Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ATP4A

Uniprot No.

P20648

Research Area

Others

Alternative Names

(Gastric H(+)(+)(Proton pump)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

350-783aa

Target Protein Sequence

TVTVCLSLTAKRLASKNCVVKNLEAVETLGSTSVICSDKTGTLTQNRMTVSHLWFDNHIHTADTTEDQSGQTFDQSSETWRALCRVLTLCNRAAFKSGQDAVPVPKRIVIGDASETALLKFSELTLGNAMGYRDRFPKVCEIPFNSTNKFQLSIHTLEDPRDPRHLLVMKGAPERVLERCSSILIKGQELPLDEQWREAFQTAYLSLGGLGERVLGFCQLYLNEKDYPPGYAFDVEAMNFPSSGLCFAGLVSMIDPPRATVPDAVLKCRTAGIRVIMVTGDHPITAKAIAASVGIISEGSETVEDIAARLRVPVDQVNRKDARACVINGMQLKDMDPSELVEALRTHPEMVFARTSPQQKLVIVESCQRLGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDAAKNAADMILLDDNFASIVTGVEQGRLIFDNL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

54.6 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human ATP4A covers amino acids 350-783. This ATP4A protein is theoretically predicted to have a molecular weight of 54.6 kDa. This ATP4A protein is produced using e.coli expression system. The ATP4A coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant ATP4A protein in following stages of expression and purification.

The human potassium-transporting ATPase alpha chain 1 (ATP4A) is a subunit of the gastric H⁺/K⁺-ATPase, an enzyme crucial for the secretion of gastric acid into the stomach. ATP4A is primarily expressed in the parietal cells of the gastric mucosa and plays a key role in maintaining the acidic environment of the stomach, which is essential for digestion. ATP4A is involved in the active transport of protons (H⁺) into the stomach lumen, where they combine with chloride ions to form hydrochloric acid. This process is critical for the proper breakdown of ingested food. Dysfunction in ATP4A can lead to gastric acid-related disorders, such as peptic ulcers or gastroesophageal reflux disease (GERD). Understanding its function is vital for developing therapeutic strategies targeting acid-related gastrointestinal conditions.

Target Background

Function

The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state.

Gene References into Functions

ATP4A antibodies were present in a significant proportion of children with type 1 diabetes (T1D). Higher ATP4A levels in T1D children are associated with lower, yet still fitting within the normal range, levels of vitamin B12, and ferritin. PMID: 28401620
Heterozygous mutations in the ATP4A and PTH1R genes were identified in a family with type I gastric neuroendocrine tumors plus hypothyroidism and rheumatoid arthritis PMID: 28474257
the generation and characterization of a knockin mouse model for the ATP4a(R703C) mutation to better understand the tumorigenesis process. PMID: 27491072
In conclusion, patients homozygous for an inactivating ATP4A mutation develop gastric ECL-cell carcinoids in their 3rd or 4th decade. PMID: 27150581
Homozygous missense mutation in the 14th exon of the ATP4A gene (c.2107C>T) is responsible for an atypical familial type I gastric neuroendocrine tumour. PMID: 25678551
ATP4A are found in nearly one-third of children with type 1 diabetes and more common among females. In this cross-sectional analysis, Hp infection was not associated with autoimmunity against parietal cells. PMID: 24773566
Downregulation of ATP4A gene is associated with gastric cancer. PMID: 23317218
Gastric H+/K+-ATPase activities are not associated with H pylori status in patients with chronic gastritis. PMID: 15962365
study concludes that IL-1beta upregulates HKalpha transcription by inducing Sp1 binding to HKalpha Sp1 & NF-kappaB sites & that Helicobacter pylori perturbation of HKalpha expression is independent of Sp1-mediated basal HKalpha transcription PMID: 18772363
cigarette smoke extract may cause an increase in the amount and activity of H,K-ATPase and smokers' susceptibility to development of peptic ulcer PMID: 19705497

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Subcellular Location

Apical cell membrane; Multi-pass membrane protein.

Protein Families

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily

Tissue Specificity

Expressed in gastric parietal cells (at protein level).

Database Links

HGNC: 819

OMIM: 137216

KEGG: hsa:495

STRING: 9606.ENSP00000262623

UniGene: Hs.36992

Code	CSB-EP002342HU
Abbreviation	Recombinant Human ATP4A protein, partial
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Potassium-transporting ATPase alpha chain 1 (ATP4A), partial

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