Recombinant Human NF-kappa-B inhibitor alpha(NFKBIA)

Code CSB-YP015761HU
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Source Yeast
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Code CSB-EP015761HU
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Source E.coli
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Code CSB-EP015761HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015761HU
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Source Baculovirus
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Code CSB-MP015761HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NFKBIA
Uniprot No. P25963
Alternative Names I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; IKBA_HUMAN; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha
Species Homo sapiens (Human)
Expression Region 1-317
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Gene References into Functions
  1. this study shows changes of NF-kB expression levels induced by cell-free DNA in different cell types PMID: 29743966
  2. the results of real-time PCR and western blotting revealed that Huaier extract decreased p65 and c-Met expression and increased IkappaBalpha expression, while paclitaxel increased p65 expression and reduced IkappaBalpha and c-Met expression.The molecular mechanisms may be involved in the inhibition of the NF-kappaB pathway and c-Met expression PMID: 29039556
  3. Colorectal cancer cases showed an increase in the frequency of NFkappaBIA-881G allele in Egyptian subjects. PMID: 28389768
  4. association between polymorphisms and progression of chronic hepatitis B Virus infection among Chinese Han Population PMID: 29093318
  5. miR-668 was upregulated in radioresistant human breast cancer cell lines MCF-7R and T-47DR, which targeted IkappaBalpha, activated the NF-kappaB pathway and thus increased the radioresistance of breast cancer cells. PMID: 28138801
  6. We showed that pristimerin suppressed tumor necrosis factor a (TNFalpha)-induced IkappaBa phosphorylation, translocation of p65, and expression of NFkappaB-dependent genes. Moreover, pristimerin decreased cell viability and clonogenic ability of Uveal melanoma (UM)cells. A synergistic effect was observed in the treatment of pristimerin combined with vinblastine, a frontline therapeutic agent, in UM. PMID: 28766683
  7. Data collectively demonstrate the functional importance of IkappaBalpha-mediated stripping of NFkappaB from DNA in the kinetic control of NFkappaB signaling. PMID: 28167786
  8. These findings indicate that genetic polymorphisms of NFKB1A rs696, pre-miR-146a rs2910164 and pre-miR-499 rs3746444 may represent novel markers of AT susceptibility. PMID: 28674224
  9. XPO1 inhibitor combination therapy with bortezomib or carfilzomib induces nuclear localization of IkappaBalpha and overcomes acquired proteasome inhibitor resistance in human multiple myeloma. PMID: 27806331
  10. Molecular docking analysis indicated that transcription factor NF-kappaB was one of the potential molecular targets modulated by DTTF. Specifically, the drug blocked the TNFalpha-induced phosphorylation of upstream IkappaBalpha kinase in a time-dependent manner leading to the suppression of NF-kappaB activation and nuclear translocation PMID: 27882436
  11. We show that HOTAIR regulates activation of NF-kB by decreasing Ik-Ba (NF-kB inhibitor) and establish that by inducing prolonged NF-kB activation and expression of NF-kB target genes during DNA damage, HOTAIR has a critical role in cellular senescence and platinum sensitivity PMID: 27041570
  12. Here, the authors report amide hydrogen/deuterium exchange data that reveal long-range allosteric changes in the NFkappaB (RelA-p50) heterodimer induced by DNA or IkappaBalpha binding. PMID: 28249778
  13. Sam68 is essential for DNA damage-induced NF-kappaB activation and colon tumorigenesis. PMID: 27458801
  14. Specifically, BCA2 serves as an E3 SUMO ligase in the SUMOylation of IkappaBalpha, which in turn enhances the sequestration of NF-kappaB components in the cytoplasm. Since HIV-1 utilizes NF-kappaB to promote proviral transcription, the BCA2-mediated inhibition of NF-kappaB significantly decreases the transcriptional activity of HIV-1. PMID: 28122985
  15. This study found that NFKBIA mRNAs was significantly expressed in normal tissues compared with glioma specimens. PMID: 27538656
  16. Findings indicate the prognostic value of NFKB inhibitor alpha (NFKBIA) in lower-grade gliomas (LGGs). PMID: 27052952
  17. W346 effectively inhibited tumor necrosis factor (TNF-a)-induced NF-kappaB activation by suppressing IKK phosphorylation, inhibiting IkB-a degradation, and restraining the accumulation of NF-kappaB subunit p65 nuclear translocation. W346 also affected NF-kappaB-regulated downstream products involved in cycle arrest and apoptosis. PMID: 26520440
  18. treating cells with SZC014 resulted in a decrease in phosphorylation of IkBa and NF-kappaB/p65 and NF-kappaB/p65 nuclear translocation. The cytotoxic activities of seven OA derivatives were generally stronger than that of OA, among which, SZC014 possessed the most potent anticancer activity in SGC7901 cells and would be a promising chemotherapic agent for the treatment of gastric cancer PMID: 26547583
  19. Network analysis identified NFKBIA as pathogenic gene in childhood asthma. PMID: 27420950
  20. HMBA was able to increase prostratin-induced phosphorylation and degradation of NF-kappaB inhibitor IkappaBalpha, thereby enhancing and prolonging prostratin-induced nuclear translocation of NF-kappaB, a prerequisite for stimulation of transcription initiation PMID: 27529070
