Recombinant Human PR domain zinc finger protein 2 (PRDM2), partial

Code CSB-YP613379HU
MSDS
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Source Yeast
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Code CSB-EP613379HU
MSDS
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Source E.coli
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Code CSB-EP613379HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP613379HU
MSDS
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Source Baculovirus
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Code CSB-MP613379HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PRDM2
Uniprot No.
Alternative Names
GATA-3-binding protein G3B; HUMHOXY1; KMT8; Lysine N-methyltransferase 8; MTB-ZF; MTE-binding protein; PR domain containing 2; with ZNF domain; PR domain zinc finger protein 2; PR domain-containing protein 2; PRDM2; PRDM2_HUMAN; Retinoblastoma protein binding zinc finger protein; Retinoblastoma protein-interacting zinc finger protein; Retinoblastoma protein-interacting zinc-finger protein; RIZ; RIZ1; RIZ2; Zinc finger DNA binding protein; Zinc finger protein RIZ
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
Gene References into Functions
  1. The authors have found that overexpression of RIZ1 in HEK293 cells reduced the expression of Akt3 protein. PMID: 29367689
  2. Promoter hypermethylation may play an important role in the epigenetic silencing of RIZ1 expression in human glioma tissues and glioblastoma cell lines. PMID: 27757741
  3. the Pro704 p-/- allele of RIZ is significantly more common in CML patients with Loss of hematological response than good responders (p = .001). We showed that LOH p-/- homozygosity of RIZ1 was common event in BC than proline p+/+ homozygosity and proline p+/+ homozygosity more common feature in CP phase CML. PMID: 27830966
  4. Promoter region methylation of RIZ1 may play an important role in the epigenetic silencing of RIZ1 expression in pituitary adenomas, which may translate into important diagnostic and therapeutic applications PMID: 28718376
  5. RIZ1 expression is negatively correlated with glioma differentiation and can serve as a predictor of glioma prognosis and thus could be a potential therapeutic target for patients with gliomas. PMID: 26690953
  6. Loss of RIZ1 expression due to methylation is associated with Renal Cell Carcinoma. PMID: 25987089
  7. PRDM2 downregulation may play a role in dopamine-agonist resistance and tumor recurrence in prolactinomas. PMID: 25884948
  8. Results suggest that high expression of SMYD3 is related to the occurrence of esophageal squamous cell carcinoma. Also, its suppression promoted the expression of RIZ1 suggesting a signal transduction pathway between SMYD3 and RIZ1. PMID: 24993551
  9. PRDM2, PRDM5, PRDM16 promoters are methylated and their expression is suppressed in lung cancer cells. PMID: 24966940
  10. The development and progression of esophageal squamous cell carcinoma are related to the inactivation of RIZ1. PMID: 24115813
  11. RIZ1 expression is significantly downregulated as the formation of meningiomas progressed, and suggest that RIZ1 may represent a promising candidate tumor suppressor gene that contributes to malignant meningiomas. PMID: 22614009
  12. Reduction of RIZ1 expression aggravates the progression of liver fluke-related cholangiocarcinoma PMID: 23098508
  13. Aberrant methylation and decreased expression of the RIZ1 gene are frequent in adult acute lymphoblastic leukemia of T-cell phenotype PMID: 22300346
  14. Data suggest that promoter methylation may play an important role in the epigenetic silencing of RIZ1 gene expression in human esophageal squamous cell carcinoma. PMID: 22363126
  15. Acting as a negative regulator of RIZ1 function, PRFM2 mediates effect of cell proliferation by estrogen via regulation of cell survival and differentiating gene expression. PMID: 21503890
  16. RIZ1 may be a potential tumor suppressor in human esophageal squamous cell cancer. PMID: 22372344
  17. RIZ1 might play an important role in tumor metastasis, and the PR domain alone possessed anticancer activity. PMID: 21369371
  18. Results suggest that the RIZ1 gene is inactivated in MDS and AML in part by methylation, whereas another mechanism should be involved in others. PMID: 20828818
