Recombinant Human Nuclear receptor corepressor 2(NCOR2) ,partial

Code CSB-YP897303HU
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Source Yeast
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Code CSB-EP897303HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP897303HU
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Source Baculovirus
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Code CSB-MP897303HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NCOR2
Uniprot No. Q9Y618
Alternative Names CTG 26; CTG repeat protein 26; CTG26; N CoR2; N-CoR2; NCoR 2; Ncor2; NCOR2_HUMAN; Nuclear Receptor Co-Repressor 2; Nuclear receptor corepressor 2; retinoic-acid-receptor-associated corepressor; Silencing Mediator for Retanoid and Thyroid Hormone Receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; SMAP 270; SMAP270; SMRT; SMRTE; SMRTE tau; T3 receptor associating factor; T3 receptor-associating factor; Thyroid retinoic acid receptor associated corepressor; Thyroid-; TNRC 14; TNRC14; TRAC 1; TRAC; TRAC1
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Transcriptional corepressor. Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 4 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation.
Gene References into Functions
  1. this report tried to address the molecular basis for the direct interaction between CSL and SMRT. PMID: 30157580
  2. The pregnane X receptor (PXR) and the nuclear receptor corepressor 2 (NCoR2) modulate cell growth in head and neck squamous cell carcinoma PMID: 29470550
  3. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. PMID: 29262348
  4. this study identifies NCOR2 as a new gene for FVC, indicating the importance of further research into the role of vitamin A intake/supplementation and its interactions with related genes in the regulation of FVC. PMID: 26836265
  5. Molecular basis for the specific interactions between HDAC4 and the SMRT corepressor. PMID: 26161557
  6. SNPs in Notch pathway genes may be predictors of cutaneous melanoma disease-specific survival. PMID: 25953768
  7. Significant methylation changes in the SLC23A2 and NCOR2 regulatory regions. PMID: 25821969
  8. A repeated peptide motif present in both SMRT and NCoR is sufficient to mediate specific interaction, with micromolar affinity, with all the Class IIa Histone Deacetylases (HDACs 4, 5, 7, and 9). PMID: 26055705
  9. SMRT enhances cell growth of estrogen receptor-alpha-positive breast cancer cells by promotion of cell cycle progression and inhibition of apoptosis. PMID: 24971610
  10. Phosphorylation of the CK2 site on SMRT significantly increased affinity for SHARP. PMID: 24268649
  11. SMRT is responsible for basal repression of Wip1,a phosphatase that de-phosphorylates and inactivates Chk2, thus affecting a feedback loop responsible for licensing the correct timing of Chk2 activation and the proper execution of the DNA repair process. PMID: 23690919
  12. SMRT may be recruited in the SXR-cofactor complex even in the presence of ligand; SMRTmay be involved not only in SXR-mediated suppression without ligand, but also in ligand-activated transcription to suppress the overactivation of transcription PMID: 23562850
  13. There was no association between SMRT expression and overall survival for patients, regardless of whether they received tamoxifen PMID: 23015261
  14. expression of the transcriptional corepressor complex subunits GPS2 and SMRT was significantly reduced in obese adipose tissue, inversely correlated to inflammatory status PMID: 23221346
  15. Both MEK1 and SMRT bind to the c-Fos promoter and regulate its transcription; SMRT knockdown results in early-phase stimulation followed by a late-phase inhibition of T cell activation. PMID: 23225884
  16. Thyroid hormone receptors induce TRAIL expression, and TRAIL thus synthesized acts in concert with simultaneously synthesized Bcl-xL to promote metastasis PMID: 22576662
  17. Our findings highlight a novel splice variant of NCOR2 as a candidate biomarker in breast cancer that not only predicts tamoxifen response but may be targeted to overcome tamoxifen resistance PMID: 23117886
  18. SMRT and GR act in a consistent manner with steroid hormones PMID: 23055525
  19. altered recruitment and loss of corepressors SMRT/NCoR may provide a mechanism that changes the response of AR function to ligands and contributes to the progression of the advanced stages of human prostate cancer. PMID: 22695118
  20. miR-16 targets SMRT and modulates NF-kappaB-regulated transactivation of the IL-8 gene. PMID: 22292036
  21. An equilibrium dissociation constant obtained for SMRT in the presence and absence of rifampicin indicates that the ligand does not enhance the affinity of the pregnane X receptor or its corepressor. PMID: 22185585
