Recombinant Mouse ADP-ribosylation factor 1(Arf1)

Code CSB-YP001988MO
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Source Yeast
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Code CSB-EP001988MO
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Source E.coli
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Code CSB-EP001988MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001988MO
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Source Baculovirus
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Code CSB-MP001988MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Arf1
Uniprot No. P84078
Alternative Names Arf1; ADP-ribosylation factor 1
Species Mus musculus (Mouse)
Expression Region 2-181
Target Protein Sequence GNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
GTP-binding protein involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Gene References into Functions
  1. The authors found that Arf1 occupies contrasting molecular environments within the Coat Protein Complex I, leading them to hypothesize that some Arf1 molecules may regulate vesicle assembly while others regulate coat disassembly. PMID: 28621666
  2. ARF1 may be a plausible inter-player that mediates the transition to osteoclast fusion at multiple steps during osteoclast differentiation. PMID: 26690137
  3. Increased gene dosage of Ink4a, Arf1 and p53 delays age-associated central nervous system functional decline. PMID: 25990896
  4. ARF1/TBCE-mediated cross-talk that coordinates COPI formation and tubulin polymerization at the Golgi. PMID: 24951541
  5. These data establish for the first time that the Arf1 gene is indispensable for mouse embryonic development after implantation. PMID: 25305484
  6. Arf modulates LRP6 phosphorylation for the transduction of Wnt/beta-catenin signaling. PMID: 22907437
  7. Down-regulation of ADP-ribosylation factor results in corneal neovascularization regression. PMID: 23288987
  8. ARF1-dependent trafficking of procathepsin B and the maturation of autophagosomes results in cathepsin B-mediated trypsinogen activation induced by caerulein. PMID: 22570480
  9. Egr1 mediates p53-independent c-Myc-induced apoptosis via a noncanonical ARF-dependent transcriptional mechanism PMID: 21187408
  10. A senescence rescue screen identifies BCL6 as an inhibitor of anti-proliferative p19(ARF)-p53 signaling PMID: 11914273
  11. termination of Arf1 signals mediated through GGA require that Arf1.GTP dissociates from GGA prior to interaction with GAP and consequent hydrolysis of GTP PMID: 12376537
  12. GDP-bound ARF1 induces dissociation of ADRP from the Lipid droplet surface. PMID: 15336557
  13. The recruitment of certain adhesion components such as paxillin requires dynamic GTP/GDP turnover of Arf1 GTPase. PMID: 15632162
  14. The crystal structure of the activated GTP-bound form of ARF1 in a complex with the Arf-binding domain (ArfBD) of ARHGAP21 at 2.1 A resolution, is presented. PMID: 17347647
  15. ARF1 plays an essential role in regulating asymmetric cell division in female meiosis. PMID: 19005166

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Subcellular Location Golgi apparatus. Cytoplasm, perinuclear region. Cell junction, synapse, synaptosome. Cell junction, synapse, postsynaptic density. Membrane; Lipid-anchor. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor.
Protein Families Small GTPase superfamily, Arf family
Database Links

KEGG: mmu:11840

STRING: 10090.ENSMUSP00000079905

UniGene: Mm.371546

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