Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

VSTM5

Uniprot No.

A8MXK1

Research Area

Cell Biology

Alternative Names

VSTM5; C11orf90; V-set and transmembrane domain-containing protein 5

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

29-147aa

Target Protein Sequence

LQSQGVSLYIPQATINATVKEDILLSVEYSCHGVPTIEWTYSSNWGTQKIVEWKPGTQANISQSHKDRVCTFDNGSIQLFSVGVRDSGYYVITVTERLGSSQFGTIVLHVSEILYEDLH
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

19.3 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human VSTM5 contains amino acids 29-147. This VSTM5 protein is theoretically predicted to have a molecular weight of 19.3 kDa. The VSTM5 protein was expressed in e.coli. The VSTM5 gene fragment has been modified by fusing the N-terminal 10xHis tag, providing convenience in detecting and purifying the recombinant VSTM5 protein during the following stages.

V-set and transmembrane domain-containing protein 5 (VSTM5) is a cell-adhesion-like molecule belonging to the immunoglobulin (Ig) superfamily. Studies have found that VSTM5 is an N-glycosylated membrane protein highly expressed in the central nervous system (CNS). It promoted the development of neuronal dendrites and protrusions, such as dendritic filopodia. During synaptogenesis, VSTM5 modulates synapse formation by modifying dendritic spine morphology and the distribution of actin. VSTM5 plays a role in regulating neuronal morphogenesis and migration during cortical development in the mouse brain. These findings suggest that VSTM5 could be a new membrane protein directly modulating membrane dynamics for neuronal morphogenesis and migration, potentially influencing neural circuits and brain functions.

Target Background

Function

Cell adhesion-like membrane protein of the central nervous system (CNS) which modulates both the position and complexity of central neurons by altering their membrane morphology and dynamics. Involved in the formation of neuronal dendrites and protrusions including dendritic filopodia. In synaptogenesis, regulates synapse formation by altering dendritic spine morphology and actin distribution. Promotes formation of unstable neuronal spines such as thin and branched types. Regulates neuronal morphogenesis and migration during cortical development in the brain.

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection, dendrite. Cell projection, axon.

Database Links

HGNC: 34443

KEGG: hsa:387804

STRING: 9606.ENSP00000386607

UniGene: Hs.512252

Code	CSB-EP003050HU1
Abbreviation	Recombinant Human VSTM5 protein, partial
MSDS	MSDS Datasheet
Size	$388
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human V-set and transmembrane domain-containing protein 5 (VSTM5), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage