Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Slc7a10

Uniprot No.

P63115

Research Area

others

Alternative Names

Slc7a10; Asc1; Asc-type amino acid transporter 1; Asc-1; D-serine transporter; Solute carrier family 7 member 10

Species

Mus musculus (Mouse)

Source

Yeast

Mol. Weight

22.5kDa

Protein Length

Partial

Tag Info

N-terminal His-tagged
Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Mouse Slc7a10 was expressed with the amino acid range of 475-530. This Slc7a10 protein is expected to have a theoretical molecular weight of 22.5 kDa. This Slc7a10 protein is produced using yeast expression system. The Slc7a10 gene fragment has been modified by fusing the N-terminal 6xHis-sumostar tag, providing convenience in detecting and purifying the recombinant Slc7a10 protein during the following stages.

The mouse asc-type amino acid transporter 1 (Slc7a10) is a sodium-independent amino acid transporter that facilitates the uptake of small, neutral amino acids into cells. Slc7a10 is prominently expressed in the brain, particularly in regions like the hippocampus and cortex. The Slc7a10's presence in these areas suggests its role in regulating amino acid levels crucial for neurotransmission and neuronal function. Studies have linked alterations in Slc7a10 expression to neurological disorders. It may play a role in maintaining amino acid homeostasis in the central nervous system. Understanding the function of mouse Slc7a10 is essential for unraveling its role in amino acid metabolism, neurotransmission, and potential contributions to neurological disorders in mice.

Target Background

Function

Sodium-independent, high affinity transport of small neutral D- and L-amino acids and amino acid-related compounds. May play a role in the modulation of glutamatergic transmission through mobilization of D-serine at the glutamatergic synapse.

Gene References into Functions

this study shows that the astrocytic transporter SLC7A10 mediates glycinergic inhibition of spinal cord motor neurons PMID: 27759100
Asc-1 has a novel role in regulating NMDA receptor-dependent synaptic activity by mediating concurrent non-vesicular release of D-serine and glycine. PMID: 23426681
The alanine-serine-cysteine-1 (Asc-1) transporter controls glycine levels in the brain and is required for glycinergic inhibitory transmission PMID: 25755256
The amino acid transporter ASC-1 is a white adipocyte-specific cell surface protein. PMID: 25080478
Lack of the alanine-serine-cysteine transporter 1 causes tremors, seizures, and early postnatal death in mice. PMID: 16026768
The results presented reveal that Asc-1 is the only high affinity D-serine transporter in the mouse CNS and is the predominant mechanism for D-serine reuptake. PMID: 17432963

Hide All

Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

Amino acid-polyamine-organocation (APC) superfamily

Tissue Specificity

Highly expressed in brain and lung, and to a lesser extent in placenta and small intestine.

Database Links

KEGG: mmu:53896

STRING: 10090.ENSMUSP00000001854

UniGene: Mm.35567

Code	CSB-YP021710MO
Abbreviation	Recombinant Mouse Slc7a10 protein, partial
MSDS	MSDS Datasheet
Size	$250
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse Asc-type amino acid transporter 1 (Slc7a10), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage