Recombinant Human Receptor-type tyrosine-protein phosphatase kappa (PTPRK), partial

Code CSB-YP613588HU
MSDS
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Source Yeast
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Code CSB-EP613588HU
MSDS
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Source E.coli
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Code CSB-EP613588HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP613588HU
MSDS
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Source Baculovirus
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Code CSB-MP613588HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PTPRK
Uniprot No.
Alternative Names
dJ480J14.2.1; DKFZp686C2268; DKFZp779N1045; OTTHUMP00000017180; OTTHUMP00000017181; OTTHUMP00000017182; OTTHUMP00000017187; OTTHUMP00000040306; Protein tyrosine phosphatase kappa; Protein tyrosine phosphatase kappa precursor; Protein tyrosine phosphatase receptor type K; Protein tyrosine phosphatase receptor type kappa; Protein-tyrosine phosphatase kappa; PTPK; PTPkappa; Ptprk; PTPRK_HUMAN; R PTP kappa; R-PTP-kappa; Receptor type tyrosine protein phosphatase kappa; Receptor-type tyrosine-protein phosphatase kappa
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Negative regulator of EGFR signaling pathway. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK/PTP-kappa.
Gene References into Functions
  1. PTPRK was identified as a direct target of miR-1260b, and PTPRK expression was inversely correlated with miR-1260b in non-small cell lung cancer cell lines and clinical tissues PMID: 29628123
  2. Study found a significant association between PTPRK genetic variants and the risk and age at onset of Alzheimer's disease in two independent samples, and provided initial evidence of several genetic variants in PTPRK influencing the risk of cancer and cholesterol levels. PMID: 28987514
  3. The present study identified RSPO fusion transcripts, including three novel transcripts, in one-third of colorectal Traditional serrated adenoma (TSA) and showed that PTPRK-RSPO3 fusions were the predominant cause of RSPO overexpression in colorectal TSA. PMID: 28543708
  4. PTPRK plays dual roles in coordinating angiogenesis. It plays a positive role in cell proliferation, adhesion and tubule formation, but suppresses cell migration, in particular, the FGF-promoted migration. PMID: 28259897
  5. PTPRK-RSPO3 fusions and RNF43 mutations were found to be characteristic genetic features of traditional serrated adenomas (TSAs). PMID: 26924569
  6. By regulating phosphorylation of SRC, RPTPkappa promotes the pathogenic action of rheumatoid arthritis fibroblast-like synoviocytes, mediating cross-activation of growth factor and inflammatory cytokine signalling by TGFbeta in RA FLS. PMID: 25378349
  7. Notch and TGF-beta act in concert to stimulate induction of PTPRK, which suppresses EGFR activation in human keratinocytes. PMID: 25609089
  8. Findings strongly indicate that the tyrosine phosphorylation of CD133, which is dephosphorylated by PTPRK, regulates AKT signaling and has a critical role in colon cancer progression. PMID: 24882578
  9. PTPRK underexpression leads to STAT3 activation and contributes to nasal NK/T-cell lymphoma pathogenesis PMID: 25612622
  10. PTPRK showed lower mRNA expression in duodenal mucose of celiac disease patients. PMID: 23820479
  11. High expression of PTPRK is associated with prostate cancer. PMID: 24002526
  12. Tumor derived mutations of protein tyrosine phosphatase receptor type k affect its function and alter sensitivity to chemotherapeutics in glioma. PMID: 23696788
  13. PTPRK is a negative regulator of adhesion, invasion, migration, and proliferation of breast cancer cells. PMID: 23552869
  14. PTPkappa was scissored by the processed form of proprotein convertase 5, and galectin-3 binding protein which is over-produced in colon cancer cells and tissues. PMID: 21094132
  15. These data describe a novel mechanism of cross-talk between EGFR and TGF-beta pathways, in which RPTP-kappa functions to integrate growth-promoting and growth-inhibiting signaling pathways. PMID: 19864455
  16. Our results suggest that GnT-V could decrease human hepatoma SMMC-7721 cell adhesion and promote cell proliferation partially through RPTPkappa. PMID: 19911372
  17. RPTP-kappa is a key regulator of EGFR tyrosine phosphorylation and function in human keratinocytes PMID: 16263724
  18. the crystal structure of catalytically active, monomeric D1 domain of RPTPkappa at 1.9 A. RPTPkappa is monomeric in solution and crystal structure. PMID: 16672235
  19. EGF receptor is activated in human keratinocytes by oxidative inhibition of receptor-type protein-tyrosine phosphatase kappa by ultraviolet irradiation PMID: 16849327
  20. EBNA1 apparently disables TGF-beta signaling, which subsequently decreases transcription of the PTPRK tumor suppressor PMID: 17720884
  21. PTPRK influences transactivating activity of beta-catenin in non-tumoral and neoplastic cells by regulating the balance between signaling and adhesive beta-catenin, thus providing biochemical basis for the hypothesis of PTPRK as a tumor suppressor gene. PMID: 18276111
  22. overexpression of GnT-V in a hepatoma cell line not only induced the addition of beta1,6 GlcNAc branch to N-glycan of RPTPkappa but also decreased the protein level of RPTPkappa PMID: 19236842
  23. These data indicate that PPTRK positively regulates ERK1/2 phosphorylation, which impacts CD4(+) T cell development. PMID: 19800317

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Subcellular Location
Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein-tyrosine phosphatase family, Receptor class 2B subfamily
Tissue Specificity
High levels in lung, brain and colon; less in liver, pancreas, stomach, kidney, placenta and mammary carcinoma.
Database Links

HGNC: 9674

OMIM: 602545

KEGG: hsa:5796

STRING: 9606.ENSP00000357196

UniGene: Hs.155919

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