Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Tags & Cell Markers
Alternative Names
FHL4; HLH4; HPLH4 ; Stx11; STX11_HUMAN; Syntaxin 11 ; Syntaxin-11
Species
Homo sapiens (Human)
Expression Region
1-287aa
Target Protein Sequence
MKDRLAELLDLSKQYDQQFPDGDDEFDSPHEDIVFETDHILESLYRDIRDIQDENQLLVADVKRLGKQNARFLTSMRRLSSIKRDTNSIAKAIKARGEVIHCKLRAMKELSEAAEAQHGPHSAVARISRAQYNALTLTFQRAMHDYNQAEMKQRDNCKIRIQRQLEIMGKEVSGDQIEDMFEQGKWDVFSENLLADVKGARAALNEIESRHRELLRLESRIRDVHELFLQMAVLVEKQADTLNVIELNVQKTVDYTGQAKAQVRKAVQYEEKNPCRTLCCFCCPCLK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Human STX11 contains amino acids 1-287. The expected molecular weight for the STX11 protein is calculated to be 60.2 kDa. This protein is generated in a e.coli-based system. The STX11 coding gene included the N-terminal GST tag, which simplifies the detection and purification processes of the recombinant STX11 protein in following stages of expression and purification.
Syntaxin-11 (STX11) is a member of the syntaxin family of proteins, which are involved in vesicle trafficking and membrane fusion events within cells. STX11 is specifically implicated in regulating the fusion of vesicles with lysosomes, playing a crucial role in the process of lysosomal exocytosis. This protein is predominantly expressed in hematopoietic cells, including NK cells and CTLs, where it is involved in the release of cytotoxic granules containing perforin and granzymes. The interaction between STX11 and Munc18-2, another protein involved in vesicle trafficking, is essential for proper vesicle fusion and cytotoxic granule release. Mutations in the STX11 gene are associated with familial hemophagocytic lymphohistiocytosis type 4 (FHL4), a rare and severe immune disorder characterized by uncontrolled activation of immune cells. Understanding the functions of STX11 is crucial for unraveling the molecular mechanisms underlying vesicle trafficking and immune responses in specific cell types.
