Code | CSB-RA015761A32phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IF | 1:20-1:200 |
The production of the phospho-NFKBIA (S32) recombinant monoclonal antibody starts with the isolation of genes responsible for encoding this antibody from rabbits previously immunized with a synthesized peptide derived from the human NFKBIA protein phosphorylated at S32. These antibody genes are then meticulously cloned into specialized expression vectors. Following this genetic modification, the modified vectors are introduced into host suspension cells, which are carefully cultured to stimulate the expression and secretion of antibodies. After this cultivation phase, the phospho-NFKBIA (S32) recombinant monoclonal antibody is subjected to a rigorous purification process utilizing affinity chromatography techniques, effectively separating the antibody from the surrounding cell culture supernatant. Finally, the functionality of the antibody is comprehensively assessed through a diverse range of assays, including ELISA, WB, and IF tests, conclusively confirming its ability to interact effectively with the human NFKBIA protein phosphorylated at S32.
Phosphorylation of NFKBIA at S32 is a critical regulatory event in the NF-κB signaling pathway, controlling the activation of NF-κB and influencing various cellular processes, including immune responses, inflammation, and cell survival. Dysregulation of this phosphorylation event can have significant implications in inflammatory diseases, cancer, and other pathological conditions.
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