Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Target Names

LY86

Uniprot No.

O95711

Research Area

Immunology

Alternative Names

(Ly-86)(Protein MD-1)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

21-162aa

Target Protein Sequence

GGGGKAWPTHVVCSDSGLEVLYQSCDPLQDFGFSVEKCSKQLKSNINIRFGIILREDIKELFLDLALMSQGSSVLNFSYPICEAALPKFSFCGRRKGEQIYYAGPVNNPEFTIPQGEYQVLLELYTEKRSTVACANATIMCS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

23.1 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 21-162 constitute the expression domain of recombinant Human LY86. The expected molecular weight for the LY86 protein is calculated to be 23.1 kDa. This LY86 protein is produced using e.coli expression system. The LY86 coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant LY86 protein in following stages of expression and purification.

Lymphocyte antigen 86 (LY86), also known as MD-1, is a protein involved in the regulation of immune responses. LY86 is a co-receptor for TLR4 and plays a crucial role in the recognition of bacterial lipopolysaccharides (LPS), triggering the activation of the immune system. As a part of the TLR4 signaling complex, LY86 contributes to the initiation of inflammatory responses by promoting the production of pro-inflammatory cytokines and type I interferons. Its involvement in these processes highlights its significance in the innate immune system's ability to detect and respond to bacterial infections.

Target Background

Function

May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression.

Gene References into Functions

our results reveal that MD-1 deficiency is of critical importance in down-regulating induction and progression of colitis, thereby suggesting that MD-1 might be a target for future interventional therapies of inflammatory bowel disease. PMID: 27209086
DNA methylation of the LY86 gene is associated with obesity, insulin resistance, and inflammation. PMID: 24735745
MD-1 may be a disease susceptibility gene for adult asthma in a Southern Han population in China. PMID: 21426727
Both mouse and human RP105/MD-1 exhibit dimerization of the 1:1 RP105/MD-1 complex, demonstrating a novel organization. PMID: 21959264
In this study, we produced variants of MD-1 and MD-2 in Pichia pastoris. Contrary to previous reports, this study suggests that MD-1 can bind to LPS. PMID: 21130168
in the promoter region we identified 3 SNPs, rs1334710, rs4959389 and rs977785 that are associated with mite-sensitive allergy in Taiwanese children; results suggested that MD-1 could be a susceptible gene for mite-sensitive allergy in Taiwanese children PMID: 18001295
Identified MD-1 SNP (rs7740529) association with asthma in Taiwanese children and adults. PMID: 19255686

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Subcellular Location

Secreted, extracellular space. Note=Associated with CD180 at the cell surface.

Tissue Specificity

Highly expressed in B-cells, monocytes and tonsil.

Database Links

HGNC: 16837

OMIM: 605241

KEGG: hsa:9450

STRING: 9606.ENSP00000230568

UniGene: Hs.653138

Code	CSB-EP013252HUb1
Abbreviation	Recombinant Human LY86 protein
MSDS	MSDS Datasheet
Size	$256
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Lymphocyte antigen 86 (LY86)

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Target Background

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