Recombinant Human Homeobox protein Hox-B5 (HOXB5)

Code CSB-YP010665HU
MSDS
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Source Yeast
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Code CSB-EP010665HU
MSDS
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Source E.coli
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Code CSB-EP010665HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010665HU
MSDS
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Source Baculovirus
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Code CSB-MP010665HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HOXB5
Uniprot No.
Alternative Names
HGNC:5116; HHO.C10; Homeo box 2A; Homeo box B5; Homeobox 2A; Homeobox B5; Homeobox protein HHO.C10; Homeobox protein Hox B5; Homeobox protein Hox-2A; Homeobox protein Hox-B5; Homeobox protein HoxB5; Homeobox protein Hu-1; Homeobox2A; HomeoboxB5; HOX 2; Hox 2.1; HOX 2A; HOX B5; HOX2; Hox2.1; Hox2.1, mouse, homolog of; HOX2A; HOXB 5; Hoxb5; HU 1; HU1; HXB5_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-269
Target Protein Sequence
MSSYFVNSFS GRYPNGPDYQ LLNYGSGSSL SGSYRDPAAM HTGSYGYNYN GMDLSVNRSS ASSSHFGAVG ESSRAFPAPA QEPRFRQAAS SCSLSSPESL PCTNGDSHGA KPSASSPSDQ ATSASSSANF TEIDEASASS EPEEAASQLS SPSLARAQPE PMATSTAAPE GQTPQIFPWM RKLHISHDMT GPDGKRARTA YTRYQTLELE KEFHFNRYLT RRRRIEIAHA LCLSERQIKI WFQNRRMKWK KDNKLKSMSL ATAGSAFQP
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Gene References into Functions
  1. for the first time we have shown that knockdown of HOXB5 significantly inhibited non-small cell lung cancer cell proliferation, invasion, metastasis, and epithelial-mesenchymal transformation, partly through the Wnt/beta-catenin signaling pathway PMID: 28337958
  2. Taken together, our results for the first time suggested that miR-455-3p was downregulated in NSCLC and was correlated with the poor prognosis of NSCLC patients. Also, miR-455-3p functions as tumor suppressor by directly targeting HOXB5 in NSCLC progression and may be used as a potential target for NSCLC treatment. PMID: 29170127
  3. HOXB5 acts as a positive modulator by promoting cell proliferative response and invasiveness in ER-positive breast cancer. PMID: 25999793
  4. HOXB5 may be an important regulator of the Wnt/beta-catenin signalling pathway, thereby contributing to gastric cancer progression and metastasis. PMID: 26467157
  5. Roles of Hoxb5 in the development of vagal and trunk neural crest cells. PMID: 25703667
  6. Overexpression of HoxB5 enhances blood vessel perfusion in vivo by up-regulation of MCP-1 and IL-6 as well as in enhanced leucocyte infiltration and blood vessel remodelling. PMID: 24189625
  7. HOXB5 and HOXB8 are frequently expressed in ovarian serous carcinoma, with anatomic site-related differences for cytoplasmic staining. HOXB5 may be affected by chemotherapy in effusions. PMID: 23438671
  8. The findings for HOXB5 and PHOX2B provide supportive evidence that genes regulating ENCC proliferation, migration and differentiation could be risk factors for Hirschsprung's disease PMID: 22648184
  9. A miRNA 7-binding single nucleotide polymorphism (1010A/G) located within 3'-UTR of HOXB5 is associated with gene expression and may be a promising prognostic factor for bladder cancer. PMID: 22768238
  10. HOXB5, HOXB6, and HOXB7 are activated in Barrett esophagus, and the midcluster HOXB gene signature in BE most resembled the colon rather than other GI epithelia. PMID: 22603795
  11. Single nucleotide polymorphism in HOXB5 gene is associated with childhood obesity. PMID: 22484627
  12. results support that expression of HOX genes is associated with oral squamous cell carcinoma; over 90% of OSCC samples presented HOXB5 expression versus 60% of non-tumor tissues, it can be suggested that HOXB5 may be related to the malignant phenotypes. PMID: 21552713
  13. Data show that human HOXB5 binds to the promoter region 5' upstream of the binding site of NKX2-1 and regulates RET expression. PMID: 21677782
  14. HoxB5 is necessary and sufficient to activate the cell-intrinsic events that regulate the differentiation of angioblasts and mature endothelial cells from their mesoderm-derived precursors PMID: 12897140
  15. The expression pattern of HOXB5 correlated with the migration and differentiation of neural crest cells. PMID: 12950074

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Subcellular Location
Nucleus.
Protein Families
Antp homeobox family
Tissue Specificity
Spinal cord.
Database Links

HGNC: 5116

OMIM: 142960

KEGG: hsa:3215

STRING: 9606.ENSP00000239151

UniGene: Hs.654456

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