Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
(Gastric H(+)(+)(Proton pump)
Species
Homo sapiens (Human)
Expression Region
350-783aa
Target Protein Sequence
TVTVCLSLTAKRLASKNCVVKNLEAVETLGSTSVICSDKTGTLTQNRMTVSHLWFDNHIHTADTTEDQSGQTFDQSSETWRALCRVLTLCNRAAFKSGQDAVPVPKRIVIGDASETALLKFSELTLGNAMGYRDRFPKVCEIPFNSTNKFQLSIHTLEDPRDPRHLLVMKGAPERVLERCSSILIKGQELPLDEQWREAFQTAYLSLGGLGERVLGFCQLYLNEKDYPPGYAFDVEAMNFPSSGLCFAGLVSMIDPPRATVPDAVLKCRTAGIRVIMVTGDHPITAKAIAASVGIISEGSETVEDIAARLRVPVDQVNRKDARACVINGMQLKDMDPSELVEALRTHPEMVFARTSPQQKLVIVESCQRLGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDAAKNAADMILLDDNFASIVTGVEQGRLIFDNL
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Human ATP4A covers amino acids 350-783. This ATP4A protein is theoretically predicted to have a molecular weight of 54.6 kDa. This ATP4A protein is produced using e.coli expression system. The ATP4A coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant ATP4A protein in following stages of expression and purification.
The human potassium-transporting ATPase alpha chain 1 (ATP4A) is a subunit of the gastric H
+/K
+-ATPase, an enzyme crucial for the secretion of gastric acid into the stomach. ATP4A is primarily expressed in the parietal cells of the gastric mucosa and plays a key role in maintaining the acidic environment of the stomach, which is essential for digestion. ATP4A is involved in the active transport of protons (H
+) into the stomach lumen, where they combine with chloride ions to form hydrochloric acid. This process is critical for the proper breakdown of ingested food. Dysfunction in ATP4A can lead to gastric acid-related disorders, such as peptic ulcers or gastroesophageal reflux disease (GERD). Understanding its function is vital for developing therapeutic strategies targeting acid-related gastrointestinal conditions.
