Recombinant Mouse Adipocyte enhancer-binding protein 1(Aebp1) ,partial

In Stock
Code CSB-EP723715MO
Size US$2466Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP723715MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Aebp1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP723715MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Aebp1.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Aebp1
Uniprot No. Q640N1
Research Area Epigenetics and Nuclear Signaling
Alternative Names Aebp1; Aclp; Adipocyte enhancer-binding protein 1; AE-binding protein 1; Aortic carboxypeptidase-like protein
Species Mus musculus (Mouse)
Source E.coli
Expression Region 26-370aa
Target Protein Sequence QTVLTDDEIEEFLEGFLSELETQSPPREDDVEVQPLPEPTQRPRKSKAGGKQRADVEVPPEKNKDKEKKGKKDKGPKATKPLEGSTRPTKKPKEKPPKATKKPKEKPPKATKKPKEKPPKATKKPKEKPPKATKRPSAGKKFSTVAPLETLDRLLPSPSNPSAQELPQKRDTPFPNAWQGQGEETQVEAKQPRPEPEEETEMPTLDYNDQIEKEDYEDFEYIRRQKQPRPTPSRRRLWPERPEEKTEEPEERKEVEPPLKPLLPPDYGDSYVIPNYDDLDYYFPHPPPQKPDVGQEVDEEKEEMKKPKKEGSSPKEDTEDKWTVEKNKDHKGPRKGEELEEEWAP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 55.8kDa
Protein Length Partial
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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 Q&A
Q:

We want the protein “endotoxin-removal, lyophilized, glycerol content (50%), concentration requirement (> 0.6 mg/mL).
Is it typical to have glycerol in the buffer prior to lyophilization, and can’t the customer just make their own concentration when its lyophilized?

A:
Very nice to receive your inquiry. Regarding your request for "endotoxin-removal, lyophilized, glycerol content (50%), concentration requirement (> 0.6 mg/mL). "
I guess you need the liquid protein not lyophilized, right? Since you indicate the glycerol content and concentration, which is only suitable for liquid protein.
We can meet all your requirements, please remark your requirements when placing the order
CSB-EP723715MO >> E.coli 3-7 working days (In Stock)
Generally, we will not add any glycerol in the buffer prior to lyophilization if you need the lyophilized protein, because glycerol is not good for lyophilization, even if the buffer contains glycerol, we will try to remove glycerol by dialysis before lyophilization.
The concentration is based on liquid protein, you can dissolve the lyophilized protein and adjust the concentration according to your own demand.
Reconstitution
(1)The reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins. (Dissolve completely. The concentration could depend on your experiment demand.)
(2)It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration.
If you have special requirements for concentration, then you could dilute the protein according to your experiment demand.
(3)We haven’t studied whether the optimal concentration range will vary among different hosts such as E.coli, yeast, mammalian or baculovirus, so we can’t provide useful advice.
(4)If you will use immediately after dissolving one protein, then you don't need to add glycerol. If you won't use the protein in short term, we suggest to add some glycerol for storage.
We suggest you to reconstitute suitable amount of protein and store the remaining protein in lyophilized powder. You could remark "The lyophilized powder should be packed into multiple vials" when placing order.
The amount of lyophilized powder in each vial will depend on your experiment demand.
(5)In principle, the final concentration of glycerol ranges from 5% to 50% will be OK. Our default final concentration of glycerol is 50%. You could use it as reference.

Target Data

Function Isoform 2 may positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. Isoform 2 may also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I-kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor.
Gene References into Functions
  1. ACLP stimulates the fibroblast-to-myofibroblast transition by promoting SMA expression via TGFbeta signaling and promoting collagen expression through a TGFbeta receptor-independent pathway. PMID: 24344132
  2. Stromal adipocyte enhancer-binding protein (AEBP1) promotes mammary epithelial cell hyperplasia via proinflammatory and hedgehog signaling PMID: 22995915
  3. Data suggest that macrophage AEBP1 plays critical regulatory roles in atherogenesis, and it may serve as a potential therapeutic target for the prevention or treatment of atherosclerosis. PMID: 21687917
  4. Results describe the pathophysiological features of gastroschisis in aortic carboxypeptidase-like protein knockout mice and to characterize the neuromuscular development of the eviscerated intestine. PMID: 20386491
  5. MAPK regulates the transcriptional activity of AEBP1 by a novel dual mechanism, in which MAPK interaction enhances and subsequent phosphorylation decreases the DNA-binding ability of AEBP1 PMID: 15654748
  6. Developmental expression pattern is consistent with a regulatory or structural role in the abdominal wall, vasculature, and dermis. PMID: 15749083
  7. AEBP1 plays critical regulatory roles in macrophage cholesterol homeostasis, foam cell formation, and proinflammation. PMID: 16461908
  8. NF-kappaB activity is modulated by means of protein-protein interaction involving AEBP1 and IkappaBalpha. PMID: 17202411
  9. AEBP1 negatively regulates adipose tissue PTEN levels and has a key functional role in modulation of in vivo adiposity. PMID: 17299101
  10. Adipocyte enhancer-binding protein 1 (AEBP1) is up-regulated during monocyte differentiation. PMID: 19166963
  11. ACLP and its discoidin-like domain may be novel targets for anti-myofibroblast-based therapies for the treatment of pulmonary fibrosis. PMID: 19179605

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Subcellular Location Isoform 1: Secreted, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm, Nucleus
Protein Families Peptidase M14 family
Tissue Specificity Isoform 1 and isoform 2 are expressed in adipose tissue, brain, heart, kidney, liver, lung, skeletal muscle, small intestine, spleen and testis. Isoform 2 is expressed in macrophages. Expressed in aorta, preadipocytes, adipocyte tissue, brain, heart, live
Database Links

KEGG: mmu:11568

STRING: 10090.ENSMUSP00000099987

UniGene: Mm.271178

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