Recombinant Human SPRY domain-containing SOCS box protein 1 (SPSB1) is produced in E. coli and includes the complete sequence spanning 1-273 amino acids. The protein comes with an N-terminal 10xHis-tag and a C-terminal Myc-tag, which makes purification and detection more straightforward. Purity levels appear to exceed 85%, based on SDS-PAGE analysis. This product is designed strictly for research purposes and may provide consistent results in experimental settings.
SPSB1 belongs to the suppressor of cytokine signaling (SOCS) box protein family. This protein family is recognized for its role in regulating different signaling pathways. SPSB1 seems to interact with components of the ubiquitin-proteasome system, potentially affecting how proteins get degraded. Research suggests SPSB1 plays an important role in cellular signaling, which could make it a valuable tool for studying protein regulation and signaling dynamics.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies Using Pull-Down Assays
This dual-tagged SPSB1 protein offers useful options for exploring protein-protein interactions through pull-down experiments. The His-tag allows researchers to immobilize the protein on nickel-affinity resins. Meanwhile, the Myc-tag helps with detection and confirms that protein capture has occurred. Scientists can use this full-length recombinant protein (1-273aa) to hunt for new binding partners or verify known interactions with other SOCS box family members or E3 ligase components. The 85% purity level appears adequate for interaction studies where the protein acts as bait in biochemical assays.
2. Antibody Development and Validation
Researchers may find this recombinant SPSB1 protein useful as an antigen for creating specific antibodies against human SPSB1. It might also work well for validating antibodies that already exist. Since it's the full-length protein, it offers multiple epitopes for antibody recognition. This characteristic likely makes it suitable for both polyclonal and monoclonal antibody production. The dual-tag system appears to simplify purification and quality control during antibody screening. Scientists can probably use this protein in ELISA-based assays to assess antibody specificity and binding strength.
3. Biochemical Characterization and Stability Studies
This recombinant SPSB1 protein could work well for detailed biochemical characterization. Such studies might include thermal stability analysis, pH tolerance testing, and buffer optimization experiments. Researchers can track the protein's behavior under different conditions by using the Myc-tag for detection in Western blot analyses. These kinds of studies would likely reveal fundamental information about SPSB1 protein properties. They might also help establish the best storage and handling conditions for future research work.
4. In Vitro Binding Assays with Tagged Detection
The C-terminal Myc-tag makes it possible to develop sandwich-type binding assays. In these assays, SPSB1 interactions can be detected using anti-Myc antibodies. This method seems particularly helpful for studying dose-dependent binding relationships and competitive binding experiments. At the same time, the His-tag allows for protein immobilization. Together, these features create what appears to be a flexible experimental system for quantitative interaction studies that rely on colorimetric or fluorescent detection methods.