  19. promoter methylation and H3K9 modifications work together to silence the RIZ1 gene in hepatocellular carcinoma (HCC). PMID: 20675009
  20. RIZ1 promoter methylation is not a common event in neuroblastoma. PMID: 20878080
  21. RIZ1 negatively regulated the cell proliferation of monocytic leukemia cells via activation of p53. PMID: 20594067
  22. Loss of RIZ1 expression will lead to an increase in myeloid blast cell population resulting in CML progression. PMID: 19602237
  23. Reduced expression of RIZ1 may play an important role in the pathogenesis and/or development of cervical cancer, and is considered to be caused in part by aberrant DNA methylation PMID: 20159667
  24. loss of RIZ1 mRNA in human cancers is associated with DNA methylation of its promoter CpG island. PMID: 11719434
  25. These findings suggest that RIZ may be a possible target of structural alteration leading to leukemia. PMID: 12002276
  26. Frameshift mutations of RIZ, but no point mutations, were found in RIZ1 exons in malignant melanomas with deletions in 1p36. RIZ has potential role in the multi-step tumor forming process of malignant melanoma of the skin. PMID: 12082534
  27. RIZ1 in gastric neoplasms undergoes biallelic inactivation. PMID: 14534544
  28. Tumor suppressor RIZ1 (PRDM2) methylates histone H3 on lysine 9, and this activity is reduced by mutations in the PR domain found in human cancers. PMID: 14633678
  29. Frameshift mutations of RIZ may play an important role in gastric cancers with microsatellite instability. PMID: 15309726
  30. the presented results indicated that the Zn-finger domain could be endowed with the putative oncogenic activity of RIZ2 gene product. PMID: 15488642
  31. RIZ1 may be a new candidate gene for involvement in the variation seen in bone mineral density. PMID: 15579774
  32. recurrent inactivation of the tumour suppressor RIZ1 suggests that this event may be a significant contributing factor to tumour development in pheochromocytomas and abdominal paragangliomas PMID: 15809732
  33. Forced RIZ1 expression in CML-BC cell lines decreases IGF-1 receptor activation and activation of downstream signaling components extracellular signal-regulated kinase 1/2 and AKT PMID: 16953217
  34. The current study suggested an important role for RIZ1 expression in thyroid tumorigenesis. PMID: 17103461
  35. A563G variant not found in diffuse large B-cell lymphomas. PMID: 17205536
  36. the ERalpha cofactor RIZ gene has a prominent effect on bone marrow density(BMD), and the P704 genotype modulates the impact of estradiol on BMD PMID: 17356055
  37. RIZ polymorphisms may be important predictive markers for lung cancer susceptibility. PMID: 17693662
  38. Reduced expression of RIZ1 may play an important role in the pathogenesis and/or development of epithelial ovarian carcinoma, and is considered to be caused in part by aberrant DNA methylation. PMID: 17922684
  39. The proliferation, migration induction and apoptosis inhibition activities of SMYD3 in hepatocellular carcinoma may be mediated through RIZ1 CpG promoter hypermethylation. PMID: 17963297
  40. observed no association of the RIZ1 Pro704 insertion-deletion polymorphism with BMD or fracture risk PMID: 18037365
  41. Results suggest that the inactivation of the RIZ1 by DNA methylation at its promoter region is involved in the tumorigenesis of hepatocellular carcinoma, particularly in the early stage of disease. PMID: 18712668
  42. methyl-balanced diet conferred additional survival benefits compared to a tumor-inducing methyl-imbalanced diet only in mice with wild type RIZ1 but not in mice deficient in RIZ1 PMID: 18852888
  43. RIZ1 is expressed in normal prostate epithelial cells and down-regulated in cancer cells, with a switch of its localization from the nucleus to the cytoplasm upon cancer grade progression. RIZ1 levels were modulated by DHT or E2 treatment in vitro. PMID: 19746436

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Subcellular Location
Nucleus.
Protein Families
Class V-like SAM-binding methyltransferase superfamily
Tissue Specificity
Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
Database Links

HGNC: 9347

OMIM: 601196

KEGG: hsa:7799

STRING: 9606.ENSP00000235372

UniGene: Hs.371823

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