Phospho-NFKBIA (S32) Antibody

Datasheet
Code CSB-RA015761A32phHU
Size US$350
Uniprot No. P25963
Image
  • Western Blot
    Positive WB detected in:A549 whole cell lysate(treated with Calyculin A or not)
    All lanes:Phospho-NFKBIA antibody at 1.07μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 39 KDa
    Observed band size: 39 KDa

  • Immunofluorescence staining of A549 cells with CSB-RA015761A32phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen A synthesized peptide derived from Human Phospho-NFKBIA (S32)
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200
Relevance Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Gene Names NFKBIA
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Involvement in disease Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant (ADEDAID)
Subcellular Location Cytoplasm, Nucleus
Protein Families NF-kappa-B inhibitor family
Database Links

HGNC: 7797

OMIM: 164008

KEGG: hsa:4792

STRING: 9606.ENSP00000216797

UniGene: Hs.81328

Pathway cAMP signaling pathway
Chemokine signaling pathway
NF-kappa B signaling pathway
TNF signaling pathway
Apoptosis
B cell receptor signaling pathway
IL-17 signaling pathway
Inflammasome Signaling
NOD-like receptor signaling pathway
Osteoclast differentiation
T cell receptor signaling pathway
Th1 and Th2 cell differentiation
Th17 cell differentiation
Toll-like receptor signaling pathway
Adipocytokine signaling pathway
Neurotrophin signaling pathway

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