This UMOD protein is a semi-custom product. There are 5 expression system options: Yeast, E. coli, In Vivo Biotinylation in E. coli, Baculovirus, and Mammalian cell. Your requirements will be given top priority in determining the protein tags. For proteins within 800 aa, risk-free custom service is guaranteed. It means you will not be charged if the protein cannot be delivered.
UMOD, also known as Tamm-Horsfall protein, is a heavily glycosylated protein with a complex tertiary structure due to the fairly high number of disulfide bonds [1]. It is the most abundant protein in human urine and is exclusively produced by the kidney tubule [2][3]. UMOD has been recognized as an important regulatory protein of the innate immune system via binding complement fragments [4]. Additionally, UMOD plays a crucial role in protecting against urinary tract infections and renal calcium crystal formation, and it is a promoter of hypertension [5]. The UMOD protein contains a zona pellucida domain (ZP), an elastase-sensitive fragment containing three calcium-binding epidermal growth factor (EGF)-like domains and a signal peptide, and a potential glycosyl-phosphatidylinositol (GPI)-anchor cleavage site [6]. Furthermore, UMOD regulates renal magnesium homeostasis through the ion channel transient receptor potential melastatin 6 (TRPM6) [5]. Mutations in the UMOD gene can lead to kidney diseases such as autosomal dominant tubulointerstitial kidney disease (ADTKD-UMOD) [7]. UMOD has also been associated with acute kidney injury in patients undergoing cardiac surgery [8].
References:
[1] S. Mary, P. Boder, G. Rossitto, L. Graham, K. Scott, A. Flynnet al., Salt loading decreases urinary excretion and increases intracellular accumulation of uromodulin in stroke-prone spontaneously hypertensive rats, Clinical Science, vol. 135, no. 24, p. 2749-2761, 2021. https://doi.org/10.1042/cs20211017
[2] O. Devuyst and C. Pattaro, The umod locus: insights into the pathogenesis and prognosis of kidney disease, Journal of the American Society of Nephrology, vol. 29, no. 3, p. 713-726, 2017. https://doi.org/10.1681/asn.2017070716
[3] A. Köttgen, N. Glazer, A. Dehghan, S. Hwang, R. Katz, M. Liet al., Multiple loci associated with indices of renal function and chronic kidney disease, Nature Genetics, vol. 41, no. 6, p. 712-717, 2009. https://doi.org/10.1038/ng.377
[4] L. Bai, Q. Xie, M. Xia, K. Gong, N. Wang, Y. Chenet al., The importance of sialic acid, ph and ion concentration on the interaction of uromodulin and complement factor h, Journal of Cellular and Molecular Medicine, vol. 25, no. 9, p. 4316-4325, 2021. https://doi.org/10.1111/jcmm.16492
[5] M. Nie, M. Bal, J. Liu, Z. Yang, C. Rivera, X. Wuet al., Uromodulin regulates renal magnesium homeostasis through the ion channel transient receptor potential melastatin 6 (trpm6), Journal of Biological Chemistry, vol. 293, no. 42, p. 16488-16502, 2018. https://doi.org/10.1074/jbc.ra118.003950
[6] M. Wolf, B. Mucha, M. Attanasio, I. Zalewski, S. Karle, H. Neumannet al., Mutations of the uromodulin gene in mckd type 2 patients cluster in exon 4, which encodes three egf-like domains, Kidney International, vol. 64, no. 5, p. 1580-1587, 2003. https://doi.org/10.1046/j.1523-1755.2003.00269.x
[7] G. Schiano, J. Lake, M. Mariniello, C. Schaeffer, M. Harvent, L. Rampoldiet al., Allelic and gene dosage effects involving uromodulin aggregates drive autosomal dominant tubulointerstitial kidney disease,, 2022. https://doi.org/10.1101/2022.09.13.507770
[8] D. Chojęta, M. Kozioł, S. Targońska, & I. Smarz-Widelska, Association of uromodulin with acute kidney injury in patients undergoing cardiac surgery, Polish Journal of Cardio-Thoracic Surgery, vol. 17, no. 3, p. 160-164, 2020. https://doi.org/10.5114/kitp.2020.99081
