Recombinant Human Histone acetyltransferase KAT5(KAT5),partial

In Stock
Code CSB-RP178494h
Size US$657
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP178494h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) KAT5.

  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP178494h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) KAT5.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names KAT5
Uniprot No. Q92993
Research Area Immunology
Alternative Names 60 kDa Tat interactive protein; 60 kDa Tat-interactive protein; cPLA(2) interacting protein; cPLA(2)-interacting protein; cPLA2; cPLA2 interacting protein; ESA1; Histone acetyltransferase HTATIP; Histone acetyltransferase KAT5; HIV 1 Tat interactive protein; HIV 1 Tat interactive protein, 60kDa; HIV-1 Tat interactive protein; HTATIP; HTATIP1; K(lysine) acetyltransferase 5; K-acetyltransferase 5; KAT5; KAT5_HUMAN; Lysine acetyltransferase 5; PLIP; Tat interacting protein, 60kDa; TIP; Tip60
Species Homo sapiens (Human)
Source E.coli
Expression Region 3-513aa
Target Protein Sequence EVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLPIPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 62.4kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2. Promotes FOXP3 acetylation and positively regulates its transcriptional repressor activity
Gene References into Functions
  1. Results show that KAT5 expression is decreased in prostate cancer (PCa) and correlated with shorter recurrence-free survival. PMID: 30142696
  2. Tip60 suppressed growth and metastasis throughout the progression of cholangiocarcinoma and identified the PI3K/AKT pathway as a dominant signal of Tip60. PMID: 30308494
  3. TIP60 is involved in several adipogenesis mechanisms through its interaction with three important proteins: PPARc, USP7, and GPR50. These actors act also in different processes of breast cancer development. PMID: 28873018
  4. These findings demonstrate the critical regulation of TIP60/p53 pathway in apoptosis upon metabolic stress and provide a novel insight into the down-regulation of TIP60 in tumor cells. PMID: 29174981
  5. Our data demonstrate for the first time that TIP60 through its MYST domain directly interacts with UHRF1 PMID: 29268763
  6. These results suggest that TIP60, in concert with other cellular factors, plays an important role in the regulation of the HBV chromatin structure by acting as a critical component of the intrinsic antiviral defense, which sheds new light on the regulation of HBV replication. PMID: 29321313
  7. Irreversible inhibition of USP7 results in durable downstream biological responses in cells, including down-regulation of Tip60 and consequent impairment of Treg suppressive function PMID: 29236775
  8. most HIF1A targets require either TIP60, the CDK8-Mediator complex, or both as coactivators for full expression in hypoxia. PMID: 27320910
  9. NOTCH1 inhibits activation of ATM by impairing the formation of an ATM-FOXO3a-KAT5 complex. PMID: 27524627
  10. Collectively, the data establish a hitherto unknown liaison among MDR1, BMI1 and TIP60 and provide mechanistic insights into cisplatin-induced MDR1 expression resulting in acquired cross-resistance against paclitaxel, doxorubicin and likely other anticancer drugs. PMID: 27295567
  11. TIP60-mediated growth suppression of HPV-induced cervical cancer is mediated in part due to TERT repression through Sp1 acetylation. In summary, our study has identified a novel substrate for TIP60 catalytic activity and a unique repressive mechanism acting at the TERT promoter in virus-induced malignancies. PMID: 29045464
  12. These findings reveal that Endoplasmic reticulum stress engages the GSK3beta-TIP60-ULK1 pathway to increase autophagy. PMID: 28032867
  13. TIP60 complex regulates bivalent chromatin recognition/modification by 53BP1 through direct H4K20me binding and H2AK15 acetylation. PMID: 27153538
  14. Studies suggest that lysine (K) acetyltransferase inhibitors (KATi) are important for providing personalized therapies. PMID: 27528742
  15. Thus Tip60 interacts with RNR and NME3 to provide site-specific synthesis of dNTP for facilitating DNA repair in serum-deprived cells which contain low levels of dNTPs. PMID: 26945015
  16. TIP60 acted downstream of UHRF2 to regulate H3K9ac and H3K14ac expression. PMID: 27743347
  17. Data provide evidence that the acetylation of H2AX at Lys5 by TIP60 is required for the (ADPribosyl) ation activity and the dynamic binding of PARP-1 to chromatin after the induction of DNA damage. PMID: 26976643
  18. Synthetic lethality screening identifies TIP60-dependent radiation sensitivity in the absence of BAF180. PMID: 27461052
  19. E1A 243R promotes association of MYC/MAX with the NuA4/Tip60 complex, implicating the importance of the MYC/NuA4 pathway in cellular transformation by both MYC and E1A. PMID: 27664947
  20. UV irradiation enhanced the binding of ATF3 to Tip60, knockdown of ATF3 expression decreased Tip60 stability, thereby impairing Tip60 induction by UV irradiation. PMID: 26994140
  21. KAT5 is significantly elevated in malignant pleural mesothelioma PMID: 26780987
