: 25911099
  45. The most frequent alleles were HLA-DQA1*03:01 (13.1%) in Kazakh population. PMID: 25531278
  46. A significant correlation was found between worldwide wheat consumption and HLA DQ2 (p=0.01) and the sum of DQ2 and DQ8 (p=0.01) frequencies. Wheat consumption and HLA-DQ2 tend to co-localize in different continents. PMID: 25200477
  47. The HLA-DQ2/2 genotype may predispose to obesity among 2-4-year-old children with genetic risk for type 1 diabetes. PMID: 24694666
  48. all CD patients had at least one of the CD-associated alleles, and the highest CD risk was indicated by the presence of the HLA-DQ2.5 heterodimer (HLA-DQA1*05-DQB1*02) with HLA-DQB1*02 in homozygosity. PMID: 25413104
  49. DQA1 carriage, allele and DQA1-DQB1 and DRB1-DQA1-DQB1 haplotype frequencies, linkage disequilibria and related values are presented. PMID: 25345618
  50. Data show there was strong epistasis between HLA-DQA1 single nucleotide polymorphism rs2187668 and the phospholipase A2 receptor 1 (PLA2R1) variant rs35771982. PMID: 25187357

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
Protein Families
MHC class II family
Database Links

HGNC: 4942

OMIM: 146880

KEGG: hsa:3117

UniGene: Hs.387679

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