  21. enhanced miR-381a-3p expression contributed to the injury of osteoarthritis mainly by inhibiting the expression of IkappaBalpha. PMID: 27312547
  22. Timely and efficient degradation of ubiquitinated IkappaB[alpha], concomitant with timely and efficient liberation of RelA from ubiquitinated IkappaB[alpha] and RelA nuclear translocation, essentially depends on the presence of functional p97/VCP. PMID: 26463447
  23. Activated Rac1 regulates the degradation of IkappaBalpha and the nuclear translocation of STAT3-NFkappaB complexes in starved cancer cells PMID: 27151455
  24. mutation in Chinese patient results in mycobacterial infections without ectodermal dysplasia PMID: 26691317
  25. DAT stabilized IkBa by inhibiting the phosphorylation of Ika by the IkB kinase (IKK) complex. DAT induced proteasomal degradation of TRAF6, and DAT suppressed IKKb-phosphorylation through downregulation of TRAF6 PMID: 26647777
  26. the rs3138053 polymorphism of NFKBIA gene is a candidate for susceptibility to overall cancers, while rs696 plays a protective role [meta-analysis] PMID: 26488500
  27. Identify a novel BCR-ABL/IkappaBalpha/p53 network, whereby BCR-ABL functionally inactivates a key tumor suppressor in chronic myeloid leukemia. PMID: 26295305
  28. Study demonstrates an association between functional polymorphisms of IkappaBalpha rs696 and smoking with the risk of defective spermatogenesis, suggesting some interaction between the NF-kappaB signalling pathway and smoking-related ROS in human spermatogenesis. PMID: 25352423
  29. genetic variation associated with susceptibility to acute kidney injury PMID: 26477820
  30. MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of IkappaBalpha. PMID: 26239140
  31. No association was observed between NFKBIA variants and risk of liver cancer. PMID: 24578542
  32. SM22alpha is a phosphorylation-regulated suppressor of IKK-IkappaBalpha-NF-kappaB signaling cascades. PMID: 25937534
  33. Data suggest that activity of IKBalpha can be regulated by dietary factors; dietary supplementation with luteolin inhibits vascular endothelial inflammation by suppressing IKBalpha/NFkappaB signaling. PMID: 25577468
  34. This review and meta-analysis of the association of NFKBIA -881 A>G polymorphism with cancer susceptibility reveals that -881 A>G polymorphism may increase risk of cancer development in Asian populations. PMID: 26252270
  35. miR-126 may play an important role in hepatic fibrosis by downregulating the expression of IkappaBalpha partly through the NF-kappaB signaling pathway. PMID: 25974152
  36. IkappaBetaalpha inhibits apoptosis at the outer mitochondrial membrane independently of NF-kappaB retention. PMID: 25361605
  37. The single nucleotide polymorphism rs1957106 CT and TT genotypes were found to be associated with lower NFKBIA protein levels and a poor prognosis of pateints with glioblastoma. PMID: 25215581
  38. data suggest that the NFKBIA 126 G/A polymorphism might be potentially helpful to identify liver transplant recipients with an increased susceptibility to develop recurrent acute rejections PMID: 25112903
  39. Expression of IkappaBalpha in human bladder cancer cells is negatively correlated with epithelial-mesenchymal transition and tumor invasion in vitro. PMID: 25374080
  40. NFKBIA-rs2233419AG was associated with a significantly increased risk of developing recurrent wheezing. PMID: 25326706
  41. miR-196a can directly interact with IkappaBalpha 3'-UTR to suppress IkappaBalpha expression and subsequently promote activation of NF-kappaB PMID: 24463357
  42. MiR-196a promotes pancreatic cancer progression by targeting nuclear factor kappa-B-inhibitor alpha. PMID: 24504166
  43. Data indicate that following bortezomib treatment there was accumulation of IkappaB-alpha (IkappaBalpha) without affecting its phosphorylation status at an early time point. PMID: 23697845
  44. This study reveals that polymorphisms in the IkB-alpha promoter (-881 A/G, -826 C/T) are strongly associated with the susceptibility of Iranian Multiple Sclerosis patients PMID: 24368589
  45. The results of this study suggested that oligodendroglial IkappaBalpha expression and NF-kappaB are activated early in the course of MSA and their balance contributes to the decision of cellular demise. PMID: 24361600
  46. No statistically significant CRC risk association was found for the NFKBIA polymorphisms. PMID: 23996241
  47. data indicate that NFKBIA deletions are present but not frequent in Glioblastoma multiforme (GBM); the deletions become frequent in GBM neurospheres (NS), an event that seems to be affected by the presence of EGF in the culture medium PMID: 24330732
  48. the analysis of IkappaBalpha expression at salivary gland epithelial cell level could be a potential new hallmark of Sjogren's syndrome progression. PMID: 23377923
  49. IkappaBalpha sequesters not only p65 but also RPS3 in the cytoplasm. PMID: 24457201
  50. NF-kB expression was downregulated and its cytoplasmatic inhibitor IKBalpha was increased in CTLA4-Ig treated macrophages. PMID: 24295474

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Involvement in disease Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant (ADEDAID)
Subcellular Location Cytoplasm. Nucleus.
Protein Families NF-kappa-B inhibitor family
Database Links

HGNC: 7797

OMIM: 164008

KEGG: hsa:4792

STRING: 9606.ENSP00000216797

UniGene: Hs.81328

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