Recombinant Human Histone H3.2 (HIST2H3A)

Code CSB-YP744479HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP744479HU
MSDS
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Source E.coli
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Code CSB-EP744479HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP744479HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP744479HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HIST2H3A;
Uniprot No.
Alternative Names
HIST2H3A;; HIST2H3C; H3F2; H3FM;; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o
Species
Homo sapiens (Human)
Expression Region
2-136
Target Protein Sequence
ARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEASEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene References into Functions
  1. Finally, mutations of H3G34 or H3P38 alleviate the inhibitory effects of H3K36M on H3K36 methylation, demonstrating that the stable interaction of H3K36M with SETD2 is critical for its inhibitory effects. PMID: 28256625
  2. Studies presented here confirm that the adenovirus 2 large E1A protein activation domain interacts with p300, as reported previously (P. Pelka, J. N. G. Ablack, J. Torchia, A. S. Turnell, R. J. A. Grand, J. S. Mymryk, Nucleic Acids Res 37:1095-1106, 2009), and that the resulting acetylation of H3K18/27 affects varied steps in transcription at different viral promoters. PMID: 29976669
  3. This study presented the H3 K27M mutations in posterior fossa ependymomas. PMID: 27539613
  4. Findings point to epigenetic mark H3K27me3 as an important event in prostate carcinogenesis and progression. PMID: 28403887
  5. Study identified phosphorylation of histone H3 at serine 10 (H3S10ph) as a histone post-translational modifications with most consistent and significant difference between gastric cancer (GC) tumor and negative resection margin. H3S10 phosphorylation in GC is mediated by MSK1 through the p38- MAPK pathway. PMID: 27588146
  6. Results showed that high levels of MMSET in the myeloma-like cells drove the formation of hypermethyled proteoforms containing H3K36me2 co-existent with the repressive marks H3K9me2/3 and H3K27me2/3. PMID: 26272979
  7. Results identified histone H3K27me3 as elevated in aggressive melanoma cells relative to less aggressive melanoma cells in cell culture and in patient tissues. PMID: 26621846
  8. Study shows proteolytic processing of H2B and H3 in hepatocytes where four different clipping sites were localized in H3 and one in H2B. Also, clipped H3 carry distinct co-existing post-translational modifications but different from those in intact H3. PMID: 26424599
  9. Locally generated fumarate inhibits KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 Lys 36. PMID: 26237645
  10. In vitro clipping assays confirmed the ability of both Neisseria meningitidis proteins, App and MspA, to proteolytically cleave the core histone H3. PMID: 25600171
  11. H3T80ph, protruding from the nucleosome surface, promotes interactions between adjacent nucleosomes to promote chromatin compaction during mitosis in metazoan cells. PMID: 24275038
  12. UHRF1 PHD finger (including the preceding zinc-Cys4 knuckle) acts together with the adjacent double Tudor domain to specifically recognize the H3K9me3 mark. PMID: 22100450
  13. NASP balances the activity of the heat shock proteins Hsc70 and Hsp90 to direct H3-H4 for degradation by chaperone-mediated autophagy PMID: 22195965
  14. purification of a novel JmjC domain-containing protein, JHDM1 (JmjC domain-containing histone demethylase 1), that specifically demethylates histone H3 at lysine 36 PMID: 16362057
  15. BirA ligase biotinylates K4, K9, K18 and K23 in histone H3. PMID: 18452652
  16. Sustained JNK1 activation is associated with altered histone H3 methylations in human liver cancer. PMID: 19041150
  17. phosphorylation of H3T45 (H3T45ph) increases dramatically in apoptotic cells, around the time of DNA nicking PMID: 19363025
  18. The acetylation of lysine(56) residue of Histone H3 is critical for genomic stability and DNA damage response. PMID: 19411844
  19. Acetylation of H3 K56 does not directly affect the compaction of chromatin and has modest effects on chromatin remodeling. However, H3 K56 acetylation increases DNA breathing 7-fold. PMID: 19818718

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Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H3 family
Database Links

HGNC: 20505

OMIM: 142780

KEGG: hsa:126961

STRING: 9606.ENSP00000385479

UniGene: Hs.647745

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