Recombinant Mouse Dapper homolog 1 (Dact1)

Code CSB-YP844457MO
MSDS
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Source Yeast
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Code CSB-EP844457MO
MSDS
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Source E.coli
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Code CSB-EP844457MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844457MO
MSDS
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Source Baculovirus
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Code CSB-MP844457MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dact1
Uniprot No.
Alternative Names
Dact1; Thyex3; Dapper homolog 1; Dapper antagonist of catenin 1; Frodo homolog; MDpr1; Thymus-expressed novel gene 3 protein
Species
Mus musculus (Mouse)
Expression Region
1-778
Target Protein Sequence
MKPDAAREPE PLSPGRGAEA EGRWRERGEA DTERQRTRER QEATLAGLAE LGYLRQRQEL LVRGALRCSG TVGTVAPRSG ELRGDAAQRS RLEEKFLEEN ILLLRRQLNC LRRRDAGLLN QLQELDKQIS DLRLDVEKTS EEHLETDSRP SSGFYELSDG ASGSLSNSSN SVFSECLSSC HSSTCFCSPL EAALTISDGC PKSADVNPKY QCDLVSKNGN DVYRYPSPLH AVAVQSPMFL LCLTGNTLRE EEGLGSHASD ICIGSELNAT KTDNSLPSPS SLWSASHPAS SKKMDGYILS LVQKKTHPVR TNKPRTSVNA DPTKGLLRNG SVCVRAPSGV PPGSSVNFKN TKQMCLPAGG ITSLENGPFS PPKQRSKDSK TDQLESKRLA LPESCSAGAA MEPQSKHVPK AAKAASQELT RCQAGLGESM KESNQASAVS PKTSPGRGPV APAESKALQL PKKMSQKNSL QAVPALDRPA LDFKSEGSSQ SLEEGHLVKA QFIPGQQAAA RPHRAHRNPG VARSATLKAR GQAAMEHGLP TVREKPRAAG KKCRFPDDSD TNKKFRKTSA KGRRSGGLQD AGLPGRALGT GGHRAGSRAH AHGREPVVAK PKHKRTDYRR WKSSAEVSYE EALRRARRAR REHGAAYRVA VALPYASPYA YVPSDSEYSA ECESLFHSTV VDTSEDEQSN YTTNCFGDSE SSVSEGDFVG ESTTTSDSEE SGGLIWSQFV QTLPIQTVTA PDLHTRPTKT FVKIKASHNL KKKILRFRSG SLKLMTTV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway. The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.
Gene References into Functions
  1. miR-124 promoted proliferation and induced NSC differentiation to neurons by activation of Wnt/beta-catenin pathway via targeting DACT1, providing a potential target and aiding the development of cell-based therapies for neurological disorders. PMID: 29786763
  2. Dapper1 attenuates hepatic gluconeogenesis and lipogenesis in Ttype 2 diabetes. PMID: 28237722
  3. Dpr1 promotes the ubiquitination of Dvl2 by pVHL and mediates the protein aggregate-elicited autophagy initiation PMID: 25825496
  4. Expression analysis of Dact1-LacZ show high expression levels in multiple mesoderm- and neuroectoderm-derived tissues during embryonic development, but restricted to a small number of postnatal tissues. PMID: 24681206
  5. These results support a novel cell-autonomous postsynaptic role for Dact1, in cooperation with Dishevelled-1 and possibly Disrupted in Schizophrenia-1, in the formation of synapses on cortical interneuron dendrites. PMID: 23826333
  6. Depletion of Dapper-1 and dishevelled-2 in cardiomyocytes demonstrated that Dapper-1 functions upstream of dishevelled-2 and that activity of both Dapper-1 and dishevelled-2 is essential for activating canonical Wnt signaling. PMID: 23509077
  7. conserved role for Dact1 protein in kinase-regulated biochemistry involving Vangl and Dvl. PMID: 21718540
  8. Dact1 was upregulated in the dental follicle mesenchyme at the cap stage and subsequently also in the dental papilla at the bell stage, where the expression persisted to the postnatal stages. PMID: 20170752
  9. Loss of Dact1 disrupts planar cell polarity signaling by altering dishevelled activity and leads to posterior malformation in mice. PMID: 20145239
  10. This study shown that Dact1 plays an important role during dendrite and spine formation in neurons of the mammalian forebrain by promoting activation of Rac PMID: 20335472
  11. Frodo is expressed in primitive streak mesoderm, neuroectoderm, neural crest, presomitic mesoderm, and somites. In many cases, Frodo expression is confined to tissues undergoing extensive morphogenesis PMID: 16278878
  12. Dact1 is detected in the presomitic mesoderm and somites during segmentation, in the limb bud mesenchyme and other mesoderm-derived tissues, and in the central nervous system PMID: 16881060
  13. The oscillation of Dact1 occurs in phase with the Wnt signaling component Axin2, and out of phase with the Notch signaling component Lfng. PMID: 17013874
  14. Dact1 regulates adipogenesis through coordinated effects on gene expression that selectively alter intracellular and paracrine/autocrine components of the Wnt/beta-catenin signaling pathway. PMID: 19073771
  15. Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak. PMID: 19701191

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Subcellular Location
Cytoplasm. Nucleus. Cell junction, synapse.
Protein Families
Dapper family
Tissue Specificity
Expressed in multiple tissues including brain, heart, kidney, liver and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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