Recombinant Mouse Adipocyte enhancer-binding protein 1 (Aebp1), partial

Very nice to receive your inquiry. Regarding your request for "endotoxin-removal, lyophilized, glycerol content (50%), concentration requirement (> 0.6 mg/mL). "
I guess you need the liquid protein not lyophilized, right? Since you indicate the glycerol content and concentration, which is only suitable for liquid protein.
We can meet all your requirements, please remark your requirements when placing the order
CSB-EP723715MO >> E.coli 3-7 working days (In Stock)
Generally, we will not add any glycerol in the buffer prior to lyophilization if you need the lyophilized protein, because glycerol is not good for lyophilization, even if the buffer contains glycerol, we will try to remove glycerol by dialysis before lyophilization.
The concentration is based on liquid protein, you can dissolve the lyophilized protein and adjust the concentration according to your own demand.
Reconstitution
(1)The reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins. (Dissolve completely. The concentration could depend on your experiment demand.)
(2)It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration.
If you have special requirements for concentration, then you could dilute the protein according to your experiment demand.
(3)We haven’t studied whether the optimal concentration range will vary among different hosts such as E.coli, yeast, mammalian or baculovirus, so we can’t provide useful advice.
(4)If you will use immediately after dissolving one protein, then you don't need to add glycerol. If you won't use the protein in short term, we suggest to add some glycerol for storage.
We suggest you to reconstitute suitable amount of protein and store the remaining protein in lyophilized powder. You could remark "The lyophilized powder should be packed into multiple vials" when placing order.
The amount of lyophilized powder in each vial will depend on your experiment demand.
(5)In principle, the final concentration of glycerol ranges from 5% to 50% will be OK. Our default final concentration of glycerol is 50%. You could use it as reference.