Recombinant Human Peroxisomal multifunctional enzyme type 2(HSD17B4)

Code CSB-YP010774HU
Size US$1298
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names HSD17B4
Uniprot No. P51659
Research Area Cancer
Alternative Names 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; 17 beta HSD 4; 17 beta HSD IV; 17 beta hydroxysteroid dehydrogenase 4; 17-beta-HSD 4; 17-beta-hydroxysteroid dehydrogenase 4; 17beta estradiol dehydrogenase type IV; 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase; 3-alpha; 7-alpha; Beta hydroxyacyl dehydrogenase; Beta keto reductase; D 3 hydroxyacyl CoA dehydratase; D bifunctional protein; D bifunctional protein peroxisomal ; D-3-hydroxyacyl CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein; D-bifunctional protein; D-bifunctional protein, peroxisomal; DBP; DBP, peroxisomal; DHB4_HUMAN; EDH17B4; Enoyl-CoA hydratase 2; Hsd17b4; Hydroxysteroid (17-beta) Dehydrogenase 4; MFE 2; MFE-2; MPF-2; Multifunctional protein 2; Peroxisomal multifunctional enzyme type 2; Peroxisomal multifunctional protein 2; PRLTS1; SDR8C1; Short chain dehydrogenase/reductase family 8C member 1
Species Homo sapiens (Human)
Source Yeast
Expression Region 1-736aa
Target Protein Sequence MGSPLRFDGRVVLVTGAGAGLGRAYALAFAERGALVVVNDLGGDFKGVGKGSLAADKVVEEIRRRGGKAVANYDSVEEGEKVVKTALDAFGRIDVVVNNAGILRDRSFARISDEDWDIIHRVHLRGSFQVTRAAWEHMKKQKYGRIIMTSSASGIYGNFGQANYSAAKLGLLGLANSLAIEGRKSNIHCNTIAPNAGSRMTQTVMPEDLVEALKPEYVAPLVLWLCHESCEENGGLFEVGAGWIGKLRWERTLGAIVRQKNHPMTPEAVKANWKKICDFENASKPQSIQESTGSIIEVLSKIDSEGGVSANHTSRATSTATSGFAGAIGQKLPPFSYAYTELEAIMYALGVGASIKDPKDLKFIYEGSSDFSCLPTFGVIIGQKSMMGGGLAEIPGLSINFAKVLHGEQYLELYKPLPRAGKLKCEAVVADVLDKGSGVVIIMDVYSYSEKELICHNQFSLFLVGSGGFGGKRTSDKVKVAVAIPNRPPDAVLTDTTSLNQAALYRLSGDWNPLHIDPNFASLAGFDKPILHGLCTFGFSARRVLQQFADNDVSRFKAIKARFAKPVYPGQTLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDLAPTSGTSAKTPSEGGKLQSTFVFEEIGRRLKDIGPEVVKKVNAVFEWHITKGGNIGAKWTIDLKSGSGKVYQGPAKGAADTTIILSDEDFMEVVLGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAKL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 81.7kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.
Gene References into Functions
  1. This study reveals a crosstalk between acetylation and chaperone-mediated autophagy degradation in HSD17B4 regulation. PMID: 28296597
  2. we identified that methylation of the promoter CpG island of HSD17B4 was associated with the pathological complete response of HER2-positive breast cancer to trastuzumab and chemotherapy with a specificity of 79% PMID: 28186977
  3. Our findings supported that HSD17B4 was one of the genes contributing to Perrault syndrome in this consanguineous Chinese Han family. PMID: 28830375
  4. ere we present eight families affected by Perrault syndrome. In five families we identified novel or previously reported variants in HSD17B4, LARS2, CLPP and C10orf2 PMID: 26970254
  5. Results show that HSD17B4 is highly expressed in hepatocellular carcinoma (HCC) cells and activated NF-kappaB co-localized with the NF-kappaB-responsive element of HSD17B4 suggesting that HSD17B4 plays an important role in aggravated HCC progression. PMID: 25448063
  6. Structural MFE-2 instability is the molecular basis of D-bifunctional protein deficiency type III. PMID: 23308274
  7. Specific combination of compound heterozygous mutations in 17beta-hydroxysteroid dehydrogenase type 4 (HSD17B4) defines a new subtype of D-bifunctional protein deficiency. PMID: 23181892
  8. Molecular models of domain structure of MFE-2 from human, C. elegans, and Drosophila melanogaster lend support to possible structural role of MFE-2 domains including SCP-2L (sterol carrier protein 2-like) domain in human and C. elegans proteins. PMID: 23313254
  9. Epistasis between the HSD17B4 and thyroglobulin polymorphisms is associated with premature ovarian failure. A haplotype in the HSD17B4 gene was identified that was significantly associated with resistance to POF PMID: 22265031
  10. The diagnosis of a type III DBPD with a missense mutation (T15A) in the HSD17B4 gene, coding for D-bifunctional protein (DBP), could be established. PMID: 20949532
  11. MFE2 anchors its substrate around the region from Trp(249) to Arg(251) and positions the substrate along the hydrophobic cavity in the proper direction toward the catalytic center PMID: 20566640
  12. Perrault syndrome and DBP deficiency overlap clinically and Perrault syndrome is genetically heterogeneous. PMID: 20673864
  13. rs11205 in HSD17B4 was associated with testicular germ cell tumor. Risk doubled per copy of the minor A allele. Homozygosity of this allele quadrupled the risk vs. homozygous major G allele. The risk was increased both for seminoma & nonseminoma. PMID: 19776291
  14. crystal structure of 2-enoyl-CoA hydratase 2 PMID: 15644212
  15. Deficiency of this enzyme in man causes a severe developmental syndrome with abnormalities in several organs but in particular in the brain, leading to death within the first year of life. PMID: 16766224
  16. HSD17B4 is not only associated with the presence of prostate cancer, but is also a significant independent predictor of poor patient outcome. PMID: 19100308
  17. HSD17B4 mRNA is expressed in human skin, at similar levels in men and women. HSD17B4 levels are not altered by topical 17-beta-estradiol treatment. PMID: 18794456

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Involvement in disease D-bifunctional protein deficiency (DBPD); Perrault syndrome 1 (PRLTS1)
Subcellular Location Peroxisome
Protein Families Short-chain dehydrogenases/reductases (SDR) family
Tissue Specificity Present in many tissues with highest concentrations in liver, heart, prostate and testis.
Database Links

HGNC: 5213

OMIM: 233400

KEGG: hsa:3295

STRING: 9606.ENSP00000420914

UniGene: Hs.406861

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