Recombinant Human Neuronal calcium sensor 1(NCS1)

Code CSB-EP008983HU
Size US$1726
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names NCS1
Uniprot No. P62166
Research Area Neuroscience
Alternative Names 9430075O15Rik; A730032G13Rik; AI836659; DKFZp761L1223; FLUP; FREQ; Frequenin; Frequenin homolog (Drosophila); Frequenin homolog; Frequenin like protein; Frequenin, Drosophila, homolog of; Frequenin-like protein; Frequenin-like ubiquitous protein; Mfreq; NCS 1; NCS-1; ncs1; NCS1_HUMAN; Neuronal calcium sensor 1; Neuronal Calicum Sensor 1
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-190aa
Target Protein Sequence GKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 48.7kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity).
Gene References into Functions
  1. NCS-1, a calcium-binding protein, is associated with clinicopathologic features of aggressiveness in breast cancer cells and worse outcome in two breast cancer patient cohorts PMID: 28275088
  2. Results show that although NCS-1 Var2mRNA can be detected, protein expression was not detectable. When overexpressed in cell lines NCS-1 Var2 appears to be folded properly, yet it has a lower calcium affinity. Because most NCS-1 binding to its protein partners requires calcium binding, it is unlikely that NCS-1 Var2 would play a major regulatory role in cells. PMID: 27575489
  3. structural and dynamical response of NCS-1 protein to elevated temperature may be one of its intrinsic functional properties PMID: 27007011
  4. Lithium normalizes gamma band oscillations mediated by P/Q-type calcium channels modulated by NCS-1. PMID: 27033453
  5. Study determined the salient features of the folding free-energy landscapes of the Mg2+-bound and apo states of NCS-1 PMID: 26153708
  6. results reveal the important role of salt bridges on the structural properties of NCS-1 protein and that R102Q mutation disables the dynamic relocation of C-terminus, which may block the binding of NCS-1 protein to its receptors PMID: 25343687
  7. single-molecule optical tweezers were used to monitor misfolding reactions of the human neuronal calcium sensor-1. PMID: 25157171
  8. A strong liason was characterized between myristoylation and cryptic EF-1 in NCS-1. PMID: 25565019
  9. The functional link in substantia nigra dopamine neurons between Cav1.3- L-type-Ca2+ channels and D2-autoreceptor activity is an adaptive signalling network controlled by NCS-1. PMID: 24934288
  10. NCS1 has evolved a remarkable complex interdomain cooperativity and a fundamentally different folding mechanism compared to structurally related proteins. PMID: 24012477
  11. data suggest that genetic variants in the NCS-1 gene contribute to susceptibility of Cocaine Abuse in individuals of African descent. PMID: 22999924
  12. [review] Receptor interaction properties in schizophrenia are dependent upon NCS-1, a protein found to regulate the phosphorylation, trafficking, and signaling profile of the D2 dopamine receptor (D2R) in neurons. PMID: 21777187
  13. the C-terminal tail is important for regulating the conformational stability of NCS1 in Ca(2+)-activated state PMID: 22227393
  14. analysis of the interaction between the D2 dopamine receptor and neuronal calcium sensor-1 PMID: 21875085
  15. it is possible to protect cells from paclitaxel-induced degradation of NCS-1 by inhibiting calpain activity PMID: 21808066
  16. NCS-1 is a novel regulator of cardiac calcium signaling, specifically in immature and hypertrophic hearts. PMID: 21737792
  17. Impairment of the normal cycling of NCS-1 by the R102Q mutation could have subtle effects on neuronal signalling and physiology in the developing and adult brain. PMID: 20479890
  18. up-regulated in the prefrontal cortex of schizophrenic and bipolar patients PMID: 12496348
  19. Analysis with chimeric proteins between KChIP2 and NCS-1 reveals that the three regions of KChIP2 are necessary and sufficient for its effective binding to Kv4.3 protein PMID: 12928444
  20. may serve as regulator of certain isoforms of phosphatidylinositol 4-kinase PMID: 14512421
  21. key role for residues within the motif EELTRK in NCS-1 in keeping the myristoyl group exposed and allowing the protein to be constitutively membrane-associated. PMID: 14726528
  22. NCS-1 is shown to be mainly associated with azurophilic granules in human neutrophils and promyelocitic cells and, therefore could play an instrumental role in the calcium-dependent secretion of azurophilic granules PMID: 14760944
  23. These results demonstrate a novel role for NCS-1 and PI4Kbeta in regulating ERK1/2 signaling and inflammatory reactions in mast cells. PMID: 16837555
  24. NCS-1 expression was diminished in CD4+T lymphocytes, CD19+ B lymphocytes and CD14+ monocytes of bipolar disorder patients and also decreased in CD4+ T lymphocytes and CD56+ natural killer cells of schizophrenia patients PMID: 19091302
  25. occurrence of an unusual TG 3' splice site in intron 2 PMID: 17672918

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Subcellular Location Golgi apparatus, Cell junction, synapse, postsynaptic cell membrane, postsynaptic density, Cytoplasm, perinuclear region, Cytoplasm, Cell membrane, Peripheral membrane protein, Membrane, Lipid-anchor
Protein Families Recoverin family
Database Links

HGNC: 3953

OMIM: 603315

KEGG: hsa:23413

STRING: 9606.ENSP00000361475

UniGene: Hs.642946

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