Recombinant Human SMARCB1 is produced in a yeast expression system and covers amino acids 2 to 376 of the protein sequence. An N-terminal 6xHis-tag has been added to help with purification and detection. SDS-PAGE analysis confirms the protein shows greater than 90% purity. This product is for research use only and appears to contain no detectable endotoxin levels.
SMARCB1 acts as a core component of the SWI/SNF chromatin remodeling complex and seems to play a crucial role in controlling gene expression by changing chromatin structure. The protein is involved in several cellular processes, including cell cycle control and differentiation. Because of its central position in chromatin remodeling, SMARCB1 has become a major focus for researchers studying gene regulation mechanisms and epigenetic modifications.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies
This recombinant SMARCB1 protein can help investigate how it interacts with other SWI/SNF complex components and chromatin remodeling factors through pull-down assays using the N-terminal 6xHis tag. The partial protein covering amino acids 2-376 retains key functional domains that may be involved in protein-protein interactions within the chromatin remodeling machinery. Co-immunoprecipitation experiments and GST pull-down assays could help map binding partners. These approaches might also characterize the molecular mechanisms behind SWI/SNF complex assembly.
2. Antibody Development and Validation
The high purity recombinant SMARCB1 protein works well as an antigen for generating specific antibodies against human SMARCB1. The 6xHis tag makes purification and immobilization easier during immunization protocols and subsequent antibody screening assays. Researchers can also use this protein to validate the specificity of existing SMARCB1 antibodies. Western blot, ELISA, and other immunoassays help ensure proper recognition of the target protein.
3. Structural and Biophysical Characterization
The purified recombinant protein provides material for structural studies. X-ray crystallography, NMR spectroscopy, and cryo-electron microscopy may help reveal SMARCB1's molecular architecture. Various biophysical techniques can characterize the protein's properties - dynamic light scattering, analytical ultracentrifugation, and thermal stability assays appear useful for examining folding state, oligomerization properties, and stability under different conditions. These studies likely contribute to understanding the structural basis of SMARCB1 function in chromatin remodeling complexes.
4. In Vitro Chromatin Remodeling Assays
Researchers can incorporate this recombinant SMARCB1 into reconstituted SWI/SNF complexes for in vitro chromatin remodeling studies using nucleosome substrates. The protein helps investigate its role in nucleosome sliding, histone octamer transfer, and chromatin accessibility changes. Gel mobility shift assays and nuclease accessibility experiments provide ways to measure these effects. Such studies may help clarify the mechanistic contribution of SMARCB1 to the overall chromatin remodeling activity of SWI/SNF complexes.
5. Biochemical Assay Development
The 6xHis-tagged SMARCB1 protein can work as a standard or control in various biochemical assays designed to study chromatin remodeling mechanisms. Researchers might use it to develop and optimize assays measuring protein stability, post-translational modifications, or enzymatic activities of associated factors. The consistent quality and purity of this recombinant protein makes it suitable for quantitative biochemical studies. It also appears useful for high-throughput screening applications focused on chromatin biology research.
