Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

STX11

Uniprot No.

O75558

Research Area

Tags & Cell Markers

Alternative Names

FHL4; HLH4; HPLH4 ; Stx11; STX11_HUMAN; Syntaxin 11 ; Syntaxin-11

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-287aa

Target Protein Sequence

MKDRLAELLDLSKQYDQQFPDGDDEFDSPHEDIVFETDHILESLYRDIRDIQDENQLLVADVKRLGKQNARFLTSMRRLSSIKRDTNSIAKAIKARGEVIHCKLRAMKELSEAAEAQHGPHSAVARISRAQYNALTLTFQRAMHDYNQAEMKQRDNCKIRIQRQLEIMGKEVSGDQIEDMFEQGKWDVFSENLLADVKGARAALNEIESRHRELLRLESRIRDVHELFLQMAVLVEKQADTLNVIELNVQKTVDYTGQAKAQVRKAVQYEEKNPCRTLCCFCCPCLK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

60.2kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human STX11 contains amino acids 1-287. The expected molecular weight for the STX11 protein is calculated to be 60.2 kDa. This protein is generated in a e.coli-based system. The STX11 coding gene included the N-terminal GST tag, which simplifies the detection and purification processes of the recombinant STX11 protein in following stages of expression and purification.

Syntaxin-11 (STX11) is a member of the syntaxin family of proteins, which are involved in vesicle trafficking and membrane fusion events within cells. STX11 is specifically implicated in regulating the fusion of vesicles with lysosomes, playing a crucial role in the process of lysosomal exocytosis. This protein is predominantly expressed in hematopoietic cells, including NK cells and CTLs, where it is involved in the release of cytotoxic granules containing perforin and granzymes. The interaction between STX11 and Munc18-2, another protein involved in vesicle trafficking, is essential for proper vesicle fusion and cytotoxic granule release. Mutations in the STX11 gene are associated with familial hemophagocytic lymphohistiocytosis type 4 (FHL4), a rare and severe immune disorder characterized by uncontrolled activation of immune cells. Understanding the functions of STX11 is crucial for unraveling the molecular mechanisms underlying vesicle trafficking and immune responses in specific cell types.

Target Background

Function

SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network.

Gene References into Functions

Neonatal platelets exhibit low levels of the Stx11-Munc18b complex (essential component of the SNARE machinery) and of beta1-tubulin. These developmental deficiencies are associated with defects in platelet adhesion, spreading and secretion. PMID: 29044293
The results suggest that STX11 plays an important role in the pathogenesis of Peripheral T-cell lymphomas and they may contribute to the future development of new drugs for the treatment of Peripheral T-cell lymphomas. PMID: 26176172
a pivotal role for S-acylation in the function of syntaxin 11 in NK cells PMID: 24910990
Stx11 functions as a t-SNARE for the final fusion of LG at the IS. PMID: 24227526
Distinct severity of HLH in both human and murine mutants with complete loss of cytotoxic effector PRF1, RAB27A, and STX11. PMID: 23160464
These data indicate that human neutrophils express syntaxin 11 and call attention to the possible involvement of neutrophils in familial hemophagocytic lymphohistiocytosis pathology PMID: 19259622
Platelets deficient in syntaxin-11 from a Familial Hemophagocytic Lymphohistiocytosis type 4 had secretion defect. PMID: 22767500
Data suggest that syntaxin 11 promotes the fusion of Rab27a-expressing vesicles with cytotoxic granules and reveal additional complexity in spatial/temporal segregation of subcellular structures involved in granule-mediated cytotoxicity. PMID: 21342435
No detrimental mutations were identified in STX11 in Chinese children with Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis. PMID: 21674762
STX11 should be sequenced in HLH patients even when impaired NK cell degranulation is not found PMID: 21298754
a novel homozygous deletion (c. 581_584delTGCC; p.Leu194ProfsX2) in the gene-encoding syntaxin 11 (STX11), causing a premature termination codon in hemophagocytic lymphohistiocytosis PMID: 19967551
The mutations in STX11 are responsible for HLH in approximately 1% of North American patients and can cause variable defects in syntaxin 11 expression and function with resultant impact on clinical phenotype. PMID: 20486178
A large group of 63 unrelated patients with Familial hemophagocytic lymphohistiocytosis (FHL) was analysed for mutations in STX11, PRF1, and UNC13D. PMID: 16278825
Defective cytotoxic lymphocyte degranulation is associated with syntaxin-11 deficient familial hemophagocytic lymphohistiocytosis 4 patients PMID: 17525286
Syntaxin 11 plays a role in natural killer (NK) cell granule exocytosis and in the generation of cell-mediated killing. PMID: 17785771
DNA methylation of Stx11 contribute to disease susceptibility at the 6q24 locus in humans. PMID: 19169743
Familial hemophagocytic lymphohistiocytosis type 5 (FHL-5) is caused by mutations in Munc18-2 and impaired binding to syntaxin 11 PMID: 19804848

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Involvement in disease

Familial hemophagocytic lymphohistiocytosis 4 (FHL4)

Subcellular Location

Membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein.

Protein Families

Syntaxin family

Database Links

HGNC: 11429

OMIM: 603552

KEGG: hsa:8676

STRING: 9606.ENSP00000356540

UniGene: Hs.118958

Code	CSB-EP022889HU
Abbreviation	Recombinant Human STX11 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Syntaxin-11 (STX11)

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