Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
alpha 1; DEF1; DEF1_HUMAN; DEFA1; DEFA1B; DEFA2; Defensin 1; Defensin; Defensin; alpha 1; Defensin; alpha 1; myeloid related sequence; Defensin; alpha 2; HNP-1; HNP-2; HNP1; HP-1; HP-2; HP1; HP2; MRS; Myeloid related sequence; Neutrophil defensin 1; Neutrophil defensin 2
Species
Homo sapiens (Human)
Target Protein Sequence
DIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Human DEFA1 covers amino acids 1-94. This DEFA1 protein is theoretically predicted to have a molecular weight of 37.2 kDa. Expression of this DEFA1 protein is conducted in e.coli. The DEFA1 gene fragment has been modified by fusing the N-terminal GST tag, providing convenience in detecting and purifying the recombinant DEFA1 protein during the following stages.
Neutrophil defensin 1 (DEFA1) is a member of the defensin family, small cationic peptides with antimicrobial properties. DEFA1 is primarily produced by neutrophils, a type of white blood cell, and serves as part of the innate immune system's defense against microbial infections. This antimicrobial peptide exerts its function by disrupting the microbial cell membrane, leading to cell death. Beyond its antimicrobial role, DEFA1 has been implicated in various immune responses and inflammatory processes. Research areas related to DEFA1 include investigations into its role in host-pathogen interactions, immune regulation, and potential therapeutic applications in combating infectious diseases. Understanding the functions of DEFA1 contributes to insights into the immune system's defense mechanisms.