Recombinant Mouse ADAM DEC1 (Adamdec1) is expressed in E. coli and contains the full length of the mature protein, spanning amino acids 209 to 467. The protein comes with an N-terminal 6xHis-tag that helps with purification and detection. SDS-PAGE analysis confirms the product's purity exceeds 90%, which appears to make it suitable for various research applications.
ADAM DEC1 belongs to the ADAM (a disintegrin and metalloprotease) protein family. These proteins seem to be involved in several cellular processes, including cell signaling and adhesion. This particular protein likely plays an important role in regulating extracellular matrix composition and may be significant in studies related to tissue remodeling and repair. Understanding how it works could be crucial for advancing research in cell biology and related fields.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies Using His-Tag Pull-Down Assays
The N-terminal 6xHis-tag on this recombinant mouse ADAM DEC1 protein allows for nickel-affinity purification and pull-down experiments to identify potential binding partners. Researchers can attach the His-tagged protein to nickel-coated beads or columns, then incubate with cell lysates or purified protein libraries to capture interacting molecules. Mass spectrometry or Western blotting can then analyze the captured complexes to identify novel ADAM DEC1 interaction partners. This approach may be particularly valuable for studying the molecular mechanisms that underlie ADAM DEC1 function in cellular processes.
2. Antibody Development and Validation
The high purity (>90%) and defined expression region (209-467aa) suggest this recombinant protein could work well as an immunogen for generating specific antibodies against mouse ADAM DEC1. The protein can immunize rabbits or mice for polyclonal antibody production, or serve as an antigen for monoclonal antibody screening. The purified protein also acts as a positive control for validating antibody specificity in Western blotting, ELISA, and immunoprecipitation experiments targeting endogenous ADAM DEC1 in mouse tissues or cell lines.
3. Biochemical Characterization and Protein Stability Studies
This recombinant ADAM DEC1 protein can be used for comprehensive biochemical analysis. This includes determining optimal storage conditions, pH stability, thermal stability, and buffer compatibility. Dynamic light scattering, differential scanning fluorimetry, and size exclusion chromatography can help characterize the protein's biophysical properties. These studies provide essential information for optimizing experimental conditions and understanding how the protein behaves in various research applications.
4. ELISA-Based Quantitative Assays
The His-tagged recombinant protein can work as a standard or capture antigen in enzyme-linked immunosorbent assays (ELISA) for quantifying ADAM DEC1 levels in biological samples. The protein can be attached to ELISA plates either directly or through anti-His antibodies, which enables development of sandwich or competitive ELISA formats. This application appears particularly useful for studying ADAM DEC1 expression patterns in different mouse tissues, cell types, or experimental conditions in research settings.