  22. Data suggest the combination of histone acetyltransferase TIP60 and microRNA miR-22 as prognostic indicator of breast cancer progression. PMID: 26512777
  23. Colony-formation assays and soft agar assays show that gain of function of TIP60 or depletion of EDD1 in HPV-positive cervical cancer cells significantly inhibits cell growth in vitro PMID: 26234678
  24. We demonstrate for the first time that tumor suppressor Tip60 down-regulates cell adhesion and MT1-MMP expression and thereby invasion of glioblastoma cells PMID: 26464124
  25. TIP60 relocalization to the chromatin to acetylates histone H4K16 and prevents the binding of 53BP1 to its docking site.Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia. PMID: 26446986
  26. Our results revealed a major role of the KAT5-ATM axis in protection of replicating chromatin against damage by the endogenous carcinogen FA. PMID: 26420831
  27. the acetylation-dependent NBS1 turnover by TIP60 on damaged chromatin restricts the dispersal of NBS1 foci from the sites of DNA damage. PMID: 26438602
  28. The stress-responsive gene ATF3 regulates the histone acetyltransferase Tip60 stability by promoting USP7-mediated deubiquitination of Tip60. PMID: 25865756
  29. Putative novel MYC interactors include components of the STAGA/KAT5 and SWI/SNF chromatin remodeling complexes PMID: 25452129
  30. TIP60 interacted with H3K4me3 in response to TNF-alpha signaling. PMID: 25560918
  31. Results establish that Tip60-T158 phosphorylation by p38 plays an essential role in stimulating Tip60 activity required for inducing the p53-PUMA pathway that ultimately leads to apoptosis in response to DNA damage. PMID: 25544752
  32. Tip60 is an important regulator of human papillomavirus genome amplification whose activity during the viral life cycle is controlled by STAT-5 and the kinase GSK3beta. PMID: 25673709
  33. HDAC3 promotes TIP60 ubiquitination and cytoplasmic localization and protects cells from apoptosis after DNA damage. PMID: 25301942
  34. These findings suggest that E2 recruits histone-modifying cellular proteins to the HPV LCR, resulting in transcriptional repression of E6 and E7. PMID: 25222147
  35. that KAT5 RNAi may result in cleaved casp9 upregulation through p38MAPK activation in Gallbladder carcinoma cells PMID: 24427328
  36. KAT5 and KAT6B regulate prostate cancer cell growth through PI3K-AKT signaling. PMID: 24294372
  37. Human melanoma patient samples and cell lines maintain p53 expression but PIASy and/or Tip60 are frequently lost. PMID: 23624367
  38. ZNF668 knockdown reduces Tip60-H2AX interaction and impairs ionizing radiation-induced histone H2AX hyperacetylation. PMID: 23777805
  39. degradation of Tip60 by the adenoviral early proteins is important for efficient viral early gene transcription and for changes in expression of cellular genes PMID: 23178490
  40. Tip60 differentially regulates the endogenous expression of the target genes by modulating the binding of ERbeta1 to the cis-regulatory regions. PMID: 23857583
  41. USP7 deubiquitinase activity is required for the stabilization of Tip60 in order to operate an effective p53-dependent apoptotic pathway in response to genotoxic stress. PMID: 23775119
  42. we conclude that PPAR agonists used in this work induces M1 macrophages polarization via inhibition of cPLA2 and the increase of aggressive microbicidal activity via reactive oxygen species (ROS) production. PMID: 23555077
  43. The role of Tip60 in mediating acetylation of p21 at its C-terminus is a novel and significant mechanism for post-translational regulation of cell-cycle progression. PMID: 23238566
  44. UHRF1 is a critical negative regulator of TIP60 and suggest that UHRF1-mediated effects on p53 may contribute, at least in part, to its role in tumorigenesis. PMID: 23677994
  45. study shows Tip60 plays an essential role in oncogenic ras-induced senescence; revealed a cascade of posttranslational modifications involving p38, Tip60 and PRAK, 3 proteins essential for ras-induced senescence; these modifications are critical for prosenescent function of Tip60 and PRAK PMID: 23685072
  46. Data indicate that ING5 associates with Tip60 (KAT5) to form a complex with p53. PMID: 23576563
  47. a novel pathway by which TIP60 and ThPOK synergistically suppresses Eomes function and IFNgamma production, which could contribute to the regulation of inflammation. PMID: 23609452
  48. RVBs are also required for heat stability of TIP60.com by a p400-independent pathway. PMID: 23297341
  49. tyrosine phosphorylation of KAT5 increases after DNA damage in a manner that promotes KAT5 binding to the histone mark H3K9me3; this triggers KAT5-mediated acetylation of the ATM kinase, promoting DNA-damage-checkpoint activation and cell survival PMID: 23708966
  50. Studies indicate histone acetyltransferase Tip60 as a potential therapeutic target for the treatment of prostate cancer. PMID: 23056207

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Subcellular Location Nucleus, Nucleus, nucleolus, Cytoplasm, perinuclear region
Protein Families MYST (SAS/MOZ) family
Database Links

HGNC: 5275

OMIM: 601409

KEGG: hsa:10524

STRING: 9606.ENSP00000340330

UniGene: Hs.397010

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