  22. Data report that SMRT interacts with itself to form a protein dimer, and that Erk2, a mitogen-activated protein (MAP) kinase, disrupts this SMRT self-dimerization in vitro and in vivo. PMID: 20965228
  23. The crystal structure of the tetrameric oligomerization domain of TBL1, which interacts with both SMRT and GPS2, and the NMR structure of the interface complex between GPS2 and SMRT. PMID: 21240272
  24. This study independently verifies the influence of NCOR2 and IDH1 on HIV transmission, and its findings suggest that variation in these genes affects susceptibility to HIV infection in exposed individuals. PMID: 21083371
  25. Aberrant expression and modification of SMRT might be involved in the pathogenesis of tumoral cortisol resistance. PMID: 20555024
  26. Tamoxifen treatment of breast cancer cells reduced the expression of ER-alpha and increased the expression of SMRT. PMID: 18546531
  27. SMRT decreased with oestradiol treatment in human skeletal muscle cells PMID: 19432593
  28. Sstable binding of the Stat5-RARalpha fusion protein to corepressor SMRT is accompanied by an impaired response to differentiation signals in hematopoietic cells PMID: 11929749
  29. Interactions that determine the assembly of a retinoid X receptor/corepressor complex PMID: 11972046
  30. The silencing mediator of retinoic acid and thyroid hormone receptors can interact with the aryl hydrocarbon (Ah) receptor but fails to repress Ah receptor-dependent gene expression. PMID: 12139968
  31. SMRT has a role as a coactivator for thyroid hormone receptor T3Ralpha from a negative hormone response element PMID: 12388540
  32. a significant role of SMRT in modulating androgen receptor transcriptional activity PMID: 12441355
  33. Differing transcriptional properties appear to reflect the differing abilities of the three RAR isotypes to interact with the SMRT corepressor protein. PMID: 12554770
  34. SMRT and DAX-1 repress agonist-dependent activity of both androgen and progesterone receptors PMID: 12771131
  35. SANT motif interprets the histone code and promotes histone deacetylation PMID: 12840002
  36. Data describe a 17 residue fragment from SMRT that binds to the BCL6 BTB domain, and report the crystal structure of the complex to 2.2 angstroms. PMID: 14690607
  37. Elevated SMRT levels are common in prostate cancer cells, resulting in suppression of target genes associated with antiproliferative action. PMID: 15300237
  38. SMRTbeta expression may influence the binding and transcriptional capacities of nuclear receptors in tumor cells (SMRTbeta) PMID: 15319284
  39. differential mRNA splicing of SMRT serves to customize corepressor function in different cells, allowing the transcriptional properties of nuclear receptors to be adapted to different contexts PMID: 15632172
  40. No significant allelic/genotypic association between any of the five mutations in SMRT/N-CoR2 and bipolar phenotype and the CAG repeat did not demonstrate allelic instability. PMID: 15635693
  41. the subnuclear positioning of SMRT is influenced by the ligand-bound ERalpha, and this activity is dependent on the ratio of the co-expressed ERalpha and SMRT PMID: 15713534
  42. TRAC-1 (T cell RING protein identified in activation screen)is the first E3 ubiquitin ligase that serves a positive regulatory role in T cell activation. PMID: 15843525
  43. The aryl hydrocarbon receptor activates the retinoic acid receptoralpha through SMRT antagonism PMID: 16480812
  44. expression of the silencing mediator of retinoid and thyroid receptor (SMRT) & histone deacetylase4 (HDAC4) enhances formation of Bach2 foci in nuclear matrix. SMRT mediates HDAC4 binding to Bach2, & HDAC4 facilitates retention of Bach2 in the foci. PMID: 17383980
  45. NCOR2/SMRT is associated with poor patient outcome in breast cancer PMID: 17902051
  46. These results would shed new insights for the molecular mechanisms of PML-RARalpha-associated leukemogenesis. PMID: 17991421
  47. CD40 signaling rapidly disrupts the ability of BCL6 to recruit the SMRT corepressor complex by excluding it from the nucleus, leading to histone acetylation, RNA polymerase II processivity, and activation of BCL6 target genes PMID: 18487509
  48. SMRT protein stability is regulated by Pin1 and Cdk2. PMID: 18838553
  49. Activated MEK signaling cascade inhibits functional recruitment of corepressor SMRT to cyproterone acetate-bound AR in prostate cancer cells. PMID: 19223455
  50. SMRT function restoration induces JAG2 down-regulation as well as multipe myeloma apoptosis. PMID: 19417136

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Subcellular Location Nucleus.
Protein Families N-CoR nuclear receptor corepressors family
Tissue Specificity Ubiquitous. High levels of expression are detected in lung, spleen and brain.
Database Links

HGNC: 7673

OMIM: 600848

KEGG: hsa:9612

STRING: 9606.ENSP00000384018

UniGene: Hs.137510

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