Recombinant Human Speedy protein C (SPDYC)

Code	CSB-YP702803HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP702803HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP702803HU-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP702803HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP702803HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

SPDYC

Uniprot No.

Q5MJ68

Alternative Names

SPDYC; Speedy protein C; Rapid inducer of G2/M progression in oocytes C; RINGO C; hSpy/Ringo C

Species

Homo sapiens (Human)

Expression Region

1-293

Target Protein Sequence

MLWAIPELGS PCPISISYEM SDSQDPTTSP VVTTQVELGG CSRQGGGNGF LRFRQHQEVQ AFLSLLEDSF VQEFLSKDPC FQISDKYLLA MVLVYFQRAH LKLSEYTHSS LFLALYLAND MEEDLEGPKC EIFPWALGKD WCLRVGKFLH QRDKLWARMG FRAVVSRQCC EEVMAKEPFH WAWTRDRRPH HGGVQRVCPQ VPVRLPRGPG LSPPHCSPCG LPQHCSSHLL KPVSSKCPSL TSECHRPPSQ NYLSRVKNAW GGDFLIVLPP QMQLEPGTYS LRIFPKPPAR PGH

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Promotes progression through the cell cycle via binding and activation of CDK1 and CDK2. Involved in the spindle-assembly checkpoint. Required for recruitment of MAD2L1, BUBR1 and BUB1 to kinetochores. Required for the correct localization of the active form of Aurora B in prometaphase.

Gene References into Functions

Results indicate a role for RINGO C in the mitotic checkpoint, which might be mediated by defective recruitment of SAC components and deregulation of the activity of Aurora kinase B. PMID: 20605920
CDK-Speedy/Ringo C complexes positively regulate cell cycle progression during the late S and G(2) phases of the cell cycle. PMID: 18802405

Subcellular Location

Cytoplasm. Note=Colocalizes with tubulin gamma during interphase. During mitosis, enriched at the spindle poles and the midbody during telophase.

Protein Families

Speedy/Ringo family

Tissue Specificity

Expressed in a variety of tissues including bone marrow, kidney, small intestine, liver, placenta and testis.

Database Links

HGNC: 32681

OMIM: 614030

KEGG: hsa:387778

STRING: 9606.ENSP00000366390

UniGene: Hs.450642